 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 2948-2960, Copyright © 1995 by Society for Neuroscience
Relative densities of synaptic and extrasynaptic GABAA receptors on cerebellar granule cells as determined by a quantitative immunogold method
Z Nusser, JD Roberts, A Baude, JG Richards and P Somogyi
Medical Research Council, Anatomical Neuropharmacology Unit, University of Oxford, United Kingdom.
Ion channels gated by the inhibitory neurotransmitter gamma- aminobutyric
acid (GABA) are thought to be located in synaptic junctions, but they have
also been found throughout the somatodendritic membrane of neurons
independent of synapses. To test whether synaptic junctions are enriched in
GABAA receptors, and to determine the relative densities of synaptic and
extrasynaptic receptors, the alpha 1 and beta 2/3 subunits of the GABAA
receptor were localized on cerebellar granule cells using a postembedding
immunogold method in cats. Immunoparticle density for the alpha 1 and beta
2/3 subunits was approximately 230 and 180 times more concentrated,
respectively, in the synaptic junction made by GABAergic Golgi cell
terminals with granule cell dendrites than on the extrasynaptic somatic
membrane. Quantification of immunoreactivity revealed one synapse
population for the beta 2/3, but appeared to show two populations for the
alpha 1 subunit immunoreactivity. The concentration of these subunits on
somatic membrane was significantly lower than on the extrasynaptic
dendritic membrane. Synaptic junctions with glutamatergic mossy fiber
terminals were immunonegative. The results demonstrate that granule cells
receiving GABAergic synapses at a restricted location on their distal
dendrites exhibit a highly compartmentalized distribution of GABAA receptor
in their plasma membrane.
This article has been cited by other articles:
|
|
|
|
 
C. J. Herden, N. E. Pardo, R. K. Hajela, Y. Yuan, and W. D. Atchison
Differential Effects of Methylmercury on {gamma}-Aminobutyric Acid Type A Receptor Currents in Rat Cerebellar Granule and Cerebral Cortical Neurons in Culture
J. Pharmacol. Exp. Ther.,
February 1, 2008;
324(2):
517 - 528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Liang, A. Suryanarayanan, A. Abriam, B. Snyder, R. W. Olsen, and I. Spigelman
Mechanisms of Reversible GABAA Receptor Plasticity after Ethanol Intoxication
J. Neurosci.,
November 7, 2007;
27(45):
12367 - 12377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Baude, C. Bleasdale, Y. Dalezios, P. Somogyi, and T. Klausberger
Immunoreactivity for the GABAA Receptor {alpha}1 Subunit, Somatostatin and Connexin36 Distinguishes Axoaxonic, Basket, and Bistratified Interneurons of the Rat Hippocampus
Cereb Cortex,
September 1, 2007;
17(9):
2094 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Kinney
GAT-3 Transporters Regulate Inhibition in the Neocortex
J Neurophysiol,
December 1, 2005;
94(6):
4533 - 4537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Keros and J. J. Hablitz
Subtype-Specific GABA Transporter Antagonists Synergistically Modulate Phasic and Tonic GABAA Conductances in Rat Neocortex
J Neurophysiol,
September 1, 2005;
94(3):
2073 - 2085.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Petrini, I. Marchionni, P. Zacchi, W. Sieghart, and E. Cherubini
Clustering of Extrasynaptic GABAA Receptors Modulates Tonic Inhibition in Cultured Hippocampal Neurons
J. Biol. Chem.,
October 29, 2004;
279(44):
45833 - 45843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Liang, E. Cagetti, R. W. Olsen, and I. Spigelman
Altered Pharmacology of Synaptic and Extrasynaptic GABAA Receptors on CA1 Hippocampal Neurons Is Consistent with Subunit Changes in a Model of Alcohol Withdrawal and Dependence
J. Pharmacol. Exp. Ther.,
September 1, 2004;
310(3):
1234 - 1245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Dibbens, H.-J. Feng, M. C. Richards, L. A. Harkin, B. L. Hodgson, D. Scott, M. Jenkins, S. Petrou, G. R. Sutherland, I. E. Scheffer, et al.
GABRD encoding a protein for extra- or peri-synaptic GABAA receptors is a susceptibility locus for generalized epilepsies
Hum. Mol. Genet.,
July 1, 2004;
13(13):
1315 - 1319.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Zhang, T. Pangrsic, M. Kreft, M. Krzan, N. Li, J.-Y. Sul, M. Halassa, E. Van Bockstaele, R. Zorec, and P. G. Haydon
Fusion-related Release of Glutamate from Astrocytes
J. Biol. Chem.,
March 26, 2004;
279(13):
12724 - 12733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Klausberger, J. D. B. Roberts, and P. Somogyi
Cell Type- and Input-Specific Differences in the Number and Subtypes of Synaptic GABAA Receptors in the Hippocampus
J. Neurosci.,
April 1, 2002;
22(7):
2513 - 2521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mohler, J. M. Fritschy, and U. Rudolph
A New Benzodiazepine Pharmacology
J. Pharmacol. Exp. Ther.,
January 1, 2002;
300(1):
2 - 8.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Z. Kieval, G. W. Hubert, A. Charara, J.-F. Pare, and Y. Smith
Subcellular and Subsynaptic Localization of Presynaptic and Postsynaptic Kainate Receptor Subunits in the Monkey Striatum
J. Neurosci.,
November 15, 2001;
21(22):
8746 - 8757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Scotti and H. Reuter
Synaptic and extrasynaptic gamma -aminobutyric acid type A receptor clusters in rat hippocampal cultures during development
PNAS,
March 1, 2001;
(2001)
61028798.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. F. Hainfeld and R. D. Powell
New Frontiers in Gold Labeling
J. Histochem. Cytochem.,
April 1, 2000;
48(4):
471 - 480.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Racca, F. A. Stephenson, P. Streit, J. D. B. Roberts, and P. Somogyi
NMDA Receptor Content of Synapses in Stratum Radiatum of the Hippocampal CA1 Area
J. Neurosci.,
April 1, 2000;
20(7):
2512 - 2522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. Banks and R. A. Pearce
Kinetic Differences between Synaptic and Extrasynaptic GABAA Receptors in CA1 Pyramidal Cells
J. Neurosci.,
February 1, 2000;
20(3):
937 - 948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bai, P. S. Pennefather, J. F. MacDonald, and B. A. Orser
The General Anesthetic Propofol Slows Deactivation and Desensitization of GABAA Receptors
J. Neurosci.,
December 15, 1999;
19(24):
10635 - 10646.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Chery and Y. De Koninck
Junctional versus Extrajunctional Glycine and GABAA Receptor-Mediated IPSCs in Identified Lamina I Neurons of the Adult Rat Spinal Cord
J. Neurosci.,
September 1, 1999;
19(17):
7342 - 7355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. G. Brickley, S. G. Cull-Candy, and M. Farrant
Single-Channel Properties of Synaptic and Extrasynaptic GABAA Receptors Suggest Differential Targeting of Receptor Subtypes
J. Neurosci.,
April 15, 1999;
19(8):
2960 - 2973.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Maex and E. D. Schutter
Synchronization of Golgi and Granule Cell Firing in a Detailed Network Model of the Cerebellar Granule Cell Layer
J Neurophysiol,
November 1, 1998;
80(5):
2521 - 2537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Nusser, W. Sieghart, and P. Somogyi
Segregation of Different GABAA Receptors to Synaptic and Extrasynaptic Membranes of Cerebellar Granule Cells
J. Neurosci.,
March 1, 1998;
18(5):
1693 - 1703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Nirenberg, J. Chan, A. Pohorille, R. A. Vaughan, G. R. Uhl, M. J. Kuhar, and V. M. Pickel
The Dopamine Transporter: Comparative Ultrastructure of Dopaminergic Axons in Limbic and Motor Compartments of the Nucleus Accumbens
J. Neurosci.,
September 15, 1997;
17(18):
6899 - 6907.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Jones, E. R. Korpi, R. M. McKernan, R. Pelz, Z. Nusser, R. Makela, J. R. Mellor, S. Pollard, S. Bahn, F. A. Stephenson, et al.
Ligand-Gated Ion Channel Subunit Partnerships: GABAA Receptor alpha 6 Subunit Gene Inactivation Inhibits delta Subunit Expression
J. Neurosci.,
February 15, 1997;
17(4):
1350 - 1362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Van Bockstaele, E. E. O. Colago, P. Cheng, A. Moriwaki, G. R. Uhl, and V. M. Pickel
Ultrastructural Evidence for Prominent Distribution of the µ-Opioid Receptor at Extrasynaptic Sites on Noradrenergic Dendrites in the Rat Nucleus Locus Coeruleus
J. Neurosci.,
August 15, 1996;
16(16):
5037 - 5048.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Svingos, A. Moriwaki, J. B. Wang, G. R. Uhl, and V. M. Pickel
Ultrastructural Immunocytochemical Localization of µ-Opioid Receptors in Rat Nucleus Accumbens: Extrasynaptic Plasmalemmal Distribution and Association with Leu5-Enkephalin
J. Neurosci.,
July 1, 1996;
16(13):
4162 - 4173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tia, J. F. Wang, N. Kotchabhakdi, and S. Vicini
Developmental Changes of Inhibitory Synaptic Currents in Cerebellar Granule Neurons: Role of GABAA Receptor alpha 6 Subunit
J. Neurosci.,
June 1, 1996;
16(11):
3630 - 3640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Popratiloff, R. J. Weinberg, and A. Rustioni
AMPA Receptor Subunits Underlying Terminals of Fine-Caliber Primary Afferent Fibers
J. Neurosci.,
May 15, 1996;
16(10):
3363 - 3372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Schaerer, K. Kannenberg, P. Hunziker, S. W. Baumann, and E. Sigel
Interaction between GABAA Receptor beta Subunits and the Multifunctional Protein gC1q-R
J. Biol. Chem.,
July 6, 2001;
276(28):
26597 - 26604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Scotti and H. Reuter
Synaptic and extrasynaptic gamma -aminobutyric acid type A receptor clusters in rat hippocampal cultures during development
PNAS,
March 13, 2001;
98(6):
3489 - 3494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
