 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 7861-7871, Copyright © 1995 by Society for Neuroscience
ATP-induced cytoplasmic calcium mobilization in Bergmann glial cells
S Kirischuk, T Moller, N Voitenko, H Kettenmann and A Verkhratsky
Bogomoletz Institute of Physiology, Kiev-24, Ukraine.
ATP receptor mediated Ca2+ signaling was recorded from Bergmann glial cells
in cerebellar slices obtained from mice of different ages (postnatal days 6
to 45). To measure the cytoplasmic concentration of Ca2+ ([Ca2+]in), either
individual cells were loaded with the Ca(2+)- sensitive probes using the
whole cell patch clamp technique or slices were incubated with the dye and
the microfluorimetric system was focused on individual cells. Signals were
recorded either with single- detector microfluorimetry of the dye fura-2 or
by confocal laser scanning microfluorimetry (fluo-3-based recordings).
Extracellular application of 100 microns ATP caused a transient elevation
of [Ca2+]in, which amplitude was significantly higher in Bergmann glial
cell processes as compared with their soma. The rank order of potency for
the purinoreceptor agonists was: ADP > or = ATP > UTP >> AMP =
adenosine = alpha, beta-methylene-ATP. ATP-triggered Ca2+ transients were
reversibly inhibited by the P2 purinoreceptor agonist suramin (100 microM).
The involvement of P2 metabotropic receptors is inferred by the observation
that ATP mediated cytoplasmic Ca2+ transients were not associated with a
measurable change in membrane conductance. The [Ca2+]in increase was due to
release from inositol-1,4,5-trisphosphate (InsP3)-sensitive intracellular
stores since responses were still observed in Ca(2+)-free extracellular
solutions and were irreversibly blocked by the inhibitor of the
sarco(endo)plasmic reticulum Ca2+ ATPase, thapsigargin, and by the
competitive inhibitor of the InsP3- gated intracellular Ca2+ channels
heparin. Intracellular dialysis altered the refilling process of the
InsP3-sensitive stores, suggesting that cytoplasmic factors control ATP
mediated Ca2+ signalling.
This article has been cited by other articles:
|
|
|
|
 
M. Doengi, J. W. Deitmer, and C. Lohr
New evidence for purinergic signaling in the olfactory bulb: A2A and P2Y1 receptors mediate intracellular calcium release in astrocytes
FASEB J,
July 1, 2008;
22(7):
2368 - 2378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Lalo, Y. Pankratov, S. P. Wichert, M. J. Rossner, R. A. North, F. Kirchhoff, and A. Verkhratsky
P2X1 and P2X5 Subunits Form the Functional P2X Receptor in Mouse Cortical Astrocytes
J. Neurosci.,
May 21, 2008;
28(21):
5473 - 5480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Piet and C. E. Jahr
Glutamatergic and Purinergic Receptor-Mediated Calcium Transients in Bergmann Glial Cells
J. Neurosci.,
April 11, 2007;
27(15):
4027 - 4035.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Singaravelu, C. Lohr, and J. W. Deitmer
Regulation of store-operated calcium entry by calcium-independent phospholipase A2 in rat cerebellar astrocytes.
J. Neurosci.,
September 13, 2006;
26(37):
9579 - 9592.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Beierlein and W. G. Regehr
Brief bursts of parallel fiber activity trigger calcium signals in bergmann glia.
J. Neurosci.,
June 28, 2006;
26(26):
6958 - 6967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Saitow, T. Murakoshi, H. Suzuki, and S. Konishi
Metabotropic P2Y Purinoceptor-Mediated Presynaptic and Postsynaptic Enhancement of Cerebellar GABAergic Transmission
J. Neurosci.,
February 23, 2005;
25(8):
2108 - 2116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Cinelli, D. Wang, P. Chen, W. Liu, and M. Halpern
Calcium Transients in the Garter Snake Vomeronasal Organ
J Neurophysiol,
March 1, 2002;
87(3):
1449 - 1472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kulik, A. Haentzsch, M. Luckermann, W. Reichelt, and K. Ballanyi
Neuron-Glia Signaling via alpha 1 Adrenoceptor-Mediated Ca2+ Release in Bergmann Glial Cells In Situ
J. Neurosci.,
October 1, 1999;
19(19):
8401 - 8408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ralevic and G. Burnstock
Receptors for Purines and Pyrimidines
Pharmacol. Rev.,
September 1, 1998;
50(3):
413 - 492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Mateo, M. Garcia-Lecea, M. T. Miras-Portugal, and E. Castro
Ca2+ Signals Mediated by P2X-Type Purinoceptors in Cultured Cerebellar Purkinje Cells
J. Neurosci.,
March 1, 1998;
18(5):
1704 - 1712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. VERKHRATSKY, R. K. ORKAND, and H. KETTENMANN
Glial Calcium: Homeostasis and Signaling Function
Physiol Rev,
January 1, 1998;
78(1):
99 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
