 |
 Previous Article | Next Article 
Volume 16, Number 15,
Issue of August 1, 1996
pp. 4617-4624
Copyright ©1996 Society for Neuroscience
Selective G-Protein Regulation of Neuronal Calcium Channels
Received Feb. 15, 1996; revised April 18, 1996; accepted May 2, 1996.
Peter T. Toth1,
Lee R. Shekter1,
Gloria Hui
Ma1,
Louis H. Philipson2, and
Richard J. Miller1
1 Department of Pharmacological and Physiological
Sciences, and 2 Department of Medicine, The University of
Chicago, Chicago, Illinois 60637
We examined the properties and regulation of Ca channels resulting
from the expression of human  1B and
1E subunits stably expressed in HEK293 cells.
The ancillary subunits  1B and
2/ were also stably expressed in these cell
lines. Ca currents in 1B-expressing cells had
the properties of N-type currents. Ca currents in cells expressing
1E exhibited a novel profile that was similar
to the properties of the ``R type'' Ca current. Introduction of
GTP- -S into 1B cells greatly enhanced the
extent of prepulse facilitation of the Ca current, whereas it had only
a very small effect in 1E-expressing cells.
Activation of somatostatin receptors endogenous to HEK293 cells or  opioid receptors, expressed in the cells after transfection, inhibited
Ca currents in 1B-expressing cells. This
inhibition was blocked by pertussis toxin and was partially relieved by
a depolarizing prepulse. In contrast, no inhibitory effects were noted
in cells expressing 1E channels under the same
circumstances. HEK293 cells normally contained G-proteins from all of
the four major families. Inhibition of Ca currents by agonists in
1B-expressing cells was enhanced slightly by
the cotransfection of several G-protein subunits. agonists,
however, had no effect in 1E-containing cells,
even after overexpression of different G-protein -subunits. In
summary, these results demonstrate that there is a large difference in
the susceptibility of 1B- and
1E-based Ca channels to regulation by
G-proteins. This is so despite the fact that the two types of Ca
channels show substantial similarities in their primary sequences.
Key words:
Ca channel;
G-protein;
receptor;
patch
clamp;
HEK293;
somatostatin
This article has been cited by other articles:
|
|
|
|
 
H. W. Tedford and G. W. Zamponi
Direct G Protein Modulation of Cav2 Calcium Channels
Pharmacol. Rev.,
December 1, 2006;
58(4):
837 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Li, H. Zhong, T. Scheuer, and W. A. Catterall
Functional Role of a C-Terminal G{beta}{gamma}-Binding Domain of Cav2.2 Channels
Mol. Pharmacol.,
September 1, 2004;
66(3):
761 - 769.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tan, M. Groszer, A. M. Tan, A. Pandya, X. Liu, and C.-W. Xie
Phosphoinositide 3-Kinase Cascade Facilitates {micro}-Opioid Desensitization in Sensory Neurons by Altering G-Protein-Effector Interactions
J. Neurosci.,
November 12, 2003;
23(32):
10292 - 10301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Foehring, P. G. Mermelstein, W.-J. Song, S. Ulrich, and D. J. Surmeier
Unique Properties of R-Type Calcium Currents in Neocortical and Neostriatal Neurons
J Neurophysiol,
November 1, 2000;
84(5):
2225 - 2236.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. R. Mirotznik, X. Zheng, and E. F. Stanley
G-Protein Types Involved in Calcium Channel Inhibition at a Presynaptic Nerve Terminal
J. Neurosci.,
October 15, 2000;
20(20):
7614 - 7621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Simen and R. J. Miller
Involvement of Regions in Domain I in the Opioid Receptor Sensitivity of alpha 1B Ca2+ Channels
Mol. Pharmacol.,
May 1, 2000;
57(5):
1064 - 1074.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. J. Kamp, H. Hu, and E. Marban
Voltage-dependent facilitation of cardiac L-type Ca channels expressed in HEK-293 cells requires beta -subunit
Am J Physiol Heart Circ Physiol,
January 1, 2000;
278(1):
H126 - H136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Canti, K. M. Page, G. J. Stephens, and A. C. Dolphin
Identification of Residues in the N Terminus of alpha 1B Critical for Inhibition of the Voltage-Dependent Calcium Channel by Gbeta gamma
J. Neurosci.,
August 15, 1999;
19(16):
6855 - 6864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Sun and R. J. Miller
Multiple Neuropeptide Y Receptors Regulate K+ and Ca2+ Channels in Acutely Isolated Neurons From the Rat Arcuate Nucleus
J Neurophysiol,
March 1, 1999;
81(3):
1391 - 1403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tanaka, I. Shibuya, N. Kabashima, Y. Ueta, and H. Yamashita
Inhibition of Voltage-Dependent Calcium Channels by Prostaglandin E2 in Rat Melanotrophs
Endocrinology,
December 1, 1998;
139(12):
4801 - 4810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Meza and B. Adams
G-Protein-Dependent Facilitation of Neuronal alpha 1A, alpha 1B, and alpha 1E Ca Channels
J. Neurosci.,
July 15, 1998;
18(14):
5240 - 5252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Page, C. Canti, G. J. Stephens, N. S. Berrow, and A. C. Dolphin
Identification of the Amino Terminus of Neuronal Ca2+ Channel alpha 1 Subunits alpha 1B and alpha 1E as an Essential Determinant of G-Protein Modulation
J. Neurosci.,
July 1, 1998;
18(13):
4815 - 4824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Simen and R. J. Miller
Structural Features Determining Differential Receptor Regulation of Neuronal Ca Channels
J. Neurosci.,
May 15, 1998;
18(10):
3689 - 3698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Zhang and J. T. Schmidt
Adenosine A1 Receptors Mediate Retinotectal Presynaptic Inhibition: Uncoupling by C-Kinase and Role in LTP During Regeneration
J Neurophysiol,
February 1, 1998;
79(2):
501 - 510.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Sun, L. H. Philipson, and R. J. Miller
Regulation of K+ and Ca++ Channels by a Family of Neuropeptide Y Receptors
J. Pharmacol. Exp. Ther.,
February 1, 1998;
284(2):
625 - 632.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. A. McCool, J.-P. Pin, M. M. Harpold, P. F. Brust, K. A. Stauderman, and D. M. Lovinger
Rat group I Metabotropic Glutamate Receptors Inhibit Neuronal Ca2+ Channels via Multiple Signal Transduction Pathways in HEK 293 Cells
J Neurophysiol,
January 1, 1998;
79(1):
379 - 391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chen and N. A. Lambert
Inhibition of Dendritic Calcium Influx by Activation of G-Protein-Coupled Receptors in the Hippocampus
J Neurophysiol,
December 1, 1997;
78(6):
3484 - 3488.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-W. Jeong and R. D. Wurster
Muscarinic Receptor Activation Modulates Ca2+ Channels in Rat Intracardiac Neurons via a PTX- and Voltage-Sensitive Pathway
J Neurophysiol,
September 1, 1997;
78(3):
1476 - 1490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. R. Shekter, R. Taussig, S. E. Gillard, and R. J. Miller
Regulation of Human Neuronal Calcium Channels by G Protein beta gamma Subunits Expressed in Human Embryonic Kidney 293 Cells
Mol. Pharmacol.,
August 1, 1997;
52(2):
282 - 291.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Boehm and H. Betz
Somatostatin Inhibits Excitatory Transmission at Rat Hippocampal Synapses via Presynaptic Receptors
J. Neurosci.,
June 1, 1997;
17(11):
4066 - 4075.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Page, G. J. Stephens, N. S. Berrow, and A. C. Dolphin
The Intracellular Loop between Domains I and II of the B-Type Calcium Channel Confers Aspects of G-Protein Sensitivity to the E-Type Calcium Channel
J. Neurosci.,
February 15, 1997;
17(4):
1330 - 1338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
