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Journal of Neuroscience, Vol 15, 4382-4394, Copyright © 1995 by Society for Neuroscience
Neuroblast long-term cell cultures from human fetal olfactory epithelium respond to odors
GB Vannelli, F Ensoli, R Zonefrati, Y Kubota, A Arcangeli, A Becchetti, G Camici, T Barni, CJ Thiele and GC Balboni
Department of Human Anatomy and Histology, University of Florence, Italy.
Primary cell cultures from human fetal olfactory neuroepithelium have been
isolated, cloned, and propagated in continuous in vitro culture for
approximately 1 year. The two clones we report here synthesize both
neuronal proteins and olfactory-specific markers as well as the putative
olfactory neurotransmitter, carnosine. In addition, patchclamp experiments
reveal that these cells are electrically excitable. Following exposure to a
panel of aromatic chemicals one of the cell cultures shows a specific
increase in intracellular cAMP, indicating that some degree of functional
maturity is expressed in vitro. The results suggest that these cells
originate from the "stem cell" compartment that gives rise to mature
olfactory receptor neurons. These long-term cell cultures represent models
that will be useful in studying the mechanism(s) of olfaction and the
regulation of olfactory neurogenesis and differentiation.
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