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Journal of Neuroscience, Vol 15, 6085-6093, Copyright © 1995 by Society for Neuroscience
Resolution and pharmacological analysis of the voltage-dependent calcium channels of Drosophila larval muscles
ML Gielow, GG Gu and S Singh
Department of Biochemical Pharmacology, State University of New York at Buffalo 14260, USA.
Voltage-dependent calcium channels play a role in many cellular phenomena.
Very little is known about Ca2+ channels in Drosophila, especially those in
muscles. Existing literature on neuronal Ca2+ channels of Drosophila
suggests that their pharmacology may be distinct from that of vertebrate
Ca2+ channels. This raises questions on the pharmacology and diversity of
Ca2+ channels in Drosophila muscles. Here we show that the Ca2+ channel
current in the body-wall muscles of Drosophila larvae consists of two main
components. One component is sensitive to 1,4-dihydropyridines and
diltiazem, which block vertebrate L-type Ca2+ channels. The second
component is sensitive to amiloride, which blocks vertebrate T-type Ca2+
channels. In contrast to Drosophila brain membrane preparations in which a
majority of the Ca2+ channels are phenylalkylamine-sensitive but
dihydropyridine-insensitive, the major current in the muscles was
dihydropyridine-sensitive but relatively less sensitive to verapamil. This
might indicate an underlying tissue specific distribution of distinct
subtypes of dihydropyridine/phenylalkylamine-sensitive Ca2+ channels in
Drosophila. Low verapamil sensitivity of the dihydropyridine-sensitive
current of Drosophila muscles also set it apart from the vertebrate L-type
channels which are sensitive to 1,4-dihydropyridines, benzothiazepines as
well as phenylalkylamines. The dihydropyridine-sensitive current in
Drosophila muscles activated in a similar voltage range as the vertebrate
L-type current. As with the vertebrate current, blockade by
dihydropyridines was voltage dependent.(ABSTRACT TRUNCATED AT 250 WORDS)
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