 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 4592-4601, Copyright © 1995 by Society for Neuroscience
Evidence for glycine modulation of excitatory synaptic inputs to retinal ganglion cells
PD Lukasiewicz and RC Roeder
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110-1093, USA.
The actions of glycine on the NMDA receptor-mediated synaptic responses of
ganglion cells were studied in the tiger salamander retinal slice. Ganglion
cell excitatory postsynaptic currents (EPSCs) were elicited either by
exciting bipolar cells with potassium puffs or by light stimulation, and
were measured using whole-cell patch-clamp techniques. Increasing bath
glycine concentrations to 10 microM had little effect on the amplitude of
the puff-evoked EPSCs, indicating either that synaptic glycine
concentrations were saturating or that the added glycine was buffered by
uptake mechanisms. However, 5,7- dichlorokynurenic acid (5,7-DCK), an
antagonist for the glycine site on the NMDA receptor, reduced the ganglion
cell responses to NMDA puffs, and reduced the potassium puff- and
light-elicited EPSCs. The IC50 values for 5,7-DCK became larger with
increasing glycine concentrations, but not with increasing NMDA
concentrations, indicating that 5,7-DCK acted at the glycine site. The IC50
values for 5,7-DCK were increased with stronger potassium puffs or light
stimuli, suggesting that synaptic glycine levels increased with the
strength of the stimuli. EPSCs measured in ON-OFF ganglion cells at light
ON and OFF were reduced by 5,7-DCK. For dim light stimuli, the IC50 values
were lower for the OFF EPSCs compared to the ON EPSCs, indicating that
glycine concentrations were different at the times of OFF and ON activity.
Estimates of synaptic glycine concentrations suggest that for dim light
stimuli, concentrations at the OFF synapses were not saturated, but
concentrations at the ON synapses were saturated.
This article has been cited by other articles:
|
|
|
|
 
T. Schubert, D. Kerschensteiner, E. D. Eggers, T. Misgeld, M. Kerschensteiner, J. W. Lichtman, P. D. Lukasiewicz, and R. O. L. Wong
Development of Presynaptic Inhibition Onto Retinal Bipolar Cell Axon Terminals Is Subclass-Specific
J Neurophysiol,
July 1, 2008;
100(1):
304 - 316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Gregg, M. Kamermans, J. Klooster, P. D. Lukasiewicz, N. S. Peachey, K. A. Vessey, and M. A. McCall
Nyctalopin Expression in Retinal Bipolar Cells Restores Visual Function in a Mouse Model of Complete X-Linked Congenital Stationary Night Blindness
J Neurophysiol,
November 1, 2007;
98(5):
3023 - 3033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Eggers, M. A. McCall, and P. D. Lukasiewicz
Presynaptic inhibition differentially shapes transmission in distinct circuits in the mouse retina
J. Physiol.,
July 15, 2007;
582(2):
569 - 582.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Gustafson, E. R. Stevens, H. Wolosker, and R. F. Miller
Endogenous D-Serine Contributes to NMDA-Receptor-Mediated Light-Evoked Responses in the Vertebrate Retina
J Neurophysiol,
July 1, 2007;
98(1):
122 - 130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Eggers and P. D. Lukasiewicz
Receptor and transmitter release properties set the time course of retinal inhibition.
J. Neurosci.,
September 13, 2006;
26(37):
9413 - 9425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Eggers and P. D. Lukasiewicz
GABAA, GABAC and glycine receptor-mediated inhibition differentially affects light-evoked signalling from mouse retinal rod bipolar cells
J. Physiol.,
April 1, 2006;
572(1):
215 - 225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lamas, I. Lee-Rivera, and A. M. Lopez-Colome
Cell-specific Expression of N-Methyl-D-Aspartate Receptor Subunits in Muller Glia and Neurons from the Chick Retina
Invest. Ophthalmol. Vis. Sci.,
October 1, 2005;
46(10):
3570 - 3577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. R. Stevens, M. Esguerra, P. M. Kim, E. A. Newman, S. H. Snyder, K. R. Zahs, and R. F. Miller
D-serine and serine racemase are present in the vertebrate retina and contribute to the physiological activation of NMDA receptors
PNAS,
May 27, 2003;
100(11):
6789 - 6794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. Shields and P. D. Lukasiewicz
Spike-Dependent GABA Inputs to Bipolar Cell Axon Terminals Contribute to Lateral Inhibition of Retinal Ganglion Cells
J Neurophysiol,
May 1, 2003;
89(5):
2449 - 2458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. McCall, P. D. Lukasiewicz, R. G. Gregg, and N. S. Peachey
Elimination of the rho 1 Subunit Abolishes GABAC Receptor Expression and Alters Visual Processing in the Mouse Retina
J. Neurosci.,
May 15, 2002;
22(10):
4163 - 4174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chen and J. S. Diamond
Synaptically Released Glutamate Activates Extrasynaptic NMDA Receptors on Cells in the Ganglion Cell Layer of Rat Retina
J. Neurosci.,
March 15, 2002;
22(6):
2165 - 2173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. Shields, M. N. Tran, R. O. L. Wong, and P. D. Lukasiewicz
Distinct Ionotropic GABA Receptors Mediate Presynaptic and Postsynaptic Inhibition in Retinal Bipolar Cells
J. Neurosci.,
April 1, 2000;
20(7):
2673 - 2682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. T. Wong, K. L. Myhr, E. D. Miller, and R. O. L. Wong
Developmental Changes in the Neurotransmitter Regulation of Correlated Spontaneous Retinal Activity
J. Neurosci.,
January 1, 2000;
20(1):
351 - 360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. H. Higgs and P. D. Lukasiewicz
Glutamate Uptake Limits Synaptic Excitation of Retinal Ganglion Cells
J. Neurosci.,
May 15, 1999;
19(10):
3691 - 3700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Berger, S. Dieudonne, and P. Ascher
Glycine Uptake Governs Glycine Site Occupancy at NMDA Receptors of Excitatory Synapses
J Neurophysiol,
December 1, 1998;
80(6):
3336 - 3340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Lukasiewicz and C. R. Shields
Different Combinations of GABAA and GABAC Receptors Confer Distinct Temporal Properties to Retinal Synaptic Responses
J Neurophysiol,
June 1, 1998;
79(6):
3157 - 3167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. F. Fischer, P. D. Lukasiewicz, and R. O. L. Wong
Age-Dependent and Cell Class-Specific Modulation of Retinal Ganglion Cell Bursting Activity by GABA
J. Neurosci.,
May 15, 1998;
18(10):
3767 - 3778.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Lukasiewicz, J. A. Wilson, and J. E. Lawrence
AMPA-Preferring Receptors Mediate Excitatory Synaptic Inputs to Retinal Ganglion Cells
J Neurophysiol,
January 1, 1997;
77(1):
57 - 64.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
