 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 7357-7366, Copyright © 1995 by Society for Neuroscience
A timetable of events during programmed cell death induced by trophic factor withdrawal from neuronal PC12 cells
PW Mesner, CL Epting, JL Hegarty and SH Green
Department of Biological Sciences, University of Iowa, Iowa City 52242- 1324, USA.
We describe a timetable of events during programmed cell death (PCD) in
neuronal PC12 cells, specifically, Ras signaling, immediate-early gene
(IEG) expression, DNA fragmentation and commitment to PCD. Commitment
occurs over a period from 10-20 hr after NGF withdrawal. Ras signaling
declines rapidly after NGF removal, reaching minimal levels within 2-4 hr,
well before the onset of commitment. DNA fragmentation, detected by TUNEL
reaction, begins about 24 hr after NGF withdrawal, well after all cells are
committed, but coincident with the onset of cell dissolution previously
determined by trypan blue exclusion (Mesner et al., 1992). Among the IEGs
studied here, c-jun and TIS21 are expressed within 6 hr after NGF
withdrawal. Expression of c-fos, egr-1, and TIS11 does not begin until 20
hr after NGF withdrawal. IEG expression generally ends by 24 hr after NGF
withdrawal. The IEGs TIS7 and nur77 are not expressed during PCD, yielding
a pattern distinct from that following other stimuli. An identical pattern
of IEG expression occurs in non- neuronal PC12 cells deprived of serum,
although expression begins at 10- 14 hr after serum withdrawal. A similar
IEG expression pattern was observed in Rat-1 fibroblasts, with various
genes expressed 6-18 hr after serum withdrawal. In none of these cell types
did expression of the stress-related gene Hsp70 change following trophic
factor withdrawal. The distinctive pattern of IEG expression described here
should facilitate identification of intracellular regulatory signals active
during PCD.
This article has been cited by other articles:
|
|
|
|
 
C. A. Marques, U. Keil, A. Bonert, B. Steiner, C. Haass, W. E. Muller, and A. Eckert
Neurotoxic Mechanisms Caused by the Alzheimer's Disease-linked Swedish Amyloid Precursor Protein Mutation: OXIDATIVE STRESS, CASPASES, AND THE JNK PATHWAY
J. Biol. Chem.,
July 18, 2003;
278(30):
28294 - 28302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G Thuret, C Chiquet, L Campos, and P Gain
Dynamics of corneal endothelial cell death in organ culture
Br. J. Ophthalmol.,
March 1, 2003;
87
(3):
376 - 377.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Worthington, J. W. Pelo, M. A. Sachedina, J. L. Applegate, K. O. Arseneau, and T. T. Pizarro
RNA Binding Properties of the AU-rich Element-binding Recombinant Nup475/TIS11/Tristetraprolin Protein
J. Biol. Chem.,
December 6, 2002;
277(50):
48558 - 48564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wei, D. D. Norton, X. Wang, and J. W. Kusiak
A{beta} 17-42 in Alzheimer's disease activates JNK and caspase-8 leading to neuronal apoptosis
Brain,
September 1, 2002;
125(9):
2036 - 2043.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Johnson, J. R. Stehn, M. B. Yaffe, and T. K. Blackwell
Cytoplasmic Localization of Tristetraprolin Involves 14-3-3-dependent and -independent Mechanisms
J. Biol. Chem.,
May 10, 2002;
277(20):
18029 - 18036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. G. Tatton, R. M. E. Chalmers-Redman, W. J. H. Ju, M. Mammen, G. W. Carlile, A. W. Pong, and N. A. Tatton
Propargylamines Induce Antiapoptotic New Protein Synthesis in Serum- and Nerve Growth Factor (NGF)-Withdrawn, NGF-Differentiated PC-12 Cells
J. Pharmacol. Exp. Ther.,
May 1, 2002;
301(2):
753 - 764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Culman, J. Baulmann, A. Blume, and T. Unger
Review: The renin-angiotensin system in the brain: an update
Journal of Renin-Angiotensin-Aldosterone System,
June 1, 2001;
2(2):
96 - 102.
[PDF]
|
|
|
|
|
|
|
M. R. Hansen, X.-M. Zha, J. Bok, and S. H. Green
Multiple Distinct Signal Pathways, Including an Autocrine Neurotrophic Mechanism, Contribute to the Survival-Promoting Effect of Depolarization on Spiral Ganglion Neurons In Vitro
J. Neurosci.,
April 1, 2001;
21(7):
2256 - 2267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Namgung and Z. Xia
Arsenite-Induced Apoptosis in Cortical Neurons Is Mediated by c-Jun N-Terminal Protein Kinase 3 and p38 Mitogen-Activated Protein Kinase
J. Neurosci.,
September 1, 2000;
20(17):
6442 - 6451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Irie, K. Yamagata, Y. Gan, K. Miyamoto, E. Do, C.-H. Kuo, E. Taira, and N. Miki
Molecular Cloning and Characterization of Amida, a Novel Protein Which Interacts with a Neuron-specific Immediate Early Gene Product Arc, Contains Novel Nuclear Localization Signals, and Causes Cell Death in Cultured Cells
J. Biol. Chem.,
January 28, 2000;
275(4):
2647 - 2653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Prevot, T. Voeltzel, A.-M. Birot, A.-P. Morel, M.-C. Rostan, J.-P. Magaud, and L. Corbo
The Leukemia-associated Protein Btg1 and the p53-regulated Protein Btg2 Interact with the Homeoprotein Hoxb9 and Enhance Its Transcriptional Activation
J. Biol. Chem.,
January 7, 2000;
275(1):
147 - 153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Nicholls and S. L. Budd
Mitochondria and Neuronal Survival
Physiol Rev,
January 1, 2000;
80(1):
315 - 360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Pelletier, J. S. Wadia, L. R. Mills, and P. L. Carlen
Seizure-Induced Cell Death Produced by Repeated Tetanic Stimulation In Vitro: Possible Role of Endoplasmic Reticulum Calcium Stores
J Neurophysiol,
June 1, 1999;
81(6):
3054 - 3064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Le-Niculescu, E. Bonfoco, Y. Kasuya, F.-X. Claret, D. R. Green, and M. Karin
Withdrawal of Survival Factors Results in Activation of the JNK Pathway in Neuronal Cells Leading to Fas Ligand Induction and Cell Death
Mol. Cell. Biol.,
January 1, 1999;
19(1):
751 - 763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Rouault, D. Prevot, C. Berthet, A.-M. Birot, M. Billaud, J.-P. Magaud, and L. Corbo
Interaction of BTG1 and p53-regulated BTG2 Gene Products with mCaf1, the Murine Homolog of a Component of the Yeast CCR4 Transcriptional Regulatory Complex
J. Biol. Chem.,
August 28, 1998;
273(35):
22563 - 22569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Y. Barnes, L. Li, K. Yoshikawa, L. M. Schwartz, R. W. Oppenheim, and C. E. Milligan
Increased Production of Amyloid Precursor Protein Provides a Substrate for Caspase-3 in Dying Motoneurons
J. Neurosci.,
August 1, 1998;
18(15):
5869 - 5880.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Wadia, R. M. E. Chalmers-Redman, W. J. H. Ju, G. W. Carlile, J. L. Phillips, A. D. Fraser, and W. G. Tatton
Mitochondrial Membrane Potential and Nuclear Changes in Apoptosis Caused by Serum and Nerve Growth Factor Withdrawal: Time Course and Modification by (-)-Deprenyl
J. Neurosci.,
February 1, 1998;
18(3):
932 - 947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Watson, A. Eilers, D. Lallemand, J. Kyriakis, L. L. Rubin, and J. Ham
Phosphorylation of c-Jun Is Necessary for Apoptosis Induced by Survival Signal Withdrawal in Cerebellar Granule Neurons
J. Neurosci.,
January 15, 1998;
18(2):
751 - 762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Deshmukh and E. M. Johnson Jr.
Programmed Cell Death in Neurons: Focus on the Pathway of Nerve Growth Factor Deprivation-Induced Death of Sympathetic Neurons
Mol. Pharmacol.,
June 1, 1997;
51(6):
897 - 906.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Z. Pang and J. W. Geddes
Mechanisms of Cell Death Induced by the Mitochondrial Toxin 3-Nitropropionic Acid: Acute Excitotoxic Necrosis and Delayed Apoptosis
J. Neurosci.,
May 1, 1997;
17(9):
3064 - 3073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Furukawa, S. Estus, W. Fu, R. J. Mark, and M. P. Mattson
Neuroprotective Action of Cycloheximide Involves Induction of Bcl-2 and Antioxidant Pathways
J. Cell Biol.,
March 10, 1997;
136(5):
1137 - 1149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Wolozin, K. Iwasaki, P. Vito, J. K. Ganjei, E. Lacanà, T. Sunderland, B. Zhao, J. W. Kusiak, W. Wasco, and L. D'Adamio
Participation of Presenilin 2 in Apoptosis: Enhanced Basal Activity Conferred by an Alzheimer Mutation
Science,
December 6, 1996;
274(5293):
1710 - 1713.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Prevot, A.-P. Morel, T. Voeltzel, M.-C. Rostan, R. Rimokh, J.-P. Magaud, and L. Corbo
Relationships of the Antiproliferative Proteins BTG1 and BTG2 with CAF1, the Human Homolog of a Component of the Yeast CCR4 Transcriptional Complex. INVOLVEMENT IN ESTROGEN RECEPTOR alpha SIGNALING PATHWAY
J. Biol. Chem.,
March 23, 2001;
276(13):
9640 - 9648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
