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Journal of Neuroscience, Vol 16, 298-306, Copyright © 1996 by Society for Neuroscience
The synaptic drive from the spinal locomotor network to motoneurons in the newborn rat
JR Cazalets, M Borde and F Clarac
CNRS, NBM, Marseille, France.
The nature of the synaptic drive from the locomotor spinal network onto the
motoneurons was studied in the newborn rat. For this purpose, an in vitro
isolated spinal cord preparation of newborn rat was used. The recording
chamber was partitioned with Vaseline walls to separate the L1/L2 lumbar
segments, in which the spinal locomotor network is located, from the
motoneurons in the lower lumbar segments. Locomoter- like activity was
induced by bath-applying a mixture of serotonin and NMDA to segments L1/L2.
In this way, the synaptic activity could be modified at the lower lumbar
level without affecting the motor pattern. The drive elicited onto the
motoneurons during sequences of locomoter- like activity, which was
monitored by performing intracellular recordings, consisting of an
inhibitory component followed by an excitatory component. The inhibitory
synaptic volley was reversed at a membrane potential of --60 mV with K
acetate electrodes, whereas it was shifted toward positive values with KCl
electrodes. The glycinergic blocker strychnine, bath-applied to segments
L3/L5, blocked the inhibitory drive without affecting the rhythmic
activity, whereas it disrupted the locomoter-like activity when
bath-applied to segments L1/L2. The inhibitory part of the drive was more
sensitive than the excitatory part to changes in the membrane potential.
The excitatory phase was mixed and consisted of an NMDA and a non-NMDA
component, which were sensitive to 2-amino-5-phosphonovaleric acid and
6-cyano-7- nitroquinoxaline-2,3-dione, respectively. It was concluded that
the locomotor network located in segments L1/L2 sends a biphasic projection
to the various groups of motoneurons located along the lumbar spinal cord.
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