WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Discover www.zeiss.de/functionality
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (134)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cadas, H.
Right arrow Articles by Piomelli, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cadas, H.
Right arrow Articles by Piomelli, D.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*2-AMINOETHANOL
*CALCIUM COMPOUNDS
*CALCIUM, ELEMENTAL
*CARBACHOL CHLORIDE
*TRITIUM
*VERATRIDINE

 Previous Article  |  Next Article 

Volume 16, Number 12, Issue of June 15, 1996 pp. 3934-3942
Copyright ©1996 Society for Neuroscience

Biosynthesis of an Endogenous Cannabinoid Precursor in Neurons and its Control by Calcium and cAMP

Received Feb. 14, 1996; revised March 27, 1996; accepted April 1, 1996.

Hugues Cadas1, Sylvie Gaillet2, Massimiliano Beltramo1, Laurent Venance2, and Daniele Piomelli1

1 The Neurosciences Institute, San Diego, California 92121, and 2 Institut National de la Santé et de la Recherche Médicale, Paris, France

Understanding the mechanisms involved in the biogenesis of N-arachidonoylethanolamine (anandamide) and Npalmitoylethanolamine is important in view of the possible role of these lipids as endogenous cannabinoid substances. Anandamide (which activates cannabinoid CB1 receptors) and N-palmitoylethanolamine (which activates a CB2-like receptor subtype in mast cells) may both derive from cleavage of precursor phospholipid, N-acylphosphatidylethanolamine (NAPE), catalyzed by Ca2+-activated D-type phosphodiesterase activity. We report here that the de novo biosynthesis of NAPE is enhanced in a Ca2+-dependent manner when rat cortical neurons are stimulated with the Ca2+-ionophore ionomycin or with membrane-depolarizing agents such as veratridine and kainate. This reaction is likely to be mediated by a neuronal N-acyltransferase activity, which catalyzes the transfer of an acyl group from phosphatidylcholine to the ethanolamine moiety of phosphatidylethanolamine. In addition, we show that Ca2+-dependent NAPE biosynthesis is potentiated by agents that increase cAMP levels, including forskolin and vasoactive intestinal peptide. Our results thus indicate that NAPE levels in cortical neurons are controlled by Ca2+ ions and cAMP. Such regulatory effect may participate in maintaining a supply of cannabimimetic N-acylethanolamines during synaptic activity, and prime target neurons for release of these bioactive lipids.

Key words: anandamide; N-acylethanolamines; phosphatidylethanolamine; N-acylphosphatidylethanolamine; N-acyltransferase; vasoactive intestinal peptide; endogenous cannabinoids




This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
G. Astarita, F. Ahmed, and D. Piomelli
Identification of biosynthetic precursors for the endocannabinoid anandamide in the rat brain
J. Lipid Res., January 1, 2008; 49(1): 48 - 57.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
I. KYROU, G. VALSAMAKIS, and C. TSIGOS
The Endocannabinoid System as a Target for the Treatment of Visceral Obesity and Metabolic Syndrome
Ann. N.Y. Acad. Sci., November 1, 2006; 1083(1): 270 - 305.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. LoVerme, M. Guzman, S. Gaetani, and D. Piomelli
Cold Exposure Stimulates Synthesis of the Bioactive Lipid Oleoylethanolamide in Rat Adipose Tissue
J. Biol. Chem., August 11, 2006; 281(32): 22815 - 22818.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Malcher-Lopes, S. Di, V. S. Marcheselli, F.-J. Weng, C. T. Stuart, N. G. Bazan, and J. G. Tasker
Opposing crosstalk between leptin and glucocorticoids rapidly modulates synaptic excitation via endocannabinoid release.
J. Neurosci., June 14, 2006; 26(24): 6643 - 6650.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
L. G. Bilsland, J. R. T. Dick, G. Pryce, S. Petrosino, V. Di Marzo, D. Baker, and L. Greensmith
Increasing cannabinoid levels by pharmacological and genetic manipulation delay disease progression in SOD1 mice
FASEB J, May 1, 2006; 20(7): 1003 - 1005.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. Astarita, B. C. Rourke, J. B. Andersen, J. Fu, J. H. Kim, A. F. Bennett, J. W. Hicks, and D. Piomelli
Postprandial increase of oleoylethanolamide mobilization in small intestine of the Burmese python (Python molurus)
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1407 - R1412.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. A. Moore, G. G. Nomikos, A. K. Dickason-Chesterfield, D. A. Schober, J. M. Schaus, B.-P. Ying, Y.-C. Xu, L. Phebus, R. M. A. Simmons, D. Li, et al.
From The Cover: Identification of a high-affinity binding site involved in the transport of endocannabinoids
PNAS, December 6, 2005; 102(49): 17852 - 17857.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Gonzalez-Yanes, A. Serrano, F. J. Bermudez-Silva, M. Hernandez-Dominguez, M. A. Paez-Ochoa, F. R. de Fonseca, and V. Sanchez-Margalet
Oleylethanolamide impairs glucose tolerance and inhibits insulin-stimulated glucose uptake in rat adipocytes through p38 and JNK MAPK pathways
Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E923 - E929.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Tian, J. Guo, F. Yao, D.-P. Yang, and A. Makriyannis
The Conformation, Location, and Dynamic Properties of the Endocannabinoid Ligand Anandamide in a Membrane Bilayer
J. Biol. Chem., August 19, 2005; 280(33): 29788 - 29795.
[Abstract] [Full Text] [PDF]

Home page
 Alcohol AlcoholHome page
F. RODRIGUEZ de FONSECA, I. DEL ARCO, F. J. BERMUDEZ-SILVA, A. BILBAO, A. CIPPITELLI, and M. NAVARRO
THE ENDOCANNABINOID SYSTEM: PHYSIOLOGY AND PHARMACOLOGY
Alcohol Alcohol., January 1, 2005; 40(1): 2 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. Ronesi and D. M. Lovinger
Induction of striatal long-term synaptic depression by moderate frequency activation of cortical afferents in rat
J. Physiol., January 1, 2005; 562(1): 245 - 256.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. C. Azad, K. Monory, G. Marsicano, B. F. Cravatt, B. Lutz, W. Zieglgansberger, and G. Rammes
Circuitry for Associative Plasticity in the Amygdala Involves Endocannabinoid Signaling
J. Neurosci., November 3, 2004; 24(44): 9953 - 9961.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
G. Marsicano, S. Goodenough, K. Monory, H. Hermann, M. Eder, A. Cannich, S. C. Azad, M. G. Cascio, S. O. Gutierrez, M. van der Stelt, et al.
CB1 Cannabinoid Receptors and On-Demand Defense Against Excitotoxicity
Science, October 3, 2003; 302(5642): 84 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
T. F. FREUND, I. KATONA, and D. PIOMELLI
Role of Endogenous Cannabinoids in Synaptic Signaling
Physiol Rev, July 1, 2003; 83(3): 1017 - 1066.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
W.-R. Schabitz, A. Giuffrida, C. Berger, A. Aschoff, M. Schwaninger, S. Schwab, and D. Piomelli
Release of Fatty Acid Amides in a Patient With Hemispheric Stroke: A Microdialysis Study
Stroke, August 1, 2002; 33(8): 2112 - 2114.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. A. Day, F. Rakhshan, D. G. Deutsch, and E. L. Barker
Role of Fatty Acid Amide Hydrolase in the Transport of the Endogenous Cannabinoid Anandamide
Mol. Pharmacol., June 1, 2001; 59(6): 1369 - 1375.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
J. J. Rodriguez, K. Mackie, and V. M. Pickel
Ultrastructural Localization of the CB1 Cannabinoid Receptor in {micro}-Opioid Receptor Patches of the Rat Caudate Putamen Nucleus
J. Neurosci., February 1, 2001; 21(3): 823 - 833.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Siman, A. G. Reaume, M. J. Savage, S. Trusko, Y.-G. Lin, R. W. Scott, and D. G. Flood
Presenilin-1 P264L Knock-In Mutation: Differential Effects on Abeta Production, Amyloid Deposition, and Neuronal Vulnerability
J. Neurosci., December 1, 2000; 20(23): 8717 - 8726.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Beltramo, N. Stella, A. Calignano, S. Y. Lin, A. Makriyannis, and D. Piomelli
Functional Role of High-Affinity Anandamide Transport, as Revealed by Selective Inhibition
Science, August 22, 1997; 277(5329): 1094 - 1097.
[Abstract] [Full Text]

Home page
 BMJHome page
M. A Schuckit
Science, medicine, and the future: Substance use disorders
BMJ, May 31, 1997; 314(7094): 1605 - 1605.
[Full Text]

Home page
 J. Neurosci.Home page
H. Cadas, E. di Tomaso, and D. Piomelli
Occurrence and Biosynthesis of Endogenous Cannabinoid Precursor, N-Arachidonoyl Phosphatidylethanolamine, in Rat Brain
J. Neurosci., February 15, 1997; 17(4): 1226 - 1242.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Siman, D. G. Flood, G. Thinakaran, and R. W. Neumar
Endoplasmic Reticulum Stress-induced Cysteine Protease Activation in Cortical Neurons. EFFECT OF AN ALZHEIMER'S DISEASE-LINKED PRESENILIN-1 KNOCK-IN MUTATION
J. Biol. Chem., November 21, 2001; 276(48): 44736 - 44743.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-