QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kiryu, S. Articles by Kiyama, H. Search for Related Content PubMed PubMed Citation Articles by Kiryu, S. Articles by Kiyama, H.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 15, 7872-7878, Copyright © 1995 by Society for Neuroscience

ARTICLE

Nerve injury enhances rat neuronal glutamate transporter expression: identification by differential display PCR

S Kiryu, GL Yao, N Morita, H Kato and H Kiyama
Department of Neuroanatomy, Osaka University Medical School, Japan.

An increase in neuronal glutamate transporter expression after nerve injury was demonstrated by means of differential display PCR (DD-PCR) coupled with in situ hybridization. DD-PCR was carried out to compare differences in expression of mRNAs between axotomized and normal hypoglossal motoneurons in the rat. The expression of several gene fragments were found to be increased following nerve injury; the full length cDNA corresponding to one fragment was cloned by subsequent rat cDNA library screening. The close homology of glutamate transporters with our rat cDNA led us to conclude that this clone corresponds to the rat neuronal glutamate transporter (rat EAAC1). We speculate that the upregulation of this glutamate uptake system may increase the resistance of these cells against neurotoxic glutamate accumulation during the process of nerve regeneration.

This article has been cited by other articles:

				S. Kiryu-Seo, R. Kato, T. Ogawa, S. Nakagomi, K. Nagata, and H. Kiyama Neuronal Injury-inducible Gene Is Synergistically Regulated by ATF3, c-Jun, and STAT3 through the Interaction with Sp1 in Damaged Neurons J. Biol. Chem., March 14, 2008; 283(11): 6988 - 6996. [Abstract] [Full Text] [PDF]

				S. Kiryu-Seo, T. Hirayama, R. Kato, and H. Kiyama Noxa Is a Critical Mediator of p53-Dependent Motor Neuron Death after Nerve Injury in Adult Mouse J. Neurosci., February 9, 2005; 25(6): 1442 - 1447. [Abstract] [Full Text] [PDF]

				K. Tanabe, I. Bonilla, J. A. Winkles, and S. M. Strittmatter Fibroblast Growth Factor-Inducible-14 Is Induced in Axotomized Neurons and Promotes Neurite Outgrowth J. Neurosci., October 22, 2003; 23(29): 9675 - 9686. [Abstract] [Full Text] [PDF]

				S. Aoki, Q. Su, H. Li, K. Nishikawa, K. Ayukawa, Y. Hara, K. Namikawa, S. Kiryu-Seo, H. Kiyama, and K. Wada Identification of an Axotomy-Induced Glycosylated Protein, AIGP1, Possibly Involved in Cell Death Triggered by Endoplasmic Reticulum-Golgi Stress J. Neurosci., December 15, 2002; 22(24): 10751 - 10760. [Abstract] [Full Text] [PDF]

				I. E. Bonilla, K. Tanabe, and S. M. Strittmatter Small Proline-Rich Repeat Protein 1A Is Expressed by Axotomized Neurons and Promotes Axonal Outgrowth J. Neurosci., February 15, 2002; 22(4): 1303 - 1315. [Abstract] [Full Text] [PDF]

				K. Tanabe, T. Tachibana, T. Yamashita, Y. H. Che, Y. Yoneda, T. Ochi, M. Tohyama, H. Yoshikawa, and H. Kiyama The Small GTP-Binding Protein TC10 Promotes Nerve Elongation in Neuronal Cells, and Its Expression Is induced during Nerve Regeneration in Rats J. Neurosci., June 1, 2000; 20(11): 4138 - 4144. [Abstract] [Full Text] [PDF]

				K. Namikawa, M. Honma, K. Abe, M. Takeda, K. Mansur, T. Obata, A. Miwa, H. Okado, and H. Kiyama Akt/Protein Kinase B Prevents Injury-Induced Motoneuron Death and Accelerates Axonal Regeneration J. Neurosci., April 15, 2000; 20(8): 2875 - 2886. [Abstract] [Full Text] [PDF]

				G. W. Mathern, D. Mendoza, A. Lozada, J. K. Pretorius, Y. Dehnes, N. C. Danbolt, N. Nelson, J. P. Leite, L. Chimelli, D. E. Born, et al. Hippocampal GABA and glutamate transporter immunoreactivity in patients with temporal lobe epilepsy Neurology, February 1, 1999; 52(3): 453 - 453. [Abstract] [Full Text] [PDF]

				R. Tal-Singer, W. Podrzucki, T. M. Lasner, A. Skokotas, J. J. Leary, N. W. Fraser, and S. L. Berger Use of Differential Display Reverse Transcription-PCR To Reveal Cellular Changes during Stimuli That Result in Herpes Simplex Virus Type 1 Reactivation from Latency: Upregulation of Immediate-Early Cellular Response Genes TIS7, Interferon, and Interferon Regulatory Factor-1 J. Virol., February 1, 1998; 72(2): 1252 - 1261. [Abstract] [Full Text] [PDF]

				T. Shibata, K. Yamada, M. Watanabe, K. Ikenaka, K. Wada, K. Tanaka, and Y. Inoue Glutamate Transporter GLAST Is Expressed in the Radial Glia-Astrocyte Lineage of Developing Mouse Spinal Cord J. Neurosci., December 1, 1997; 17(23): 9212 - 9219. [Abstract] [Full Text] [PDF]

				N. Morita, S. Kiryu, and a. H. Kiyama p53-Independent Cyclin G Expression in a Group of Mature Neurons and Its Enhanced Expression during Nerve Regeneration J. Neurosci., October 1, 1996; 16(19): 5961 - 5966. [Abstract] [Full Text] [PDF]

				S. Kiryu-Seo, M. Sasaki, H. Yokohama, S. Nakagomi, T. Hirayama, S. Aoki, K. Wada, and H. Kiyama Damage-induced neuronal endopeptidase (DINE) is a unique metallopeptidase expressed in response to neuronal damage and activates superoxide scavengers PNAS, April 11, 2000; 97(8): 4345 - 4350. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help