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Volume 16, Number 10, Issue of May 15, 1996 pp. 3123-3129
Copyright ©1996 Society for Neuroscience

Naturally Occurring Truncated trkB Receptors Have Dominant Inhibitory Effects on Brain-Derived Neurotrophic Factor Signaling

Received Oct. 17, 1995; revised Feb. 7, 1996; accepted Feb. 19, 1996.

Fernette F. Eide1, Ella R. Vining2, Brock L. Eide3, Keling Zang2, Xiao-Yun Wang2, and Louis F. Reichardt2, 4

Department of 1 Neurology, The University of Chicago, Chicago, Illinois 60637, and 2 Department of Physiology, 3 Cardiovascular Research Institute, and 4 The Howard Hughes Medical Institute, University of California, San Francisco, California 94143-0724

trkB encodes a receptor tyrosine kinase activated by three neurotrophins---brain-derived neurotrophic factor (BDNF), neurotrophin-3, and neurotrophin-4/5. In vivo, three isoforms of the receptor are generated by differential splicing---gp145trkB or the full-length trkB receptor, and trkB.T1 and trkB.T2, two cytoplasmically truncated receptors that lack kinases, but contain unique C termini. Although the truncated receptors appear to be precisely regulated during nervous system development and regeneration, their role in neurotrophin signaling has not been directly tested. In this paper, we studied the signaling properties and interactions of gp145trkB, trkB.T1, and trkB.T2 by expressing the receptors in a Xenopus oocyte microinjection assay. We found that oocytes expressing gp145trkB, but not trkB.T1 or trkB.T2, were capable of eliciting 45Ca efflux responses (a phospholipase C-gamma -mediated mechanism) after stimulation by BDNF. When trkB.T1 and trkB.T2 were coexpressed with gp145trkB, they acted as dominant negative receptors, inhibiting the BDNF signal by forming nonfunctional heterodimers with the full-length receptors. An ATP-binding mutant of gp145trkB had similar dominant inhibitory effects. Our data suggest that naturally occurring truncated trkB receptors function as inhibitory modulators of neurotrophin responsiveness. Furthermore, the homodimerization of gp145trkB appears to be an essential step in activation of the BDNF signaling cascade.

Key words: BDNF; dominant negative; neurotrophin; truncated trkB; tyrosine kinase; Xenopus oocyte




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