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Journal of Neuroscience, Vol 15, 5693-5702, Copyright © 1995 by Society for Neuroscience
Pre- and postsynaptic determinants of EPSC waveform at cerebellar climbing fiber and parallel fiber to Purkinje cell synapses
M Takahashi, Y Kovalchuk and D Attwell
Department of Physiology, University College London, England.
Excitatory postsynaptic currents (EPSCs) at the parallel fiber and climbing
fiber to Purkinje cell synapses were studied by whole-cell clamping
Purkinje cells in cerebellar slices. Reducing glutamate release with
adenosine or GABA decreased the amplitude of the EPSCs, with a larger
suppression being produced at the parallel fiber synapse. Reducing
glutamate release also speeded the decay of the EPSCs, and this effect was
not a series resistance artefact since postsynaptic reduction of the
current with CNQX did not speed the EPSC decay. Blocking glutamate uptake
slowed the decay of the EPSCs. At the climbing fiber synapse, adenosine had
little suppressive effect on the smaller EPSC evoked by the second of a
pair of stimuli. Blocking desensitization of postsynaptic AMPA receptors
prolonged the EPSC decay, preferentially increased the size of the second
EPSC, and resulted in adenosine having a similar suppressive effect on the
first and second EPSC. These data suggest that, at these synapses, the fall
of glutamate concentration in the synaptic cleft overlaps with the decay of
the EPSC, and that the EPSC size and duration are controlled by the amount
of glutamate released, the rate of glutamate uptake, and desensitization.
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