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Journal of Neuroscience, Vol 16, 46-54, Copyright © 1996 by Society for Neuroscience
Calcium-independent activation of the secretory apparatus by ruthenium red in hippocampal neurons: a new tool to assess modulation of presynaptic function
LE Trudeau, RT Doyle, DG Emery and PG Haydon
Department of Zoology and Genetics, Iowa State University, Ames 50011, USA.
The functional plasticity of the nervous system may result in part from the
direct modulation of the effectiveness of the release machinery of synaptic
terminals. To date, direct modulation of secretion in neurons has proven
difficult to study because of the lack of a suitable tool to probe the
release machinery independently of calcium influx. We report that the
polyvalent cation ruthenium red (RR) directly evokes rapid and reversible
calcium-independent quantal secretion in hippocampal neurons by binding to
external sites on the presynaptic terminal membrane. This binding can be
displaced by heparin and is not associated with ultrastructural damage to
the synaptic terminals. The use of RR-evoked release as a tool has allowed
us to detect a direct modulation of the secretory apparatus after
activation of A1 adenosine receptors on hippocampal neurons.
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