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Journal of Neuroscience, Vol 15, 5004-5013, Copyright © 1995 by Society for Neuroscience
The Shaker-like potassium channels of the mouse rod bipolar cell and their contributions to the membrane current
DJ Klumpp, EJ Song, S Ito, MH Sheng, LY Jan and LH Pinto
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA.
RT PCR on mRNA from enzymatically dissociated, isolated bipolar cells
showed that these neurons express the Shaker-like K+ channels Kv1.1, Kv1.2,
and Kv1.3. Immunohistochemical localization showed each channel to have a
unique subcellular distribution: Kv1.1 immunoreactivity was detected in the
dendrites and axons terminal, whereas Kv1.2 and Kv1.3 subunits were
localized to the axon and the postsynaptic membrane of the rod ribbon
synapse, respectively. Whole-cell patch-clamp recordings indicated that the
activation voltage of the delayed rectifier current of the isolated bipolar
cell and the inhibitory constants for current blockade by TEA, 4-AP, and
Ba2+ were similar to these same properties measured for Kv1.1 expressed in
oocytes. However, the TEA and 4-AP inhibitory constants for the bipolar
cell current differed from the inhibitory constants for Kv1.2 or Kv1.3.
These results suggest that the current of the isolated rod bipolar cell is
most similar to Kv1.1 but that all three channels may function in the
intact retina to allow complex modulation of retinal synaptic signals.
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