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Journal of Neuroscience, Vol 15, 4289-4297, Copyright © 1995 by Society for Neuroscience
Effects of estradiol and progesterone on voltage-gated calcium and potassium conductances in rat CA1 hippocampal neurons
M Joels and H Karst
Institute for Neurobiology, Amsterdam Graduate School Neurosciences, Amsterdam, The Netherlands.
In this study we recorded voltage gated Ca and K conductances with patch
electrodes in hippocampal slices from female rats, which were
ovariectomized (OVX) 1 week before the experiment. One experimental group
was primed with estradiol at day 3 and 4 after OVX and received
progesterone 4 hr before the start of the experiment. The second group was
treated with estradiol at day 3 and 4, but received vehicle at the day of
the experiment. The third group was not treated with hormones (only vehicle
injections). We observed that the amplitude of both sustained and transient
Ca currents were significantly enhanced in CA1 pyramidal neurons recorded
in tissue from estradiol primed rats receiving progesterone when compared
to the nontreated OVX controls; without estradiol priming Ca current
amplitudes were small. Current amplitudes in tissue from rats treated with
estradiol only, were at intermediate values. The voltage for half-maximal
steady state inactivation of the transient Ca current was at a less
negative value for the estradiol/progesterone treated group in comparison
to the OVX controls. Input resistances of the cells, and voltage or kinetic
properties of the currents as recorded under these conditions were
comparable for the three groups. Characteristics of two voltage gated K
conductances, that is, the transient IA and the delayed rectifier,
displayed no consistent differences for the three experimental groups. We
conclude that long-term modulation of estradiol and progesterone levels
alters the Ca but not the K currents that were tested in CA1 pyramidal
neurons. These alterations may be linked to steroid-induced morphological
changes in dendrites of CA1 pyramidal cells.
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