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Previous Article | Next Article
Volume 16, Number 17, Issue of September 1, 1996 pp. 5312-5323
Copyright ©1996 Society for NeuroscienceCalcium-Dependent Paired-Pulse Facilitation of Miniature EPSC Frequency Accompanies Depression of EPSCs at Hippocampal Synapses in Culture
Received April 29, 1996; revised June 3, 1996; accepted June 5, 1996.
Dana D. Cummings1, Karen S. Wilcox2, and Marc A. Dichter1, 2, 3 1 David Mahoney Institute of Neurological Sciences and 2 Departments of Neurology and 3 Pharmacology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
Two forms of evoked neurotransmitter release at excitatory synapses between cultured hippocampal neurons have been described. After an action potential, it has been shown that transmitter initially is released synchronously, and this is followed by a period of ``slow'' asynchronous release. The ``fast'' synchronous component of release at these synapses has been found routinely to demonstrate paired-pulse and tetanic depression, whereas the short-term plasticity of asynchronous release has not been investigated. In the present experiments, we have used the whole-cell patch-clamp technique to record from pairs of neurons in a low-density hippocampal culture preparation to determine both the properties and underlying mechanisms of short-term plasticity of asynchronous release. It was found that an increase in miniature EPSC (mEPSC) frequency accompanied both single and multiple stimuli, and this mEPSC increase was facilitated during paired stimuli, even when the evoked synchronous release was depressed. In addition, both the activity-dependent depression of evoked EPSCs and facilitation of asynchronous mEPSC release were dependent on Ca accumulation in the nerve terminal. However, the Ca-dependent mechanisms underlying these two processes could be distinguished by the differential effects of two membrane-permeant calcium chelators, BAPTA-AM and EGTA-AM. Frequency-dependent depression of evoked EPSCs involves a rapid rise in intraterminal Ca, which likely triggers a process that proceeds in a Ca-independent manner, whereas the asynchronous release may be linked more directly to a sustained increase in intraterminal Ca.
Key words: mEPSC; synaptic plasticity; BAPTA-AM; EGTA-AM; hippocampal culture; paired-pulse facilitation; paired-pulse depression
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