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Volume 16, Number 14,
Issue of July 15, 1996
pp. 4479-4490
Copyright ©1996 Society for Neuroscience
Immunocytochemical Localization of the GABAC
Receptor Subunits in the Mammalian Retina
Received Feb. 16, 1996; revised April 19, 1996; accepted April 24, 1996.
Ralf Enz,
Johann Helmut Brandstätter,
Heinz Wässle, and
Joachim Bormann
Max-Planck-Institut für Hirnforschung, Neuroanatomische
Abteilung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany
Polyclonal antibodies against the N terminus of the rat 1
subunit were generated to study the distribution of
GABAC receptors in the mammalian retina. The
specificity of the antibodies was tested in Western blots and
transfected HEK-293 cells. No cross-reactivity with the
GABAA receptor subunits 1-3, 1-3, 2,
or with the glycine receptor subunits 1 and could be
detected. In contrast, the 1, 2, and 3 subunits were all
recognized by the antibodies. In vertical sections of rat, rabbit, cat,
and macaque monkey retinae, strong punctate immunoreactivity was
present in the inner plexiform layer. Weaker immunoreactivity was also
present in the outer plexiform layer, and cell bodies of bipolar cells
were faintly labeled. Double immunostaining of vertical sections and
immunostaining of dissociated rat retinae showed the punctate
immunofluorescence to colocalize with bipolar cell axon terminals.
The puncta possibly represent clustering of the subunits at
postsynaptic sites.
Key words:
GABAC receptor;
subunits;
rat retina;
rabbit retina;
cat retina;
macaque monkey retina;
bipolar cell;
immuno-cytochemistry;
antibodies
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