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Journal of Neuroscience, Vol 15, 8177-8190, Copyright © 1995 by Society for Neuroscience
Role of neural activity during synaptogenesis in Drosophila
J Jarecki and H Keshishian
Department of Genetics, Yale University, New Haven, Connecticut 06511, USA.
This article reveals a novel aspect in the regulation of synaptic
connectivity in Drosophila. Reducing neural activity genetically or
pharmacologically disrupts the normally precise embryonic and larval
neuromuscular connections. In third instar larvae with mutations that
affect sodium channel function or expression such as no action potential,
temperature-induced paralysis E, or seizure1, foreign neuromuscular
synapses, arising from inappropriate nerve sources, are observed on muscle
fibers throughout the abdominal body wall. Their frequencies increase as
neural activity is further reduced in double mutant combinations. These
foreign connections are first observed during late embryogenesis as
filopodial-like contacts, but critical period analysis suggests that neural
activity must be reduced during both late embryogenesis and the first
larval instar to promote the differentiation of these embryonic contacts
into foreign motor synapses. In addition, the loss of electrical activity
in the motoneuron, as opposed to the loss of postsynaptic potentials in the
muscle fibers, appears to be responsible for these changes in connectivity.
Our experiments suggest that neural activity may function during
development by preventing inappropriate connections and thereby maintaining
the precise connectivity achieved during nerve outgrowth and target
selection.
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