 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 3053-3064, Copyright © 1995 by Society for Neuroscience
The activation of protein kinase A pathway selectively inhibits anterograde axonal transport of vesicles but not mitochondria transport or retrograde transport in vivo
Y Okada, R Sato-Yoshitake and N Hirokawa
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan.
To shed light on how axonal transport is regulated, we examined the
possible roles of protein kinase A (PKA) in vivo suggested by our previous
work (Sato-Yoshitake et al., 1992). Pharmacological probes or the purified
catalytic subunit of PKA were applied to the permeabilized- reactivated
model of crayfish walking leg giant axon, and the effect was monitored by
the quantitative video-enhanced light microscopy and the quantitative
electron microscopy. Dibutyryl cyclic AMP caused concentration-dependent
transient reduction in the number of anterogradely transported small
vesicles, while the retrogradely transported organelles and anterogradely
transported mitochondria showed no decrease. This transient selective
inhibition of anterograde vesicle transport was reversed by the application
of a specific inhibitor of PKA (KT5720) in a concentration-dependent
manner, and was reproduced by the application of the purified catalytic
subunit of PKA and augmented by the application of adenosine
5'-O-(3-thiotriphosphate) (ATP gamma S). Corresponding biochemical study
showed that several axoplasmic proteins including kinesin were specifically
phosphorylated by the activation of the PKA pathway. These findings suggest
the possible roles of PKA in the regulation of the direction of the axonal
transport in vivo. The finding that only vesicle transport but not
mitochondria transport was inhibited also suggests that the transport of
vesicles and that of mitochondria are differently regulated and might be
supported by different motors.
This article has been cited by other articles:
|
|
|
|
 
P. J. Hollenbeck and W. M. Saxton
The axonal transport of mitochondria
J. Cell Sci.,
December 1, 2005;
118(23):
5411 - 5419.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Malaiyandi, A. S. Honick, G. L. Rintoul, Q. J. Wang, and I. J. Reynolds
Zn2+ Inhibits Mitochondrial Movement in Neurons by Phosphatidylinositol 3-Kinase Activation
J. Neurosci.,
October 12, 2005;
25(41):
9507 - 9514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Santama, C. P. N. Er, L.-L. Ong, and H. Yu
Distribution and functions of kinectin isoforms
J. Cell Sci.,
September 1, 2004;
117(19):
4537 - 4549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Miller and M. P. Sheetz
Axonal mitochondrial transport and potential are correlated
J. Cell Sci.,
June 1, 2004;
117(13):
2791 - 2804.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Nybakken, C. W. Turck, D. J. Robbins, and J. M. Bishop
Hedgehog-stimulated Phosphorylation of the Kinesin-related Protein Costal2 Is Mediated by the Serine/Threonine Kinase Fused
J. Biol. Chem.,
June 28, 2002;
277(27):
24638 - 24647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Reese and L. T. Haimo
Dynein, Dynactin, and Kinesin II's Interaction with Microtubules Is Regulated during Bidirectional Organelle Transport
J. Cell Biol.,
October 3, 2000;
151(1):
155 - 166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-Y. Tsai, G. Morfini, G. Szebenyi, and S. T. Brady
Release of Kinesin from Vesicles by hsc70 and Regulation of Fast Axonal Transport
Mol. Biol. Cell,
June 1, 2000;
11(6):
2161 - 2173.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Takeda, H. Yamazaki, D.-H. Seog, Y. Kanai, S. Terada, and N. Hirokawa
Kinesin Superfamily Protein 3 (KIF3) Motor Transports Fodrin-associating Vesicles Important for Neurite Building
J. Cell Biol.,
March 20, 2000;
148(6):
1255 - 1266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okada and N. Hirokawa
Mechanism of the single-headed processivity: Diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin
PNAS,
January 18, 2000;
97(2):
640 - 645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dorner, A. Ullrich, H.-U. Haring, and R. Lammers
The Kinesin-like Motor Protein KIF1C Occurs in Intact Cells as a Dimer and Associates with Proteins of the 14-3-3 Family
J. Biol. Chem.,
November 19, 1999;
274(47):
33654 - 33660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dorner, T. Ciossek, S. Muller, N. P. H. Moller, A. Ullrich, and R. Lammers
Characterization of KIF1C, a New Kinesin-like Protein Involved in Vesicle Transport from the Golgi Apparatus to the Endoplasmic Reticulum
J. Biol. Chem.,
August 7, 1998;
273(32):
20267 - 20275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Kim, Y. Tang, Y. Okada, T. A. Torrey, S. K. Chattopadhyay, M. Pfleiderer, F. G. Falkner, F. Dorner, W. Choi, N. Hirokawa, et al.
Binding of Murine Leukemia Virus Gag Polyproteins to KIF4, a Microtubule-Based Motor Protein
J. Virol.,
August 1, 1998;
72(8):
6898 - 6901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Gindhart Jr., C. J. Desai, S. Beushausen, K. Zinn, and L. S.B. Goldstein
Kinesin Light Chains Are Essential for Axonal Transport in Drosophila
J. Cell Biol.,
April 20, 1998;
141(2):
443 - 454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hirokawa
Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport
Science,
January 23, 1998;
279(5350):
519 - 526.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J.-C. Larcher, D. Boucher, S. Lazereg, F. Gros, and P. Denoulet
Interaction of Kinesin Motor Domains with alpha - and beta -Tubulin Subunits at a Tau-independent Binding Site. REGULATION BY POLYGLUTAMYLATION
J. Biol. Chem.,
September 6, 1996;
271(36):
22117 - 22124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
