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Journal of Neuroscience, Vol 15, 2995-3012, Copyright © 1995 by Society for Neuroscience
Pharmacological dissection of multiple types of Ca2+ channel currents in rat cerebellar granule neurons
A Randall and RW Tsien
Department of Molecular and Cellular Physiology, Beckman Center, Stanford University Medical Center, California 94305, USA.
The diversity of Ca2+ channel types in rat cerebellar granule neurons was
investigated with whole-cell recordings (5 mM external Ba2+). Contributions
of five different high-voltage-activated Ca2+ channel current components
were distinguished pharmacologically. Nimodipine- sensitive L-type current
and omega-CTx-GVIA-sensitive N-type current contributed 15 and 20% of the
total current, respectively. The bulk of the remaining current (46%) was
inhibited by omega-Aga-IVA. The current blocked by this toxin was further
subdivided into two components, P- type and Q-type, on the basis of
differences in their inactivation kinetics and sensitivity to
omega-Aga-IVA. P-Type current was noninactivating during 0.1 sec
depolarizations, half-blocked at about 1- 3 nM omega-Aga-IVA, and
contributed approximately 11% of the total current; Q-type current was
prominently inactivating, half-blocked at approximately 90 nM
omega-Aga-IVA, and comprised 35% of the total current. Both P- and Q-type
currents were potently inhibited by the Conus magus toxin omega-CTx-MVIIC.
A current component resistant to all of the aforementioned blockers
(R-type) displayed more rapid inactivation than the other components and
constituted 19% of the total current. The Q-type current, the largest of
the current components in the granule neurons, resembles currents that can
be generated in Xenopus oocytes by expression of cloned alpha 1A subunits.
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|
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|
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|
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|
|
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|
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|
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|
|
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|
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[PDF]
|
|
|
|
|
|
|
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5623 - 5631.
[Abstract]
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|
|
|
|
|
|
|
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May 15, 2004;
557(1):
159 - 174.
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145(5):
2375 - 2383.
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|
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91(3):
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[Abstract]
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|
|
|
|
|
|
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December 1, 2003;
55(4):
607 - 627.
[Abstract]
[Full Text]
[PDF]
|
|
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[Abstract]
[Full Text]
[PDF]
|
|
|
|
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[Abstract]
[Full Text]
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|
|
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23(17):
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[PDF]
|
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100(6):
3491 - 3496.
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|
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C. T. Tamse, Y. Xu, H. Song, L. Nie, and E. N. Yamoah
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89(3):
1718 - 1726.
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|
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|
|
|
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J. Neurosci.,
March 1, 2003;
23(5):
1593 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
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K. L. Moulder, R. J. Cormier, A. A. Shute, C. F. Zorumski, and S. Mennerick
Homeostatic Effects of Depolarization on Ca2+ Influx, Synaptic Signaling, and Survival
J. Neurosci.,
March 1, 2003;
23(5):
1825 - 1831.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Peng, R. K. Hajela, and W. D. Atchison
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302(2):
424 - 432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
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|
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277(16):
13856 - 13862.
[Abstract]
[Full Text]
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|
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22(6):
2023 - 2034.
[Abstract]
[Full Text]
[PDF]
|
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119(4):
313 - 328.
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277(9):
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