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Volume 16, Number 10, Issue of May 15, 1996 pp. 3247-3255
Copyright ©1996 Society for NeuroscienceInfluences of the Thalamus on the Survival of Subplate and Cortical Plate Cells in Cultured Embryonic Mouse Brain
Received Dec. 11, 1995; revised Feb. 26, 1996; accepted Feb. 28, 1996.
David J. Price and R. Beau Lotto Department of Physiology, University Medical School, Edinburgh EH8 9AG, United Kingdom
The afferent and efferent connections of the cerebral neocortex develop simultaneously toward the end of embryogenesis. At this stage, the neocortex comprises two main cell-dense layers: the thicker and more superficial cortical plate (future layers 2-6) and the thinner underlying subplate. Many early thalamocortical projections temporarily innervate the subplate before leaving it to locate their ultimate targets in the overlying cortical plate. The subplate then disappears. In this study, we performed in vitro experiments on late embryonic murine brain to test whether the thalamus can influence the survival of cortical plate and subplate cells at this stage. In isolated organotypic cortical explants from embryonic day 19 mice, most of the cells that had formed the subplate died. Coculture with a thalamic explant prevented this loss; coculture with additional cortical or cerebellar explants did not. By contrast, many cells in or destined for the cortical plate survived even in isolated cortical explants; coculture with a thalamic explant did not alter the numbers of these cells that survived. Our results suggest that the thalamus provides trophic support for subplate cells but not for late embryonic cortical plate cells. In vivo, a loss of thalamic-derived trophic support for the subplate late in embryogenesis, consequent on the movement of thalamocortical axons into the cortical plate, may contribute to subplate death.
Key words: cortical plate; corticothalamic projection; growth factors; mouse; organotypic culture; subplate; thalamocortical projection; trophism
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