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Volume 16, Number 15, Issue of August 1, 1996 pp. 4579-4587
Copyright ©1996 Society for Neuroscience

The Fibroblast Growth Factor Receptor-1 Is Necessary for the Induction of Neurite Outgrowth in PC12 Cells by aFGF

Received March 6, 1996; revised May 3, 1996; accepted May 14, 1996.

Hsien-Yi Lin1, Jingsong Xu4, David M. Ornitz4, Simon Halegoua3, and Michael J. Hayman2

1 Biochemistry and Molecular Biology Graduate Program, 2 Department of Molecular Genetics and Microbiology, and 3 Department of Neurobiology and Behavior, Institute of Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, and 4 Department of Molecular Biology and Pharmacology, Washington University Medical School, St. Louis, Missouri 63110-8103

The PC12 subclone, fnr-PC12 cells, is defective in neurite outgrowth in response to acidic fibroblast growth factor (aFGF); however, its response to nerve growth factor (NGF) is normal. Examination of the expression of FGF receptors (FGFRs) revealed that although PC12 cells express FGFR-1, -3, and -4, fnr-PC12 cells have a reduced level of expression of FGFR-1 but not FGFR-3 and -4. Transfection of FGFR-1 into fnr-PC12 cells efficiently restored aFGF-induced neurite outgrowth, whereas transfection of FGFR-3 was much less efficient. Transfection of a chimeric receptor consisting of the extracellular domain of FGFR-3 fused to the transmembrane and intracellular domain of FGFR-1, termed FR31b, efficiently restored aFGF-induced neurite outgrowth. This demonstrates that the difference between these two receptors in their ability to induce neurite outgrowth is attributable to differences in the signaling capacity of their cytoplasmic domains. Activation of the chimeric receptor by aFGF induced a stronger and more persistent increase in the tyrosine phosphorylation of cellular proteins than did activation of FGFR-3 alone. In particular, the activation of MAP kinase by FR31b was more persistent than when activated by FGFR-3. This difference in signaling potential of FGFR-1 and -3 in fnr-PC12 cells may account for the difference in the potential for induction of neurite outgrowth. These results demonstrate that FGF-induced neurite outgrowth in PC12 cells occurs mainly via FGFR-1 and not via the other FGFRs expressed in these cells.

Key words: aFGF; FGFR-1; FGFR-3; neurite outgrowth; PC12 cells; fnr-PC12 cells; tyrosine phosphorylation; MAP kinase




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