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Volume 16, Number 14, Issue of July 15, 1996 pp. 4479-4490
Copyright ©1996 Society for Neuroscience

Immunocytochemical Localization of the GABAC Receptor rho  Subunits in the Mammalian Retina

Received Feb. 16, 1996; revised April 19, 1996; accepted April 24, 1996.

Ralf Enz, Johann Helmut Brandstätter, Heinz Wässle, and Joachim Bormann

Max-Planck-Institut für Hirnforschung, Neuroanatomische Abteilung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany

Polyclonal antibodies against the N terminus of the rat rho 1 subunit were generated to study the distribution of GABAC receptors in the mammalian retina. The specificity of the antibodies was tested in Western blots and transfected HEK-293 cells. No cross-reactivity with the GABAA receptor subunits alpha 1-3, beta 1-3, gamma 2, delta  or with the glycine receptor subunits alpha 1 and beta  could be detected. In contrast, the rho 1, rho 2, and rho 3 subunits were all recognized by the antibodies. In vertical sections of rat, rabbit, cat, and macaque monkey retinae, strong punctate immunoreactivity was present in the inner plexiform layer. Weaker immunoreactivity was also present in the outer plexiform layer, and cell bodies of bipolar cells were faintly labeled. Double immunostaining of vertical sections and immunostaining of dissociated rat retinae showed the punctate immunofluorescence to colocalize with bipolar cell axon terminals. The puncta possibly represent clustering of the rho  subunits at postsynaptic sites.

Key words: GABAC receptor; rho subunits; rat retina; rabbit retina; cat retina; macaque monkey retina; bipolar cell; immuno-cytochemistry; antibodies




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