 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 5681-5692, Copyright © 1995 by Society for Neuroscience
Temperature compensation and temperature entrainment of the chick pineal cell circadian clock
RK Barrett and JS Takahashi
NSF Center for Biological Timing, Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA.
We have used an in vitro model system of the circadian clock, dispersed
chick pineal cells, to examine the effects of temperature on the circadian
clock of a homeotherm. This preparation enabled us to isolate a circadian
clock from in vivo homeostatic temperature regulation and expose cells to
both constant temperatures and abrupt temperature changes. By manipulating
the temperature of the pineal cells, we have demonstrated that (1) the
circadian clock compensates its period for temperature changes over the
range of 34-40 degrees C; Q10 = 0.83, a value within the range of Q10
values measured for poikilothermic circadian clocks; (2) temperature pulses
(42 degrees C, 6 hr duration) shift the phase (advance and delay) of the
circadian rhythm in a phase- dependent manner; and (3) a temperature cycle
(18 hr at 37 degrees C, 6 hr at 42 degrees C) will entrain the circadian
clock in vitro. This is the first demonstration of temperature entrainment
of the circadian clock of a homeotherm in vitro. In addition we have found
that temperature directly influences the synthesis and release of
melatonin, the primary hormonal product of the pineal gland. The
biosynthesis of melatonin is strongly temperature dependent with a Q10 >
11 when melatonin release is measured at ambient temperatures between 31
degrees C and 40 degrees C. In contrast, 6 hr 42 degrees C temperatures
pulses acutely inhibit melatonin release in a manner similar to that seen
previously with light pulses. These results demonstrate that a circadian
clock from a homeothermic vertebrate is temperature compensated, yet
temperature cycles can entrain the circadian melatonin rhythm. Thus, the
chick pineal circadian oscillator has retained all the fundamental
properties of circadian rhythms.
This article has been cited by other articles:
|
|
|
|
 
M. Izumo, C. H. Johnson, and S. Yamazaki
Circadian gene expression in mammalian fibroblasts revealed by real-time luminescence reporting: Temperature compensation and damping
PNAS,
December 23, 2003;
100(26):
16089 - 16094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Herzog and R. M. Huckfeldt
Circadian Entrainment to Temperature, But Not Light, in the Isolated Suprachiasmatic Nucleus
J Neurophysiol,
August 1, 2003;
90(2):
763 - 770.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hayashi, K. Sanada, T. Hirota, F. Shimizu, and Y. Fukada
p38 Mitogen-activated Protein Kinase Regulates Oscillation of Chick Pineal Circadian Clock
J. Biol. Chem.,
June 27, 2003;
278(27):
25166 - 25171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Burgoon and J. A. Boulant
Temperature-sensitive properties of rat suprachiasmatic nucleus neurons
Am J Physiol Regulatory Integrative Comp Physiol,
September 1, 2001;
281(3):
R706 - R715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. Holtzclaw
Circadian Rhythmicity and Homeostatic Stability in Thermoregulation
Biol Res Nurs,
April 1, 2001;
2(4):
221 - 235.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. D. McCormick, S. Moriyama, and B. T. Bjornsson
Low temperature limits photoperiod control of smolting in Atlantic salmon through endocrine mechanisms
Am J Physiol Regulatory Integrative Comp Physiol,
May 1, 2000;
278(5):
R1352 - R1361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. T. Firth, I. Belan, D. J. Kennaway, and R. W. Moyer
Thermocyclic entrainment of lizard blood plasma melatonin rhythms in constant and cyclic photic environments
Am J Physiol Regulatory Integrative Comp Physiol,
December 1, 1999;
277(6):
R1620 - R1626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. F. Ruby, D. E. Burns, and H. C. Heller
Circadian Rhythms in the Suprachiasmatic Nucleus are Temperature-Compensated and Phase-Shifted by Heat Pulses In Vitro
J. Neurosci.,
October 1, 1999;
19(19):
8630 - 8636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Liu, M. Merrow, J. J. Loros, and J. C. Dunlap
How Temperature Changes Reset a Circadian Oscillator
Science,
August 7, 1998;
281(5378):
825 - 829.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Tosini and M. Menaker
Multioscillatory Circadian Organization in a Vertebrate, Iguana iguana
J. Neurosci.,
February 1, 1998;
18(3):
1105 - 1114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Barrett and J. S. Takahashi
Lability of Circadian Pacemaker Amplitude in Chick Pineal Cells: A Temperature-Dependent Process
J Biol Rhythms,
August 1, 1997;
12(4):
309 - 318.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. J. Weitz
Circadian timekeeping: Loops and layers of transcriptional control
PNAS,
December 10, 1996;
93(25):
14308 - 14309.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Florez and J. S. Takahashi
Quantitative Two-Dimensional Gel Electrophoretic Analysis of Clock-Controlled Proteins in Cultured Chick Pineal Cells: Circadian Regulation of Tryptophan Hydroxylase
J Biol Rhythms,
September 1, 1996;
11(3):
241 - 257.
[Abstract]
[PDF]
|
|
|
|
|
|
|
N. F. Ruby and H. C. Heller
Temperature Sensitivity of the Suprachiasmatic Nucleus of Ground Squirrels and Rats in vitro
J Biol Rhythms,
June 1, 1996;
11(2):
126 - 136.
[Abstract]
[PDF]
|
|
|
|
|
|
|
N. W. Chong, M. Bernard, and D. C. Klein
Characterization of the Chicken Serotonin N-Acetyltransferase Gene. ACTIVATION VIA CLOCK GENE HETERODIMER/E BOX INTERACTION
J. Biol. Chem.,
October 13, 2000;
275(42):
32991 - 32998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
