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Volume 16, Number 11, Issue of June 1, 1996 pp. 3590-3600
Copyright ©1996 Society for Neuroscience

Predominant Expression of Platelet-Activating Factor Receptor in the Rat Brain Microglia

Received Nov. 3, 1995; revised March 13, 1996; accepted March 15, 1996.

Mikiro Mori1, Makoto Aihara1, Kazuhiko Kume1, Makoto Hamanoue2, Shinichi Kohsaka2, and Takao Shimizu1

1 Department of Biochemistry, Faculty of Medicine, The University of Tokyo, Tokyo 113, Japan, and 2 Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo 187, Japan

Cellular localization of platelet-activating factor (PAF) receptor in the rat brain was determined by (1) in situ hybridization, (2) Northern blot analysis in primary cell cultures of neurons, microglia, astrocytes, and fibroblasts, and (3) Ca2+ imaging in hippocampal culture. In situ hybridization revealed that the PAF receptor mRNA is expressed intensely in microglia and moderately in neurons. Northern blot analysis revealed that PAF receptor mRNA is the most abundant in microglia. In primary hippocampal cultures, PAF elevated intracellular Ca2+ concentration in microglia and also in neurons, but to a lesser extent. These results suggest predominant presence of PAF receptor in microglia. Cultured microglia also expressed cPLA2 mRNA the most intensely. PAF-activated microglia released arachidonic acid in a Ca2+-dependent manner and without conversion to its derivatives. We propose that microglia as well as neurons contribute to PAF-related events in the CNS by releasing arachidonic acid.

Key words: platelet-activating factor; PAF; receptor; mRNA; microglia; in situ hybridization; Ca2+ imaging; arachidonic acid




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