 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 16, Number 16, Issue of August 15, 1996 pp. 5196-5204
Copyright ©1996 Society for NeuroscienceSynchronous Activity in Locus Coeruleus Results from Dendritic Interactions in Pericoerulear Regions
Received March 8, 1996; revised May 31, 1996; accepted June 4, 1996.
Masaru Ishimatsu and John T. Williams The Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201
Locus coeruleus (LC) neurons in brain slices from adult rats were studied using intracellular and extracellular recordings to investigate synchronous activity. Spontaneous field potentials were recorded with extracellular electrodes in solutions containing tetraethylammonium chloride (10 mM) and BaCl2 (1 mM). These field potentials were found throughout but not outside the LC cell body region. No field potentials were observed in control solutions. Paired recordings showed that field potentials were synchronous in all areas of the LC. The synchronous activity was resistant to tetrodotoxin (1 µM) and to the neurotransmitter receptor blockers D-2-amino-5-phosphonopentanoic acid, bicuculline, 6-cyano-7-nitroquinoxaline-2,3-dione, idazoxan, and strychnine, suggesting that this activity was not synaptically driven. Field potentials were also synchronous with oscillations in membrane potential recorded with intracellular electrodes. The oscillations in membrane potential were 5-30 mV in amplitude and had a biphasic waveform. Neither the frequency nor the waveform of the oscillations was dependent on the membrane potential. The glycyrrhetinic acid derivative carbenoxolone and intracellular acidification with CO2 disrupted synchronous activity, suggesting a role of electrotonic coupling. When the cell body region of the LC was isolated from the pericoerulear dendritic regions by sectioning the slice rostral and caudal to the cell body region, synchronous activity was reduced or abolished. Dendritic interaction in the pericoerulear region was also indicated by improved voltage control of the opioid-induced potassium current, as indicated by a shift in the reversal potential to the potassium equilibrium potential. The results suggest that electrical interactions between dendrites outside the cell body region can account for synchronous activity within the nucleus.
Key words: LC; gap junctions; electrotonic coupling; slices; field potentials; dendrites
This article has been cited by other articles:
C. Colomer, L. A. Olivos Ore, N. Coutry, M.-N. Mathieu, S. Arthaud, P. Fontanaud, I. Iankova, F. Macari, E. Thouennon, L. Yon, et al.
Functional Remodeling of Gap Junction-Mediated Electrical Communication between Adrenal Chromaffin Cells in Stressed Rats
J. Neurosci., June 25, 2008; 28(26): 6616 - 6626.
[Abstract] [Full Text] [PDF]
D. G. Placantonakis, A. A. Bukovsky, S. A. Aicher, H.-P. Kiem, and J. P. Welsh
Continuous electrical oscillations emerge from a coupled network: a study of the inferior olive using lentiviral knockdown of connexin36.
J. Neurosci., May 10, 2006; 26(19): 5008 - 5016.
[Abstract] [Full Text] [PDF]
E. Leznik and R. Llinas
Role of Gap Junctions in Synchronized Neuronal Oscillations in the Inferior Olive
J Neurophysiol, October 1, 2005; 94(4): 2447 - 2456.
[Abstract] [Full Text] [PDF]
V. C. Dang and J. T. Williams
Morphine-Induced {micro}-Opioid Receptor Desensitization
Mol. Pharmacol., October 1, 2005; 68(4): 1127 - 1132.
[Abstract] [Full Text] [PDF]
A. O. Martin, G. Alonso, and N. C. Guerineau
Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
J. Cell Biol., May 9, 2005; 169(3): 503 - 514.
[Abstract] [Full Text] [PDF]
D. M. Devilbiss and B. D. Waterhouse
The Effects of Tonic Locus Ceruleus Output on Sensory-Evoked Responses of Ventral Posterior Medial Thalamic and Barrel Field Cortical Neurons in the Awake Rat
J. Neurosci., December 1, 2004; 24(48): 10773 - 10785.
[Abstract] [Full Text] [PDF]
R. W. Putnam, J. A. Filosa, and N. A. Ritucci
Cellular mechanisms involved in CO2 and acid signaling in chemosensitive neurons
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1493 - C1526.
[Abstract] [Full Text] [PDF]
E. Masaki, M. Kawamura, and F. Kato
Attenuation of Gap-Junction-Mediated Signaling Facilitated Anesthetic Effect of Sevoflurane in the Central Nervous System of Rats
Anesth. Analg., March 1, 2004; 98(3): 647 - 652.
[Abstract] [Full Text] [PDF]
E. Garcia-Perez, M. Vargas-Caballero, N. Velazquez-Ulloa, A. Minzoni, and F. F. De-Miguel
Synaptic Integration in Electrically Coupled Neurons
Biophys. J., January 1, 2004; 86(1): 646 - 655.
[Abstract] [Full Text] [PDF]
C. P. Bailey, D. Couch, E. Johnson, K. Griffiths, E. Kelly, and G. Henderson
{micro}-Opioid Receptor Desensitization in Mature Rat Neurons: Lack of Interaction between DAMGO and Morphine
J. Neurosci., November 19, 2003; 23(33): 10515 - 10520.
[Abstract] [Full Text] [PDF]
A. O. Martin, M.-N. Mathieu, and N. C. Guerineau
Evidence for Long-Lasting Cholinergic Control of Gap Junctional Communication between Adrenal Chromaffin Cells
J. Neurosci., May 1, 2003; 23(9): 3669 - 3678.
[Abstract] [Full Text] [PDF]
L. Rela and L. Szczupak
Coactivation of Motoneurons Regulated by a Network Combining Electrical and Chemical Synapses
J. Neurosci., January 15, 2003; 23(2): 682 - 692.
[Abstract] [Full Text] [PDF]
I. C. Solomon, K. H. Chon, and M. N. Rodriguez
Blockade of Brain Stem Gap Junctions Increases Phrenic Burst Frequency and Reduces Phrenic Burst Synchronization in Adult Rat
J Neurophysiol, January 1, 2003; 89(1): 135 - 149.
[Abstract] [Full Text] [PDF]
J. B. Dean, D. Ballantyne, D. L. Cardone, J. S. Erlichman, and I. C. Solomon
Role of gap junctions in CO2 chemoreception and respiratory control
Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L665 - L670.
[Abstract] [Full Text] [PDF]
V. A. Alvarez, S. Arttamangkul, V. Dang, A. Salem, J. L. Whistler, M. von Zastrow, D. K. Grandy, and J. T. Williams
{micro}-Opioid Receptors: Ligand-Dependent Activation of Potassium Conductance, Desensitization, and Internalization
J. Neurosci., July 1, 2002; 22(13): 5769 - 5776.
[Abstract] [Full Text] [PDF]
V. A. Alvarez, C. C. Chow, E. J. Van Bockstaele, and J. T. Williams
Frequency-dependent synchrony in locus ceruleus: Role of electrotonic coupling
PNAS, March 19, 2002; 99(6): 4032 - 4036.
[Abstract] [Full Text] [PDF]
M. Ishimatsu, Y. Kidani, A. Tsuda, and T. Akasu
Effects of Methylphenidate on the Membrane Potential and Current in Neurons of the Rat Locus Coeruleus
J Neurophysiol, March 1, 2002; 87(3): 1206 - 1212.
[Abstract] [Full Text] [PDF]
A. O. Martin, M.-N. Mathieu, C. Chevillard, and N. C. Guerineau
Gap Junctions Mediate Electrical Signaling and Ensuing Cytosolic Ca2+ Increases between Chromaffin Cells in Adrenal Slices: A Role in Catecholamine Release
J. Neurosci., August 1, 2001; 21(15): 5397 - 5405.
[Abstract] [Full Text] [PDF]
S. Arttamangkul, V. Alvarez-Maubecin, G. Thomas, J. T. Williams, and D. K. Grandy
Binding and Internalization of Fluorescent Opioid Peptide Conjugates in Living Cells
Mol. Pharmacol., April 13, 2001; 58(6): 1570 - 1580.
[Abstract] [Full Text]
F. F. De-Miguel, M. Vargas-Caballero, and E. Garcia-Perez
Spread of synaptic potentials through electrical synapses in Retzius neurones of the leech
J. Exp. Biol., January 10, 2001; 204(19): 3241 - 3250.
[Abstract] [Full Text] [PDF]
J. T. Williams, M. J. Christie, and O. Manzoni
Cellular and Synaptic Adaptations Mediating Opioid Dependence
Physiol Rev, January 1, 2001; 81(1): 299 - 343.
[Abstract] [Full Text] [PDF]
R. Kohling, M. Vreugdenhil, E. Bracci, and J. G. R. Jefferys
Ictal Epileptiform Activity Is Facilitated by Hippocampal GABAA Receptor-Mediated Oscillations
J. Neurosci., September 15, 2000; 20(18): 6820 - 6829.
[Abstract] [Full Text] [PDF]
V. Alvarez-Maubecin, F. Garcia-Hernandez, J. T. Williams, and E. J. Van Bockstaele
Functional Coupling between Neurons and Glia
J. Neurosci., June 1, 2000; 20(11): 4091 - 4098.
[Abstract] [Full Text] [PDF]
J. J. Hagan, R. A. Leslie, S. Patel, M. L. Evans, T. A. Wattam, S. Holmes, C. D. Benham, S. G. Taylor, C. Routledge, P. Hemmati, et al.
Orexin A activates locus coeruleus cell firing and increases arousal in the rat
PNAS, September 14, 1999; 96(19): 10911 - 10916.
[Abstract] [Full Text] [PDF]
M. Usher, J. D. Cohen, D. Servan-Schreiber, J. Rajkowski, and G. Aston-Jones
The Role of Locus Coeruleus in the Regulation of Cognitive Performance
Science, January 22, 1999; 283(5401): 549 - 554.
[Abstract] [Full Text]
H. A. Swadlow, I. N. Beloozerova, and M. G. Sirota
Sharp, Local Synchrony Among Putative Feed-Forward Inhibitory Interneurons of Rabbit Somatosensory Cortex
J Neurophysiol, February 1, 1998; 79(2): 567 - 582.
[Abstract] [Full Text] [PDF]
D. Huangfu and P. G. Guyenet
alpha 2-Adrenergic autoreceptors in A5 and A6 neurons of neonate rats
Am J Physiol Heart Circ Physiol, November 1, 1997; 273(5): H2290 - H2295.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|