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Volume 16, Number 11, Issue of June 1, 1996 pp. 3661-3671
Copyright ©1996 Society for Neuroscience

Identification and Characterization of a Ca²⁺-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons

Received Aug. 28, 1995; revised March 13, 1996; accepted March 15, 1996.

Gisela F. Wilson¹, Frank C. Richardson¹, Thomas E. Fisher¹, Baldomero M. Olivera², and Leonard K. Kaczmarek¹

¹ Department of Pharmacology, Yale University, New Haven, Connecticut 06510, and ² Department of Biology, University of Utah, Salt Lake City, Utah 84112

The afterdischarge of Aplysia bag cell neurons has served as a model system for the study of phosphorylation-mediated changes in neuronal excitability. The nature of the depolarization generating the afterdischarge, however, has remained unclear. We now have found that venom from Conus textile triggers a similar prolonged discharge, and we have identified a slow inward current and corresponding channel, the activation of which seems to contribute to the onset of the discharge. The slow inward current is voltage-dependent and Ca²⁺-sensitive, reverses at potentials slightly positive to 0 mV, exhibits a selectivity of K  Na  Tris > N-methyl-D-glucamine (NMDG), and is blocked by high concentrations of tetrodotoxin. Comparison of these features with those observed in channel recordings provides evidence that a Ca²⁺-sensitive, nonspecific cation channel is responsible for a slow inward current that regulates spontaneous repetitive firing and suggests that modulation of the cation channel underlies prolonged changes in neuronal response properties.

Key words: Ca²⁺-activated nonspecific cation channel; slow inward current; ion channel modulation; afterdischarge; bursting; Aplysia bag cell neurons; Conus textile

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