QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Parri, H. R. Articles by Lansman, J. B. Search for Related Content PubMed PubMed Citation Articles by Parri, H. R. Articles by Lansman, J. B.

 Previous Article  |  Next Article 

Volume 16, Number 16, Issue of August 15, 1996 pp. 4890-4902
Copyright ©1996 Society for Neuroscience

Multiple Components of Ca²⁺ Channel Facilitation in Cerebellar Granule Cells: Expression of Facilitation during Development in Culture

Received Oct. 30, 1995; revised May 15, 1996; accepted May 21, 1996.

H. Rheinallt Parri and Jeffry B. Lansman

Department of Cellular and Molecular Pharmacology, School of Medicine, University of California, San Francisco, California 94143-0450

The contribution of pharmacologically distinct Ca²⁺ channels to prepulse-induced facilitation was studied in mouse cerebellar granule cells. Ca²⁺ channel facilitation was measured as the percentage increase in the whole-cell current recorded during a test pulse before and after it was paired with a positive prepulse. The amount of facilitation was small in recordings made during the first few days in tissue culture but increased substantially after 1 week. L-type channels accounted for the largest proportion of facilitation in 1-week-old cells (60-70%), whereas N-type channels contributed very little (~3%). The toxins -agatoxin IVa or -conotoxin MVIIC (after block of N-, L-, and P-type channels) each blocked a small percentage of facilitation (~12 and 14%, respectively). Perfusion of cells with GTP--S enhanced the facilitation of N-type channels, whereas it inhibited facilitation of L-type channels. During development in vitro, the contribution of L-type channels to the whole-cell current decreased. Single-channel recordings showed the presence of 10 and 15 pS L-type Ca²⁺ channels in 1-d-old cells. After 1 week in culture, a ~25 pS L-type channel dominated recordings from cell-attached patches. Positive prepulses increased the activity of the 25 pS channel but not of the smaller conductance channels. The expression of Ca²⁺ channel facilitation during development may contribute to changes in excitability that allow frequency-dependent Ca²⁺ influx during the period of active synaptogenesis.

Key words: calcium channel; calcium; cerebellar granule cell; development; facilitation; L-type channel; N-type channel; P-type channel; Q-type channel; potentiation

This article has been cited by other articles:

				Y. Lu, Y. Lv, Y. Ye, Y. Wang, Y. Hong, M. E. Fortini, Y. Zhong, and Z. Xie A role for presenilin in post-stress regulation: effects of presenilin mutations on Ca2+ currents in Drosophila FASEB J, August 1, 2007; 21(10): 2368 - 2378. [Abstract] [Full Text] [PDF]

				H. Zhou, S.-A. Kim, E. A. Kirk, A. L. Tippens, H. Sun, F. Haeseleer, and A. Lee Ca2+-Binding Protein-1 Facilitates and Forms a Postsynaptic Complex with Cav1.2 (L-Type) Ca2+ Channels J. Neurosci., May 12, 2004; 24(19): 4698 - 4708. [Abstract] [Full Text] [PDF]

				M. Tan, M. Groszer, A. M. Tan, A. Pandya, X. Liu, and C.-W. Xie Phosphoinositide 3-Kinase Cascade Facilitates {micro}-Opioid Desensitization in Sensory Neurons by Altering G-Protein-Effector Interactions J. Neurosci., November 12, 2003; 23(32): 10292 - 10301. [Abstract] [Full Text] [PDF]

				D. E. Kourennyi and S. Barnes Depolarization-Induced Calcium Channel Facilitation in Rod Photoreceptors Is Independent of G Proteins and Phosphorylation J Neurophysiol, July 1, 2000; 84(1): 133 - 138. [Abstract] [Full Text] [PDF]

				T. J. Kamp, H. Hu, and E. Marban Voltage-dependent facilitation of cardiac L-type Ca channels expressed in HEK-293 cells requires beta -subunit Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H126 - H136. [Abstract] [Full Text] [PDF]

				Z. Qiu, D. D. Sweeney, J. G. Netzeband, and D. L. Gruol Chronic Interleukin-6 Alters NMDA Receptor-Mediated Membrane Responses and Enhances Neurotoxicity in Developing CNS Neurons J. Neurosci., December 15, 1998; 18(24): 10445 - 10456. [Abstract] [Full Text] [PDF]

				T. Cens, S. Restituito, A. Vallentin, and P. Charnet Promotion and Inhibition of L-type Ca2+ Channel Facilitation by Distinct Domains of the beta  Subunit J. Biol. Chem., July 17, 1998; 273(29): 18308 - 18315. [Abstract] [Full Text] [PDF]

				P. J. Kammermeier and S. W. Jones Facilitation of L-type Calcium Current in Thalamic Neurons J Neurophysiol, January 1, 1998; 79(1): 410 - 417. [Abstract] [Full Text] [PDF]

				G. Svirskis and J. Hounsgaard Depolarization-Induced Facilitation of a Plateau-Generating Current in Ventral Horn Neurons in the Turtle Spinal Cord J Neurophysiol, September 1, 1997; 78(3): 1740 - 1742. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help