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Journal of Neuroscience, Vol 15, 4906-4917, Copyright © 1995 by Society for Neuroscience
Cellular localization of synaptotagmin I, II, and III mRNAs in the central nervous system and pituitary and adrenal glands of the rat
B Marqueze, JA Boudier, M Mizuta, N Inagaki, S Seino and M Seagar
INSERM U 374, Institut Jean Roche, Faculte de Medecine-Nord, Marseille, France.
Three isoforms of synaptotagmin, a synaptic vesicle protein involved in
neurotransmitter release, have been characterized in the rat, although
functional differences between these isoforms have not been reported. In
situ hybridization was used to define the localization of synaptotagmin I,
II, and III transcripts in the rat CNS and pituitary and adrenal glands.
Each of the three synaptotagmin genes has a unique expression pattern. The
synaptotagmin III gene is expressed in most neurons, but transcripts are
much less abundant than the products of the synaptotagmin I and II genes. A
majority of neurons in the forebrain expressed both synaptotagmin I and III
mRNAs while synaptotagmin II gene expression was confined to subsets of
neurons in layers IV-VI of the cerebral cortex, in the dentate granule cell
region, the hilus, and the CA1-CA3 areas of the hippocampus. In the
cerebellum, all three transcripts were visualized in the granule cell
layer. Furthermore, synaptotagmin I probes revealed striking differences
between distinct populations of neurons, as in addition to moderate
labeling of granule cells, much more prominent hybridization signals were
detected on scattered cell bodies likely to be Golgi interneurons. In the
most caudal part of the brain, synaptotagmin II transcripts were abundant
and were coexpressed with synaptotagmin III mRNAs. This pattern was found
in putative motoneurons of the spinal cord, suggesting that the two
isoforms might be involved in exocytosis at the neuromuscular junction.
Only synaptotagmin I mRNAs were detected in the anterior and intermediate
pituitary and in adrenal medullary cells. These data reveal an unexpectedly
subtle segregation of the expression of synaptotagmin genes and the
existence of multiple combinations of synaptotagmin isoforms which may
provide diversity in the regulation of neurosecretion.
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