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Journal of Neuroscience, Vol 15, 5222-5237, Copyright © 1995 by Society for Neuroscience
Electron microscopic analysis of D1 and D2 dopamine receptor proteins in the dorsal striatum and their synaptic relationships with motor corticostriatal afferents
SM Hersch, BJ Ciliax, CA Gutekunst, HD Rees, CJ Heilman, KK Yung, JP Bolam, E Ince, H Yi and AI Levey
Department of Neurology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
The precise localization of D1 and D2 dopamine receptors within striatal
neurons and circuits is crucial information for further understanding
dopamine pharmacology. We have used subtype specific polyclonal and
monoclonal antibodies against D1 and D2 dopamine receptors to determine
their cellular and subcellular distributions, their colocalization, and
their differential connectivity with motor cortical afferents labeled
either by lesion-induced degeneration or by anterograde transport of
biotinylated dextrans. D1 and D2 are primarily expressed in medium-sized
neurons and spiny dendrites. Axon terminals containing D1 were rare whereas
D2-immunoreactive axon terminals forming symmetrical synapses with
dendrites and spines were common. In 2 microns sections, D1 was localized
to 53% of neurons, and D2 to 48% of neurons, while mixing D1 and D2
antibodies labeled 78%. By electron microscopy, D1 was localized to 43% of
dendrites and 38% of spines while D2 was localized to 38% of dendrites and
48% of spines. Combining D1 and D2 antibodies resulted in the labeling of
88.5% of dendrites and 92.6% of spines. Using different chromogens for D1
and D2, colocalization was not observed. Ipsilateral motor corticostriatal
afferents were primarily axospinous and significantly more synapsed with D1
than D2-positive spines (65% vs 47%). Contralateral motor corticostriatal
afferents were frequently axodendritic and no difference in their frequency
of synapses with D1 and D2 dendrites and spines was observed. These
findings demonstrate differential patterns of expression of D1 and D2
receptors in striatal neurons and axon terminals and their differential
involvement in motor corticostriatal circuits.
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