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Volume 16, Number 11, Issue of June 1, 1996 pp. 3727-3736
Copyright ©1996 Society for Neuroscience

Dopamine Receptor Agonists Regulate Levels of the Serotonin 5-HT_2A Receptor and its mRNA in a Subpopulation of Rat Striatal Neurons

Received Jan. 19, 1996; revised March 6, 1996; accepted March 8, 1996.

Nathalie Laprade¹, Fatiha Radja², Tomás A. Reader^{2, 3}, and Jean-Jacques Soghomonian¹

¹ Centre de Recherche en Neurobiologie and Département d'Anatomie, Faculté de Médecine, Université Laval, Québec, Canada, and Départements de ² Physiologie et ³ Psychiatrie, Faculté de Médecine, Université de Montréal, Québec, Canada

The effects of dopamine receptor agonists on the levels of the striatal serotonin 5-HT_2A receptor and its mRNA were investigated in rats lesioned with 6-OHDA as neonates. The mRNA encoding for the 5-HT_2A receptor was detected by in situ hybridization histochemistry and the binding to 5-HT_2A receptors was revealed with [¹²⁵I](2,5-dimethoxy-4-iodophenyl)2-aminopropane ([¹²⁵I]DOI). In adult control unlesioned rats, labeling with the 5-HT_2A cRNA probe and with [¹²⁵I]DOI was concentrated in medial sectors of the striatum. In 6-OHDA-lesioned rats, labeling with the 5-HT_2A cRNA probe or with [¹²⁵I]DOI was increased in the striatum, particularly in its lateral subdivisions. These increases were abolished after chronic systemic administration of the dopamine receptor agonists apomorphine or SKF-38393. The mRNA levels encoding for the 5-HT_2A receptor were further measured in individual striatal neurons after double-labeling of sections with a 5-HT_2A and a preproenkephalin (PPE) cRNA probe. In control unlesioned rats, 5-HT_2A mRNA labeling was distributed in PPE-labeled as well as in PPE-unlabeled striatal neurons. In 6-OHDA-lesioned rats, increased 5-HT_2A mRNA labeling was found only in PPE-unlabeled neurons and it was abolished after apomorphine or SKF-38393 administration. These results demonstrate that agonists of dopamine receptors inhibit the expression of 5-HT_2A receptors in a subpopulation of presumed striato-nigral neurons. We propose that this regulation plays an important role in the control of motor activity by dopamine and 5-HT in the basal ganglia.

Key words: striatum; 5-HT; dopamine; 6-OHDA; 5-HT_2A serotonin receptor; D1 dopamine receptor

This article has been cited by other articles:

				C. Bishop, J. L. Tessmer, T. Ullrich, K. C. Rice, and P. D. Walker Serotonin 5-HT2A Receptors Underlie Increased Motor Behaviors Induced in Dopamine-Depleted Rats by Intrastriatal 5-HT2A/2C Agonism J. Pharmacol. Exp. Ther., August 1, 2004; 310(2): 687 - 694. [Abstract] [Full Text] [PDF]

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