 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 2524-2532, Copyright © 1995 by Society for Neuroscience
Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents
RH Adams, K Sato, S Shimada, M Tohyama, AW Puschel and H Betz
Abteilung Neurochemie, Max-Planck-Institut fur Hirnforschung, Frankfurt/Main, Germany.
Na+/Cl(-)-dependent glycine transporters are crucial for the termination of
neurotransmission at glycinergic synapses. Two different glycine
transporter genes, GlyT1 and GlyT2, have been described. Several isoforms
differing in their 5' ends originate from the GlyT1 gene. We have
determined the genomic structure of the murine GlyT1 gene to elucidate the
genetic basis underlying the different isoforms. Analysis of cDNA 5'-ends
revealed that the GlyT1a and 1b/1c mRNAs are transcribed from two different
promoters. During murine embryonic development GlyT1 mRNAs were detectable
by RNase protection assays as early as embryonic day E9 and reached maximal
levels between E13 and E15. In situ hybridization revealed GlyT1 expression
in the developing spinal cord mainly in the ventral part of the ventricular
zone at E12. At later stages (E15) transcripts were also found in the
lateral half of the basal and intermediate gray matter. In contrast, the
second glycine transporter gene GlyT2 displayed a completely different
expression pattern. At E11 it is expressed in the mantle zone, and at later
stages throughout the ventral horns. In the adult rat brain and spinal
cord, GlyT1 hybridization signals were found exclusively in glial cells.
Our data indicate that GlyT1 is an early marker of neural development and
encodes glia-specific transporter proteins.
This article has been cited by other articles:
|
|
|
|
 
E. Kartvelishvily, M. Shleper, L. Balan, E. Dumin, and H. Wolosker
Neuron-derived D-Serine Release Provides a Novel Means to Activate N-Methyl-D-aspartate Receptors
J. Biol. Chem.,
May 19, 2006;
281(20):
14151 - 14162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. W. Cui, S. E. Low, H. Hirata, L. Saint-Amant, R. Geisler, R. I. Hume, and J. Y. Kuwada
The Zebrafish shocked Gene Encodes a Glycine Transporter and Is Essential for the Function of Early Neural Circuits in the CNS
J. Neurosci.,
July 13, 2005;
25(28):
6610 - 6620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. R. Benjamin, J. Skelton, D. Hanway, S. Olanrewaju, F. Pruthi, V. I. Ilyin, D. Lavery, S. F. Victory, and K. J. Valenzano
Validation of a Fluorescent Imaging Plate Reader Membrane Potential Assay for High-Throughput Screening of Glycine Transporter Modulators
J Biomol Screen,
June 1, 2005;
10(4):
365 - 373.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H. Huang, L. Barakat, D. Wang, and A. Bordey
Bergmann glial GlyT1 mediates glycine uptake and release in mouse cerebellar slices
J. Physiol.,
November 1, 2004;
560(3):
721 - 736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Lim, P. Hoang, and A. J. Berger
Blockade of Glycine Transporter-1 (GLYT-1) Potentiates NMDA Receptor-Mediated Synaptic Transmission in Hypoglossal Motorneurons
J Neurophysiol,
October 1, 2004;
92(4):
2530 - 2537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bradaia, R. Schlichter, and J. Trouslard
Role of glial and neuronal glycine transporters in the control of glycinergic and glutamatergic synaptic transmission in lamina X of the rat spinal cord
J. Physiol.,
August 15, 2004;
559(1):
169 - 186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. West, M. A. Castellini, M. K. Duncan, and M. Bustin
Chromosomal Proteins HMGN3a and HMGN3b Regulate the Expression of Glycine Transporter 1
Mol. Cell. Biol.,
May 1, 2004;
24(9):
3747 - 3756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yang, W. Ge, Y. Chen, Z. Zhang, W. Shen, C. Wu, M. Poo, and S. Duan
Contribution of astrocytes to hippocampal long-term potentiation through release of D-serine
PNAS,
December 9, 2003;
100(25):
15194 - 15199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Steeves, M.-A. Hammer, G. B. Walker, D. Rae, N. A. Stewart, and J. M. Baltz
The glycine neurotransmitter transporter GLYT1 is an organic osmolyte transporter regulating cell volume in cleavage-stage embryos
PNAS,
November 25, 2003;
100(24):
13982 - 13987.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Chen, M. Muhlhauser, and C. R. Yang
Glycine Tranporter-1 Blockade Potentiates NMDA-Mediated Responses in Rat Prefrontal Cortical Neurons In Vitro and In Vivo
J Neurophysiol,
February 1, 2003;
89(2):
691 - 703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Horiuchi, A. Nicke, J. Gomeza, A. Aschrafi, G. Schmalzing, and H. Betz
Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopus oocytes
PNAS,
February 1, 2001;
(2001)
41329498.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Ponce, B. Biton, J. Benavides, P. Avenet, and C. Aragon
Transmembrane Domain III Plays an Important Role in Ion Binding and Permeation in the Glycine Transporter GLYT2
J. Biol. Chem.,
April 28, 2000;
275(18):
13856 - 13862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. T. Coyle and R. Schwarcz
Mind Glue: Implications of Glial Cell Biology for Psychiatry
Arch Gen Psychiatry,
January 1, 2000;
57(1):
90 - 93.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bergeron, T. M. Meyer, J. T. Coyle, and R. W. Greene
Modulation of N-methyl-D-aspartate receptor function by glycine transport
PNAS,
December 22, 1998;
95(26):
15730 - 15734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Danysz and C. G. Parsons
Glycine and N-Methyl-D-Aspartate Receptors: Physiological Significance and Possible Therapeutic Applications
Pharmacol. Rev.,
December 1, 1998;
50(4):
597 - 664.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Borowsky and B. J. Hoffman
Analysis of a Gene Encoding Two Glycine Transporter Variants Reveals Alternative Promoter Usage and a Novel Gene Structure
J. Biol. Chem.,
October 30, 1998;
273(44):
29077 - 29085.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. PALACIN, R. ESTEVEZ, J. BERTRAN, and A. ZORZANO
Molecular Biology of Mammalian Plasma Membrane Amino Acid Transporters
Physiol Rev,
October 1, 1998;
78(4):
969 - 1054.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. W. Chan and P. E. Sawchenko
Organization and Transmitter Specificity of Medullary Neurons Activated by Sustained Hypertension: Implications for Understanding Baroreceptor Reflex Circuitry
J. Neurosci.,
January 1, 1998;
18(1):
371 - 387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Supplisson and C. Bergman
Control of NMDA Receptor Activation by a Glycine Transporter Co-Expressed in Xenopus Oocytes
J. Neurosci.,
June 15, 1997;
17(12):
4580 - 4590.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Olivares, C. Aragon, C. Gimenez, and F. Zafra
Analysis of the Transmembrane Topology of the Glycine Transporter GLYT1
J. Biol. Chem.,
January 10, 1997;
272(2):
1211 - 1217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Horiuchi, A. Nicke, J. Gomeza, A. Aschrafi, G. Schmalzing, and H. Betz
Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopus oocytes
PNAS,
February 13, 2001;
98(4):
1448 - 1453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
