QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (76) Citing Articles via Google Scholar Google Scholar Articles by Feng, G. Articles by Hall, L. M. Search for Related Content PubMed PubMed Citation Articles by Feng, G. Articles by Hall, L. M. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL

 Previous Article  |  Next Article 

Volume 16, Number 12, Issue of June 15, 1996 pp. 3925-3933
Copyright ©1996 Society for Neuroscience

Cloning and Functional Characterization of a Novel Dopamine Receptor from Drosophila melanogaster

Received Nov. 30, 1995; revised March 26, 1996; accepted April 2, 1996.

Guoping Feng¹, Frances Hannan^{1, 2}, Vincenzina Reale², Yuen Yi Hon¹, Christopher T. Kousky¹, Peter D. Evans², and Linda M. Hall¹

¹ Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, New York 14260-1200, and ² The Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom

A cDNA clone is described that encodes a novel G-protein-coupled dopamine receptor (DopR99B) expressed in Drosophila heads. The DopR99B receptor maps to 99B3-5, close to the position of the octopamine/tyramine receptor gene at 99A10-B1, suggesting that the two may be related through a gene duplication. Agonist stimulation of DopR99B receptors expressed in Xenopus oocytes increased intracellular Ca²⁺ levels monitored as changes in an endogenous inward Ca²⁺-dependent chloride current. In addition to initiating this intracellular Ca²⁺ signal, stimulation of DopR99B increased cAMP levels. The rank order of potency of agonists in stimulating the chloride current is: dopamine > norepinephrine > epinephrine > tyramine. Octopamine and 5-hydroxytryptamine are not active (<100 µM). This pharmacological profile plus the second-messenger coupling pattern suggest that the DopR99B receptor is a D1-like dopamine receptor. However, the hydrophobic core region of the DopR99B receptor shows almost equal amino acid sequence identity (40-48%) with vertebrate serotonergic, 1- and -adrenergic, and D1-like and D2-like dopaminergic receptors. Thus, this Drosophila receptor defines a novel structural class of dopamine receptors. Because DopR99B is the second dopamine receptor cloned from Drosophila, this work establishes dopamine receptor diversity in a system amenable to genetic dissection.

Key words: cloned dopamine receptor; Drosophila melanogaster; Xenopus oocyte expression; adenylyl cyclase; calcium; gene mapping

This article has been cited by other articles:

				M. C. Clark, T. E. Dever, J. J. Dever, P. Xu, V. Rehder, M. A. Sosa, and D. J. Baro Arthropod 5-HT2 Receptors: A Neurohormonal Receptor in Decapod Crustaceans That Displays Agonist Independent Activity Resulting from an Evolutionary Alteration to the DRY Motif J. Neurosci., March 31, 2004; 24(13): 3421 - 3435. [Abstract] [Full Text] [PDF]

				T. Farooqui, K. Robinson, H. Vaessin, and B. H. Smith Modulation of Early Olfactory Processing by an Octopaminergic Reinforcement Pathway in the Honeybee J. Neurosci., June 15, 2003; 23(12): 5370 - 5380. [Abstract] [Full Text] [PDF]

				M. G. Hearn, Y. Ren, E. W. McBride, I. Reveillaud, M. Beinborn, and A. S. Kopin A Drosophila dopamine 2-like receptor: Molecular characterization and identification of multiple alternatively spliced variants PNAS, October 29, 2002; 99(22): 14554 - 14559. [Abstract] [Full Text] [PDF]

				T. Brody and A. Cravchik Drosophila melanogasterG Protein-Coupled Receptors J. Cell Biol., July 24, 2000; 150(2): 83 - 88. [Full Text] [PDF]

				R. Andretic and J. Hirsh Circadian modulation of dopamine receptor responsiveness in Drosophila melanogaster PNAS, February 15, 2000; 97(4): 1873 - 1878. [Abstract] [Full Text] [PDF]

				S. Chyb, W. Hevers, M. Forte, W. J. Wolfgang, Z. Selinger, and R. C. Hardie Modulation of the Light Response by cAMP in Drosophila Photoreceptors J. Neurosci., October 15, 1999; 19(20): 8799 - 8807. [Abstract] [Full Text] [PDF]

				L. G. Hammerland, J. E. Garrett, B. C. P. Hung, C. Levinthal, and E. F. Nemeth Allosteric Activation of the Ca2+ Receptor Expressed in Xenopus laevis Oocytes by NPS 467 or NPS 568 Mol. Pharmacol., June 1, 1998; 53(6): 1083 - 1088. [Abstract] [Full Text]

				W. S. Neckameyer Dopamine and Mushroom Bodies in Drosophila: Experience-Dependent and -Independent Aspects of Sexual Behavior Learn. Mem., May 1, 1998; 5(1): 157 - 165. [Abstract] [Full Text]

				V. Reale, F. Hannan, L. M. Hall, and P. D. Evans Agonist-Specific Coupling of a Cloned Drosophila melanogaster D1-Like Dopamine Receptor to Multiple Second Messenger Pathways by Synthetic Agonists J. Neurosci., September 1, 1997; 17(17): 6545 - 6553. [Abstract] [Full Text] [PDF]

				S. M. Nicola and R. C. Malenka Dopamine Depresses Excitatory and Inhibitory Synaptic Transmission by Distinct Mechanisms in the Nucleus Accumbens J. Neurosci., August 1, 1997; 17(15): 5697 - 5710. [Abstract] [Full Text] [PDF]

				C. Yellman, H. Tao, B. He, and J. Hirsh Conserved and sexually dimorphic behavioral responses to biogenic amines in decapitated Drosophila PNAS, April 15, 1997; 94(8): 4131 - 4136. [Abstract] [Full Text] [PDF]

				T. Tully, G. Bolwig, J. Christensen, J. Connolly, M. DelVecchio, J. DeZazzo, J. Dubnau, C. Jones, S. Pinto, M. Regulski, et al. A Return to Genetic Dissection of Memory in Drosophila Cold Spring Harb Symp Quant Biol, January 1, 1996; 61(0): 207 - 218. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help