 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 1110-1120, Copyright © 1995 by Society for Neuroscience
Dynamics of granule cell migration: a confocal microscopic study in acute cerebellar slice preparations
H Komuro and P Rakic
Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06510.
Real-time examination of Dil-labeled, immature granule cells in cerebellar
slice preparations reveals several temporal and cytological aspects of
neuronal migration that have not been observed in previous in vivo or in
vitro systems. Using confocal microscopy we have obtained evidence that
rates of cell movement depend critically on the age of the cerebellum.
Although there were considerable variations in the speed of individual
cells, the average rate of cell migration increased systematically from 9.6
+/- 3.0 microns/hr in cerebella from 7-d-old mice to 18.0 +/- 2.9
microns/hr in cerebella from 13-d-old mice. Consequently, granule cells
traversed the developing molecular layer within a relatively constant time
period despite the doubling in width of the molecular layer during the
second week of postnatal life. Granule cell movement was characterized by
alternations of short stationary phases with movement in a forward or
backward direction. The net displacement of a cell depended on the duration
and frequency of these phases as well as on the speed of movement. Changes
in the relative position of Dil crystals attached to the surface of granule
cells suggested the existence of a complex topographical flow of plasma
membrane during migration. Although a large portion of the plasma membrane
seemed to move in register with the nucleus and surrounding cytoplasm, new
membrane appeared to be incorporated primarily at the leading process.
However, the pattern of membrane flow at the interface between migrating
neurons and Bergmann glial fibers could not be determined, since these
sites could not be labeled by Dil crystals. The present results are in
harmony with the concept that multiple cellular/molecular mechanisms may be
engaged in granule cell migration.
This article has been cited by other articles:
|
|
|
|
 
H. Umeshima, T. Hirano, and M. Kengaku
Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons
PNAS,
October 9, 2007;
104(41):
16182 - 16187.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Borrell, B. K. Kaspar, F. H. Gage, and E. M. Callaway
In vivo Evidence for Radial Migration of Neurons by Long-Distance Somal Translocation in the Developing Ferret Visual Cortex
Cereb Cortex,
November 1, 2006;
16(11):
1571 - 1583.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. M. Morozov, A. E. Ayoub, and P. Rakic
Translocation of Synaptically Connected Interneurons across the Dentate Gyrus of the Early Postnatal Rat Hippocampus.
J. Neurosci.,
May 10, 2006;
26(19):
5017 - 5027.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kumada, M. K. Lakshmana, and H. Komuro
Reversal of Neuronal Migration in a Mouse Model of Fetal Alcohol Syndrome by Controlling Second-Messenger Signalings
J. Neurosci.,
January 18, 2006;
26(3):
742 - 756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kumada and H. Komuro
Completion of neuronal migration regulated by loss of Ca2+ transients
PNAS,
June 1, 2004;
101(22):
8479 - 8484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Yacubova and H. Komuro
Intrinsic Program for Migration of Cerebellar Granule Cells In Vitro
J. Neurosci.,
July 15, 2002;
22(14):
5966 - 5981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hamasaki, S. Goto, S. Nishikawa, and Y. Ushio
Early-generated Preplate Neurons in the Developing Telencephalon: Inward Migration into the Developing Striatum
Cereb Cortex,
May 1, 2001;
11(5):
474 - 484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Komuro, E. Yacubova, E. Yacubova, and P. Rakic
Mode and Tempo of Tangential Cell Migration in the Cerebellar External Granular Layer
J. Neurosci.,
January 15, 2001;
21(2):
527 - 540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. W. Seeds, M. E. Basham, and S. P. Haffke
Neuronal migration is retarded in mice lacking the tissue plasminogen activator gene
PNAS,
November 23, 1999;
96(24):
14118 - 14123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Mulrenin, J. W. Witkin, and A.-J. Silverman
Embryonic Development of the Gonadotropin-Releasing Hormone (GnRH) System in the Chick: A Spatio-Temporal Analysis of GnRH Neuronal Generation, Site of Origin, and Migration
Endocrinology,
January 1, 1999;
140(1):
422 - 433.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Komuro and P. Rakic
Distinct Modes of Neuronal Migration in Different Domains of Developing Cerebellar Cortex
J. Neurosci.,
February 15, 1998;
18(4):
1478 - 1490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ware, A Wells, and D. Lauffenburger
Epidermal growth factor alters fibroblast migration speed and directional persistence reciprocally and in a matrix-dependent manner
J. Cell Sci.,
January 8, 1998;
111(16):
2423 - 2432.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. C. Carlson, M. L. Slawecki, E. Lancaster, and A. Keller
Distribution and Activation of Intracellular Ca2+ Stores in Cultured Olfactory Bulb Neurons
J Neurophysiol,
October 1, 1997;
78(4):
2176 - 2185.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Phelps, R. Barber, and J. Vaughn
Nonradial migration of interneurons can be experimentally altered in spinal cord slice cultures
Development,
January 7, 1996;
122(7):
2013 - 2022.
[Abstract]
[PDF]
|
|
|
|
