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Volume 16, Number 16,
Issue of August 15, 1996
pp. 4846-4860
Copyright ©1996 Society for Neuroscience
Voltage-Gated K+ Channel  Subunits: Expression and
Distribution of Kv1 and Kv2 in Adult Rat Brain
Received Jan. 18, 1996; revised May 9, 1996; accepted May 21, 1996.
Kenneth J. Rhodes1,
Michael M. Monaghan1,
Nestor X. Barrezueta1,
Stanley Nawoschik1,
Zewditu Bekele-Arcuri2,
Maria F. Matos2,
Kensuke Nakahira2,
Lee E. Schechter1, and
James S. Trimmer2
1 CNS Disorders, Wyeth-Ayerst Research, Princeton, New
Jersey 08543, and 2 Department of Biochemistry and Cell
Biology and Institute for Cell and Developmental Biology, State
University of New York, Stony Brook, New York 11794
Recent cloning of K+ channel  subunits
revealed that these cytoplasmic polypeptides can dramatically alter the
kinetics of current inactivation and promote efficient glycosylation
and surface expression of the channel-forming  subunits. Here, we
examined the expression, distribution, and association of two of these
subunits, Kv1 and Kv2, in adult rat brain. In situ
hybridization using cRNA probes revealed that these -subunit genes
are heterogeneously expressed, with high densities of Kv1 mRNA in
the striatum, CA1 subfield of the hippocampus, and cerebellar Purkinje
cells, and high densities of Kv2 mRNA in the cerebral cortex,
cerebellum, and brainstem. Immunohistochemical staining using
subunit-specific monoclonal and affinity-purified polyclonal antibodies
revealed that the Kv1 and Kv2 polypeptides frequently co-localize
and are concentrated in neuronal perikarya, dendrites, and terminal
fields, and in the juxtaparanodal region of myelinated axons.
Immunoblot and reciprocal co-immunoprecipitation analyses indicated
that Kv2 is the major subunit present in rat brain membranes,
and that most K+ channel complexes containing
Kv1 also contain Kv2. Taken together, these data suggest
that Kv2 is a component of almost all K+
channel complexes containing Kv1 subunits, and that individual
channels may contain two or more biochemically and functionally
distinct -subunit polypeptides.
Key words:
ion channel;
central nervous system;
auxiliary subunit;
striatum;
immunoprecipitation;
immunohistochemistry
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