 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 1132-1143, Copyright © 1995 by Society for Neuroscience
Cloning and characterization of a calcium channel alpha 1 subunit from Drosophila melanogaster with similarity to the rat brain type D isoform
W Zheng, G Feng, D Ren, DF Eberl, F Hannan, M Dubald and LM Hall
State University of New York at Buffalo, Department of Biochemical Pharmacology 14260.
We report the complete sequence of a calcium channel alpha 1 subunit cDNA
cloned from a Drosophila head cDNA library. This cDNA encodes a deduced
protein containing 2516 amino acids with a predicted molecular weight of
276,493. The deduced protein shares many features with vertebrate homologs,
including four repeat structures, each containing six transmembrane
domains, a conserved ion selectivity filter region between transmembrane
domains 5 and 6, and an EF hand in the carboxy tail. The Drosophila subunit
has unusually long initial amino and terminal carboxy tails. The region
corresponding to the last transmembrane domain (IVS6) and the adjacent
cytoplasmic domain has been postulated to form a phenylalkylamine-binding
site in vertebrate calcium channels. This region is conserved in the
Drosophila sequence, while domains thought to be involved in
dihydropyridine binding show numerous changes. The Drosophila subunit
exhibits 78.3% sequence similarity to the rat brain type D calcium channel
alpha 1 subunit, and so has been designated as a Drosophila melanogaster
calcium channel alpha 1 type D subunit (Dmca1D). In situ hybridization
shows that Dmca1D is highly expressed in the embryonic nervous system.
Northern analysis shows that Dmca1D cDNA hybridizes to three size classes
of mRNA (9.5, 10.2, and 12.5 kb) in heads, but only two classes (9.5 and
12.5 kb) in bodies and legs. PCR analysis suggests that the Dmca1D message
undergoes alternative splicing with more heterogeneity appearing in head
and embryonic extracts than in bodies and legs.
This article has been cited by other articles:
|
|
|
|
 
S. A. Davies and S. Terhzaz
Organellar calcium signalling mechanisms in Drosophila epithelial function
J. Exp. Biol.,
February 1, 2009;
212(3):
387 - 400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. V. Ly, C.-K. Yao, P. Verstreken, T. Ohyama, and H. J. Bellen
straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel {alpha}1 subunit
J. Cell Biol.,
April 3, 2008;
181(1):
157 - 170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I-F. Peng and C.-F. Wu
Drosophila cacophony Channels: A Major Mediator of Neuronal Ca2+ Currents and a Trigger for K+ Channel Homeostatic Regulation
J. Neurosci.,
January 31, 2007;
27(5):
1072 - 1081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Wang, K. M. C. Sullivan, and K. Beckingham
Drosophila Calmodulin Mutants With Specific Defects in the Musculature or in the Nervous System
Genetics,
November 1, 2003;
165(3):
1255 - 1268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Chan, A. Villella, P. Funes, and J. C. Hall
Courtship and Other Behaviors Affected by a Heat-Sensitive, Molecularly Novel Mutation in the cacophony Calcium-Channel Gene of Drosophila
Genetics,
September 1, 2002;
162(1):
135 - 153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Kawasaki, S. C. Collins, and R. W. Ordway
Synaptic Calcium-Channel Function in Drosophila: Analysis and Transformation Rescue of Temperature-Sensitive Paralytic and Lethal Mutations of Cacophony
J. Neurosci.,
July 15, 2002;
22(14):
5856 - 5864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. MacPherson, V. P. Pollock, K. E. Broderick, L. Kean, F. C. O'Connell, J. A. T. Dow, and S. A. Davies
Model Organisms: New Insights Into Ion Channel and Transporter Function.: L-type calcium channels regulate epithelial fluid transport in Drosophila melanogaster
Am J Physiol Cell Physiol,
February 1, 2001;
280(2):
C394 - C407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jeziorski, R. Greenberg, and P. Anderson
The molecular biology of invertebrate voltage-gated Ca(2+) channels
J. Exp. Biol.,
January 3, 2000;
203(5):
841 - 856.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Ashburner, S. Misra, J. Roote, S. E. Lewis, R. Blazej, T. Davis, C. Doyle, R. Galle, R. George, N. Harris, et al.
An Exploration of the Sequence of a 2.9-Mb Region of the Genome of Drosophila melanogaster: The Adh Region
Genetics,
September 1, 1999;
153(1):
179 - 219.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. C. Jeziorski, R. M. Greenberg, K. S. Clark, and P. A. V. Anderson
Cloning and Functional Expression of a Voltage-gated Calcium Channel alpha 1 Subunit from Jellyfish
J. Biol. Chem.,
August 28, 1998;
273(35):
22792 - 22799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Smith, A. A. Peixoto, E. M. Kramer, A. Villella, and J. C. Hall
Courtship and Visual Defects of cacophony Mutants Reveal Functional Complexity of a Calcium-Channel {alpha}1 Subunit in Drosophila
Genetics,
July 1, 1998;
149(3):
1407 - 1426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Baines and M. Bate
Electrophysiological Development of Central Neurons in the Drosophila Embryo
J. Neurosci.,
June 15, 1998;
18(12):
4673 - 4683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Hurley and K. Graubard
Pharmacologically and Functionally Distinct Calcium Currents of Stomatogastric Neurons
J Neurophysiol,
April 1, 1998;
79(4):
2070 - 2081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ren, H. Xu, D. F. Eberl, M. Chopra, and L. M. Hall
A Mutation Affecting Dihydropyridine-Sensitive Current Levels and Activation Kinetics in Drosophila Muscle and Mammalian Heart Calcium Channels
J. Neurosci.,
April 1, 1998;
18(7):
2335 - 2341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. F. Eberl, D. Ren, G. Feng, L. J. Lorenz, D. Van Vactor, and L. M. Hall
Genetic and Developmental Characterization of Dmca1D, a Calcium Channel {alpha}1 Subunit Gene in Drosophila melanogaster
Genetics,
March 1, 1998;
148(3):
1159 - 1169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Peixoto and J. C. Hall
Analysis of Temperature-Sensitive Mutants Reveals New Genes Involved in the Courtship Song of Drosophila
Genetics,
February 1, 1998;
148(2):
827 - 838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ren and L. M. Hall
Functional Expression and Characterization of Skeletal Muscle Dihydropyridine Receptors in Xenopus Oocytes
J. Biol. Chem.,
September 5, 1997;
272(36):
22393 - 22396.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. H. White and L. K. Kaczmarek
Identification of a Vesicular Pool of Calcium Channels in the Bag Cell Neurons of Aplysia californica
J. Neurosci.,
March 1, 1997;
17(5):
1582 - 1595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, E. R. Reynolds, P. Deak, and L. M. Hall
The seizure Locus Encodes the Drosophila Homolog of the HERG Potassium Channel
J. Neurosci.,
February 1, 1997;
17(3):
882 - 890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G Gellon, K. Harding, N McGinnis, M. Martin, and W McGinnis
A genetic screen for modifiers of Deformed homeotic function identifies novel genes required for head development
Development,
January 9, 1997;
124(17):
3321 - 3331.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. A. Smith, X. Wang, A. A. Peixoto, E. K. Neumann, L. M. Hall, and J. C. Hall
A Drosophila Calcium Channel alpha 1 Subunit Gene Maps to a Genetic Locus Associated with Behavioral and Visual Defects
J. Neurosci.,
December 15, 1996;
16(24):
7868 - 7879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Feng, F. Hannan, V. Reale, Y. Y. Hon, C. T. Kousky, P. D. Evans, and L. M. Hall
Cloning and Functional Characterization of a Novel Dopamine Receptor from Drosophila melanogaster
J. Neurosci.,
June 15, 1996;
16(12):
3925 - 3933.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
