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Journal of Neuroscience, Vol 15, 4918-4926, Copyright © 1995 by Society for Neuroscience
Nerve growth factor (NGF) differentially regulates the chemosensitivity of adult rat cultured sensory neurons
S Bevan and J Winter
Sandoz Institute for Medical Research, London, United Kingdom.
We have studied the effects of NGF on the chemosensitivity of adult rat DRG
neurons over a 1-2 week period in vitro, using voltage-clamp and
radioactive ion flux methods. A sustained proton evoked current was
reversibly lost in NGF-free medium after 1 week. Proton-evoked efflux of
radioactive 86Rb+ ions was also depressed in NGF deprived cultures,
although depolarization with 40 mM potassium still evoked a large 86Rb+
efflux. A similar reversible loss of capsaicin sensitivity was noted. The
response to GABA and a second, transient proton evoked current were also
regulated by NGF, but over a longer time course. In contrast, the
sensitivity to ATP was not influenced by the presence or absence of NGF.
These data show that NGF regulates some, but not all, chemosensitivities of
DRG neurons and that loss of sensitivity occurs at different rates for
different agonists. The precise co-regulation of the response to capsaicin
and the sustained response to protons provides further evidence that
protons activate capsaicin-operated ion channels.
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