WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
 QUICK SEARCH:   [advanced]


     
-


HOME
  |  
SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (265)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chang, Y.
Right arrow Articles by Weiss, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, Y.
Right arrow Articles by Weiss, D. S.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH

 Previous Article  |  Next Article 

Volume 16, Number 17, Issue of September 1, 1996 pp. 5415-5424
Copyright ©1996 Society for Neuroscience

Stoichiometry of a Recombinant GABAA Receptor

Received May 1, 1996; revised June 14, 1996; accepted June 18, 1996.

Yongchang Chang, Ruoping Wang, Sonal Barot, and David S. Weiss

Neurobiology Research Center, Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294

GABA is the main inhibitory neurotransmitter in the mammalian brain. The postsynaptic GABAA receptor/pore complex is presumed to be a pentamer typically composed of a combination of alpha , beta , and gamma  subunits, although the stoichiometry remains controversial. We probed the stoichiometry of the GABAA receptor by site-directed mutagenesis of a conserved leucine (to serine) in the putative second membrane-spanning domain of the rat alpha 1(alpha L263S), beta 2(alpha L259S), and gamma 2(alpha L274S) subunit isoforms. Coexpression of wild-type and mutant subunits of each class (e.g., alpha  and alpha L263S), along with their wild-type counterparts (e.g., beta  and gamma ), in Xenopus laevis oocytes resulted in mixed populations of receptors with distinct GABA sensitivities. This is consistent with the interpretation that the leucine mutation increased the GABA sensitivity in proportion to the number of incorporated mutant subunits. The apparent number of incorporated subunits for each class (alpha , beta , and gamma ) could then be determined from the number of components comprising the compound GABA dose-response relationships. Using this approach, we conclude that the recombinant alpha 1beta 2gamma 2 GABAA receptor is a pentamer composed of two alpha  subunits, two beta  subunits, and one gamma  subunit.

Key words: GABA; stoichiometry; receptor; ion channel; mutagenesis




This article has been cited by other articles:


Home page
Mol. Pharmacol.Home page
D. Stewart, R. Desai, Q. Cheng, A. Liu, and S. A. Forman
Tryptophan Mutations at Azi-Etomidate Photo-Incorporation Sites on {alpha}1 or {beta}2 Subunits Enhance GABAA Receptor Gating and Reduce Etomidate Modulation
Mol. Pharmacol., December 1, 2008; 74(6): 1687 - 1695.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
J. Zhang, F. Xue, and Y. Chang
Structural Determinants for Antagonist Pharmacology That Distinguish the {rho}1 GABAC Receptor from GABAA Receptors
Mol. Pharmacol., October 1, 2008; 74(4): 941 - 951.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Mizuta, D. Xu, Y. Pan, G. Comas, J. R. Sonett, Y. Zhang, R. A. Panettieri Jr., J. Yang, and C. W. Emala Sr.
GABAA receptors are expressed and facilitate relaxation in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1206 - L1216.
[Abstract] [Full Text] [PDF]


Home page
JGPHome page
A. Keramidas and N. L. Harrison
Agonist-dependent Single Channel Current and Gating in {alpha}4{beta}2{delta} and {alpha}1{beta}2{gamma}2S GABAA Receptors
J. Gen. Physiol., January 28, 2008; 131(2): 163 - 181.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
K. R. Tan, A. Gonthier, R. Baur, M. Ernst, M. Goeldner, and E. Sigel
Proximity-accelerated Chemical Coupling Reaction in the Benzodiazepine-binding Site of {gamma}-Aminobutyric Acid Type A Receptors: SUPERPOSITION OF DIFFERENT ALLOSTERIC MODULATORS
J. Biol. Chem., September 7, 2007; 282(36): 26316 - 26325.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
S. H. Hadley and J. Amin
Rat {alpha}6beta2{delta} GABAA receptors exhibit two distinct and separable agonist affinities
J. Physiol., June 15, 2007; 581(3): 1001 - 1018.
[Abstract] [Full Text] [PDF]


Home page
Pharmacol. Rev.Home page
J. J. Yi and M. D. Ehlers
Emerging Roles for Ubiquitin and Protein Degradation in Neuronal Function
Pharmacol. Rev., March 1, 2007; 59(1): 14 - 39.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
A. Zeller, M. Arras, R. Jurd, and U. Rudolph
Identification of a Molecular Target Mediating the General Anesthetic Actions of Pentobarbital
Mol. Pharmacol., March 1, 2007; 71(3): 852 - 859.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
I. Sarto-Jackson, R. Furtmueller, M. Ernst, S. Huck, and W. Sieghart
Spontaneous Cross-link of Mutated {alpha}1 Subunits during GABAA Receptor Assembly
J. Biol. Chem., February 16, 2007; 282(7): 4354 - 4363.
[Abstract] [Full Text] [PDF]


Home page
DiabetesHome page
S. J. Bailey, M. A. Ravier, and G. A. Rutter
Glucose-Dependent Regulation of {gamma}-Aminobutyric Acid (GABAA) Receptor Expression in Mouse Pancreatic Islet {alpha}-Cells
Diabetes, February 1, 2007; 56(2): 320 - 327.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
M. R. McCartney, T. Z. Deeb, T. N. Henderson, and T. G. Hales
Tonically Active GABAA Receptors in Hippocampal Pyramidal Neurons Exhibit Constitutive GABA-Independent Gating
Mol. Pharmacol., February 1, 2007; 71(2): 539 - 548.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
A. H. Lagrange, E. J. Botzolakis, and R. L. Macdonald
Enhanced macroscopic desensitization shapes the response of {alpha}4 subtype-containing GABAA receptors to synaptic and extrasynaptic GABA
J. Physiol., February 1, 2007; 578(3): 655 - 676.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
A. Sedelnikova, B. E. Erkkila, H. Harris, S. O. Zakharkin, and D. S. Weiss
Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor
J. Physiol., December 1, 2006; 577(2): 569 - 577.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
Y. Chen, K. Reilly, and Y. Chang
Evolutionarily Conserved Allosteric Network in the Cys Loop Family of Ligand-gated Ion Channels Revealed by Statistical Covariance Analyses
J. Biol. Chem., June 30, 2006; 281(26): 18184 - 18192.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. Serantes, F. Arnalich, M. Figueroa, M. Salinas, E. Andres-Mateos, R. Codoceo, J. Renart, C. Matute, C. Cavada, A. Cuadrado, et al.
Interleukin-1beta Enhances GABAA Receptor Cell-surface Expression by a Phosphatidylinositol 3-Kinase/Akt Pathway: RELEVANCE TO SEPSIS-ASSOCIATED ENCEPHALOPATHY
J. Biol. Chem., May 26, 2006; 281(21): 14632 - 14643.
[Abstract] [Full Text] [PDF]


Home page
J. Med. Genet.Home page
J B Vincent, S. Horike, S Choufani, A D Paterson, W Roberts, P Szatmari, R Weksberg, B Fernandez, and S W Scherer
An inversion inv(4)(p12-p15.3) in autistic siblings implicates the 4p GABA receptor gene cluster
J. Med. Genet., May 1, 2006; 43(5): 429 - 434.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
R. E. Petroski, J. E. Pomeroy, R. Das, H. Bowman, W. Yang, A. P. Chen, and A. C. Foster
Indiplon Is a High-Affinity Positive Allosteric Modulator with Selectivity for {alpha}1 Subunit-Containing GABAA Receptors
J. Pharmacol. Exp. Ther., April 1, 2006; 317(1): 369 - 377.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. M. McCann, J. Bracamontes, J. H. Steinbach, and J. R. Sanes
The cholinergic antagonist {alpha}-bungarotoxin also binds and blocks a subset of GABA receptors
PNAS, March 28, 2006; 103(13): 5149 - 5154.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
M. M. White
Pretty Subunits All in a Row: Using Concatenated Subunit Constructs to Force the Expression of Receptors with Defined Subunit Stoichiometry and Spatial Arrangement
Mol. Pharmacol., February 1, 2006; 69(2): 407 - 410.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
A. J. Boileau, R. A. Pearce, and C. Czajkowski
Tandem Subunits Effectively Constrain GABAA Receptor Stoichiometry and Recapitulate Receptor Kinetics But Are Insensitive to GABAA Receptor-Associated Protein
J. Neurosci., December 7, 2005; 25(49): 11219 - 11230.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
P. V. Plazas, E. Katz, M. E. Gomez-Casati, C. Bouzat, and A. B. Elgoyhen
Stoichiometry of the {alpha}9{alpha}10 Nicotinic Cholinergic Receptor
J. Neurosci., November 23, 2005; 25(47): 10905 - 10912.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. K. Bera and M. H. Akabas
Spontaneous Thermal Motion of the GABAA Receptor M2 Channel-lining Segments
J. Biol. Chem., October 21, 2005; 280(42): 35506 - 35512.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
R. Baur, U. Simmen, M. Senn, U. Sequin, and E. Sigel
Novel Plant Substances Acting as {beta} Subunit Isoform-Selective Positive Allosteric Modulators of GABAA Receptors
Mol. Pharmacol., September 1, 2005; 68(3): 787 - 792.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
D. M. Jones-Davis, L. Song, M. J. Gallagher, and R. L. Macdonald
Structural Determinants of Benzodiazepine Allosteric Regulation of GABAA Receptor Currents
J. Neurosci., August 31, 2005; 25(35): 8056 - 8065.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
R. Baur and E. Sigel
Benzodiazepines Affect Channel Opening of GABAA Receptors Induced by Either Agonist Binding Site
Mol. Pharmacol., April 1, 2005; 67(4): 1005 - 1008.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. Sedelnikova, C. D. Smith, S. O. Zakharkin, D. Davis, D. S. Weiss, and Y. Chang
Mapping the {rho}1 GABAC Receptor Agonist Binding Pocket: CONSTRUCTING A COMPLETE MODEL
J. Biol. Chem., January 14, 2005; 280(2): 1535 - 1542.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Jung, M. H. Akabas, and R. A. Harris
Functional and Structural Analysis of the GABAA Receptor {alpha}1 Subunit during Channel Gating and Alcohol Modulation
J. Biol. Chem., January 7, 2005; 280(1): 308 - 316.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
J. G. Newell, R. A. McDevitt, and C. Czajkowski
Mutation of Glutamate 155 of the GABAA Receptor {beta}2 Subunit Produces a Spontaneously Open Channel: A Trigger for Channel Activation
J. Neurosci., December 15, 2004; 24(50): 11226 - 11235.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
F. Sancar and C. Czajkowski
A GABAA Receptor Mutation Linked to Human Epilepsy ({gamma}2R43Q) Impairs Cell Surface Expression of {alpha}{beta}{gamma} Receptors
J. Biol. Chem., November 5, 2004; 279(45): 47034 - 47039.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
J. Kang and R. L. Macdonald
The GABAA Receptor {gamma}2 Subunit R43Q Mutation Linked to Childhood Absence Epilepsy and Febrile Seizures Causes Retention of {alpha}1{beta}2{gamma}2S Receptors in the Endoplasmic Reticulum
J. Neurosci., October 6, 2004; 24(40): 8672 - 8677.
[Abstract] [Full Text] [PDF]


Home page
JGPHome page
P. J. Groot-Kormelink, S. D. Broadbent, J. P. Boorman, and L. G. Sivilotti
Incomplete Incorporation of Tandem Subunits in Recombinant Neuronal Nicotinic Receptors
J. Gen. Physiol., June 1, 2004; 123(6): 697 - 708.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
B. C. Drafts and J. L. Fisher
Structural Determinants of the Pharmacological Properties of the GABAA Receptor {alpha}6 Subunit
J. Pharmacol. Exp. Ther., June 1, 2004; 309(3): 1108 - 1115.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
F. Minier and E. Sigel
Positioning of the {alpha}-subunit isoforms confers a functional signature to {gamma}-aminobutyric acid type A receptors
PNAS, May 18, 2004; 101(20): 7769 - 7774.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. Rusch, H. Zhong, and S. A. Forman
Gating Allosterism at a Single Class of Etomidate Sites on {alpha}1{beta}2{gamma}2L GABAA Receptors Accounts for Both Direct Activation and Agonist Modulation
J. Biol. Chem., May 14, 2004; 279(20): 20982 - 20992.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
G. Akk, J. Bracamontes, and J. H. Steinbach
Activation of GABAA receptors containing the {alpha}4 subunit by GABA and pentobarbital
J. Physiol., April 15, 2004; 556(2): 387 - 399.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
E. N. Goren, D. C. Reeves, and M. H. Akabas
Loose Protein Packing around the Extracellular Half of the GABAA Receptor {beta}1 Subunit M2 Channel-lining Segment
J. Biol. Chem., March 19, 2004; 279(12): 11198 - 11205.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. Berezhnoy, Y. Nyfeler, A. Gonthier, H. Schwob, M. Goeldner, and E. Sigel
On the Benzodiazepine Binding Pocket in GABAA Receptors
J. Biol. Chem., January 30, 2004; 279(5): 3160 - 3168.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
S. W. Baumann, R. Baur, and E. Sigel
Individual Properties of the Two Functional Agonist Sites in GABAA Receptors
J. Neurosci., December 3, 2003; 23(35): 11158 - 11166.
[Abstract] [Full Text] [PDF]


Home page
NeuroscientistHome page
J. E. Coyle and D. B. Nikolov
GABARAP: Lessons for Synaptogenesis
Neuroscientist, June 1, 2003; 9(3): 205 - 216.
[Abstract] [PDF]


Home page
J. Biol. Chem.Home page
K. Bollan, D. King, L. A. Robertson, K. Brown, P. M. Taylor, S. J. Moss, and C. N. Connolly
GABAA Receptor Composition Is Determined by Distinct Assembly Signals within alpha and beta Subunits
J. Biol. Chem., February 7, 2003; 278(7): 4747 - 4755.
[Abstract] [Full Text] [PDF]


Home page
Anesth. Analg.Home page
D. E. Raines, R. J. Claycomb, and S. A. Forman
Modulation of GABAA Receptor Function by Nonhalogenated Alkane Anesthetics: The Effects on Agonist Enhancement, Direct Activation, and Inhibition
Anesth. Analg., January 1, 2003; 96(1): 112 - 118.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
G. W. Sawyer, D. C. Chiara, R. W. Olsen, and J. B. Cohen
Identification of the Bovine gamma -Aminobutyric Acid Type A Receptor alpha Subunit Residues Photolabeled by the Imidazobenzodiazepine [3H]Ro15-4513
J. Biol. Chem., December 13, 2002; 277(51): 50036 - 50045.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
P. A. Goldstein, F. P. Elsen, S.-W. Ying, C. Ferguson, G. E. Homanics, and N. L. Harrison
Prolongation of Hippocampal Miniature Inhibitory Postsynaptic Currents in Mice Lacking the GABAA Receptor alpha 1 Subunit
J Neurophysiol, December 1, 2002; 88(6): 3208 - 3217.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. W. Baumann, R. Baur, and E. Sigel
Forced Subunit Assembly in alpha 1beta 2gamma 2 GABAA Receptors. INSIGHT INTO THE ABSOLUTE ARRANGEMENT
J. Biol. Chem., November 22, 2002; 277(48): 46020 - 46025.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
E. Palma, V. Esposito, A. M. Mileo, G. Di Gennaro, P. Quarato, F. Giangaspero, C. Scoppetta, P. Onorati, F. Trettel, R. Miledi, et al.
Expression of human epileptic temporal lobe neurotransmitter receptors in Xenopus oocytes: An innovative approach to study epilepsy
PNAS, November 12, 2002; 99(23): 15078 - 15083.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
M. Scheller and S. A. Forman
Coupled and Uncoupled Gating and Desensitization Effects by Pore Domain Mutations in GABAA Receptors
J. Neurosci., October 1, 2002; 22(19): 8411 - 8421.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
I. Sarto, T. Klausberger, N. Ehya, B. Mayer, K. Fuchs, and W. Sieghart
A Novel Site on gamma 3 Subunits Important for Assembly of GABAA Receptors
J. Biol. Chem., August 16, 2002; 277(34): 30656 - 30664.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
M. T. Bianchi, L. Song, H. Zhang, and R. L. Macdonald
Two Different Mechanisms of Disinhibition Produced by GABAA Receptor Mutations Linked to Epilepsy in Humans
J. Neurosci., July 1, 2002; 22(13): 5321 - 5327.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. G. Newell and S. M. J. Dunn
Functional Consequences of the Loss of High Affinity Agonist Binding to gamma -Aminobutyric Acid Type A Receptors. IMPLICATIONS FOR RECEPTOR DESENSITIZATION
J. Biol. Chem., June 7, 2002; 277(24): 21423 - 21430.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. H. Holden and C. Czajkowski
Different Residues in the GABAA Receptor alpha 1T60-alpha 1K70 Region Mediate GABA and SR-95531 Actions
J. Biol. Chem., May 17, 2002; 277(21): 18785 - 18792.
[Abstract] [Full Text] [PDF]


Home page
Physiol. Rev.Home page
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik
Molecular Structure and Physiological Function of Chloride Channels
Physiol Rev, April 1, 2002; 82(2): 503 - 568.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
S.-A. Thompson, P. B. Wingrove, L. Connelly, P. J. Whiting, and K. A. Wafford
Tracazolate Reveals a Novel Type of Allosteric Interaction with Recombinant gamma -Aminobutyric AcidA Receptors
Mol. Pharmacol., April 1, 2002; 61(4): 861 - 869.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
S. B. Christie, C. P. Miralles, and A. L. De Blas
GABAergic Innervation Organizes Synaptic and Extrasynaptic GABAA Receptor Clustering in Cultured Hippocampal Neurons
J. Neurosci., February 1, 2002; 22(3): 684 - 697.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
P. A Davies, E. F Kirkness, and T. G Hales
Evidence for the formation of functionally distinct {alpha}{beta}{gamma}{varepsilon} GABAA receptors
J. Physiol., November 15, 2001; 537(1): 101 - 113.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. E. Adkins, G. V. Pillai, J. Kerby, T. P. Bonnert, C. Haldon, R. M. McKernan, J. E. Gonzalez, K. Oades, P. J. Whiting, and P. B. Simpson
alpha 4beta 3delta GABAA Receptors Characterized by Fluorescence Resonance Energy Transfer-derived Measurements of Membrane Potential
J. Biol. Chem., October 12, 2001; 276(42): 38934 - 38939.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
G. Hapfelmeier, R. Haseneder, E. Kochs, M. Beyerle, and W. Zieglgansberger
Coadministered Nitrous Oxide Enhances the Effect of Isoflurane on GABAergic Transmission by an Increase in Open-Channel Block
J. Pharmacol. Exp. Ther., July 1, 2001; 298(1): 201 - 208.
[Abstract] [Full Text]


Home page
Mol. Pharmacol.Home page
A. J. Smith, L. Alder, J. Silk, C. Adkins, A. E. Fletcher, T. Scales, J. Kerby, G. Marshall, K. A. Wafford, R. M. McKernan, et al.
Effect of alpha Subunit on Allosteric Modulation of Ion Channel Function in Stably Expressed Human Recombinant gamma -Aminobutyric AcidA Receptors Determined Using 36Cl Ion Flux
Mol. Pharmacol., April 16, 2001; 59(5): 1108 - 1118.
[Abstract] [Full Text]


Home page
Mol. Pharmacol.Home page
M. I. Strakhova, S. C. Harvey, C. M. Cook, J. M. Cook, and P. Skolnick
A Single Amino Acid Residue on the alpha 5 Subunit (Ile215) Is Essential for Ligand Selectivity at alpha 5beta 3gamma 2gamma -Aminobutyric AcidA Receptors
Mol. Pharmacol., April 13, 2001; 58(6): 1434 - 1440.
[Abstract] [Full Text]


Home page
J. Neurosci.Home page
M. T. Bianchi, K. F. Haas, and R. L. Macdonald
Structural Determinants of Fast Desensitization and Desensitization-Deactivation Coupling in GABAA Receptors
J. Neurosci., February 15, 2001; 21(4): 1127 - 1136.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
J. P B Boorman, P. J GrootKormelink, and L. G Sivilotti
Stoichiometry of human recombinant neuronal nicotinic receptors containing the {beta}3 subunit expressed in Xenopus oocytes
J. Physiol., December 15, 2000; 529(3): 565 - 577.
[Abstract] [Full Text] [PDF]


Home page
Anesth. Analg.Home page
G. Hapfelmeier, W. Zieglgansberger, R. Haseneder, H. Schneck, and E. Kochs
Nitrous Oxide and Xenon Increase the Efficacy of GABA at Recombinant Mammalian GABAA Receptors
Anesth. Analg., December 1, 2000; 91(6): 1542 - 1549.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
A. S. Cohen, D. D. Lin, and D. A. Coulter
Protracted Postnatal Development of Inhibitory Synaptic Transmission in Rat Hippocampal Area CA1 Neurons
J Neurophysiol, November 1, 2000; 84(5): 2465 - 2476.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
D. B. Williams and M. H. Akabas
Benzodiazepines Induce a Conformational Change in the Region of the gamma -Aminobutyric Acid Type A Receptor alpha 1-Subunit M3 Membrane-Spanning Segment
Mol. Pharmacol., November 1, 2000; 58(5): 1129 - 1136.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
J. G. Newell, M. Davies, A. N. Bateson, and S. M. J. Dunn
Tyrosine 62 of the gamma -Aminobutyric Acid Type A Receptor beta 2 Subunit Is an Important Determinant of High Affinity Agonist Binding
J. Biol. Chem., May 5, 2000; 275(19): 14198 - 14204.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
J. E. Dalziel, G. B. Cox, P. W. Gage, and B. Birnir
Mutating the Highly Conserved Second Membrane-Spanning Region 9' Leucine Residue in the alpha 1 or beta 1 Subunit Produces Subunit-Specific Changes in the Function of Human alpha 1beta 1 gamma -Aminobutyric AcidA Receptors
Mol. Pharmacol., May 1, 2000; 57(5): 875 - 882.
[Abstract] [Full Text]


Home page
Anesth. Analg.Home page
S. Neumahr, G. Hapfelmeier, M. Scheller, H. Schneck, C. Franke, and E. Kochs
Dual Action of Isoflurane on the {gamma}-aminobutyric Acid (GABA)-Mediated Currents Through Recombinant {alpha}1{beta}2{gamma}2L-GABAA-Receptor Channels
Anesth. Analg., May 1, 2000; 90(5): 1184 - 1190.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. Klausberger, K. Fuchs, B. Mayer, N. Ehya, and W. Sieghart
GABAA Receptor Assembly. IDENTIFICATION AND STRUCTURE OF gamma 2 SEQUENCES FORMING THE INTERSUBUNIT CONTACTS WITH alpha 1 AND beta 3 SUBUNITS
J. Biol. Chem., March 17, 2000; 275(12): 8921 - 8928.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
B. X. Carlson, A. C. Engblom, U. Kristiansen, A. Schousboe, and R. W. Olsen
A Single Glycine Residue at the Entrance to the First Membrane-Spanning Domain of the gamma -Aminobutyric Acid Type A Receptor beta 2 Subunit Affects Allosteric Sensitivity to GABA and Anesthetics
Mol. Pharmacol., March 1, 2000; 57(3): 474 - 484.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
S. Srinivasan, C. J. Nichols, G. M. Lawless, R. W. Olsen, and A. J. Tobin
Two Invariant Tryptophans on the alpha 1 Subunit Define Domains Necessary for GABAA Receptor Assembly
J. Biol. Chem., September 17, 1999; 274(38): 26633 - 26638.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
T. R. Neelands and R. L. Macdonald
Incorporation of the pi Subunit into Functional gamma -Aminobutyric AcidA Receptors
Mol. Pharmacol., September 1, 1999; 56(3): 598 - 610.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. USAHome page
T. P. Bonnert, R. M. McKernan, S. Farrar, B. le Bourdelles, R. P. Heavens, D. W. Smith, L. Hewson, M. R. Rigby, D. J. S. Sirinathsinghji, N. Brown, et al.
theta , a novel gamma -aminobutyric acid type A receptor subunit
PNAS, August 17, 1999; 96(17): 9891 - 9896.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
T. R. Neelands, J. Zhang, and R. L. Macdonald
GABAA Receptors Expressed in Undifferentiated Human Teratocarcinoma NT2 Cells Differ from Those Expressed by Differentiated NT2-N Cells
J. Neurosci., August 15, 1999; 19(16): 7057 - 7065.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
C. Sur, S. J. Farrar, J. Kerby, P. J. Whiting, J. R. Atack, and R. M. McKernan
Preferential Coassembly of alpha 4 and delta  Subunits of the gamma -Aminobutyric AcidA Receptor in Rat Thalamus
Mol. Pharmacol., July 1, 1999; 56(1): 110 - 115.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
S. J. Farrar, P. J. Whiting, T. P. Bonnert, and R. M. McKernan
Stoichiometry of a Ligand-gated Ion Channel Determined by Fluorescence Energy Transfer
J. Biol. Chem., April 9, 1999; 274(15): 10100 - 10104.
[Abstract] [Full Text] [PDF]


Home page
Anesth. Analg.Home page
S. Ueno, J. R. Trudell, E. I Eger II, and R. A. Harris
Actions of Fluorinated Alkanols on GABAA Receptors: Relevance to Theories of Narcosis
Anesth. Analg., April 1, 1999; 88(4): 877 - 877.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
A. M L McClellan and R. E Twyman
Receptor system response kinetics reveal functional subtypes of native murine and recombinant human GABAA receptors
J. Physiol., March 15, 1999; 515(3): 711 - 727.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
P. Poisbeau, M. C. Cheney, M. D. Browning, and I. Mody
Modulation of Synaptic GABAA Receptor Function by PKA and PKC in Adult Hippocampal Neurons
J. Neurosci., January 15, 1999; 19(2): 674 - 683.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
T. R. Neelands, J. L. Fisher, M. Bianchi, and R. L. Macdonald
Spontaneous and gamma -Aminobutyric Acid (GABA)-Activated GABAA Receptor Channels Formed by epsilon  Subunit-Containing Isoforms
Mol. Pharmacol., January 1, 1999; 55(1): 168 - 178.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
J. X. Connor, A. J. Boileau, and C. Czajkowski
A GABAA Receptor alpha 1 Subunit Tagged with Green Fluorescent Protein Requires a beta  Subunit for Functional Surface Expression
J. Biol. Chem., October 30, 1998; 273(44): 28906 - 28911.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
T. Defazio and J. J. Hablitz
Zinc and Zolpidem Modulate mIPSCs in Rat Neocortical Pyramidal Neurons
J Neurophysiol, October 1, 1998; 80(4): 1670 - 1677.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
R. M. McKernan, S. Farrar, I. Collins, F. Emms, A. Asuni, K. Quirk, and H. Broughton
Photoaffinity Labeling of the Benzodiazepine Binding Site of alpha 1beta 3gamma 2 gamma -Aminobutyric AcidA Receptors with Flunitrazepam Identifies a Subset of Ligands that Interact Directly with His102 of the alpha  Subunit and Predicts Orientation of These within the Benzodiazepine Pharmacophore
Mol. Pharmacol., July 1, 1998; 54(1): 33 - 43.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
P. J. Groot-Kormelink, W. H. M. L. Luyten, D. Colquhoun, and L. G. Sivilotti
A Reporter Mutation Approach Shows Incorporation of the "Orphan" Subunit beta 3 into a Functional Nicotinic Receptor
J. Biol. Chem., June 19, 1998; 273(25): 15317 - 15320.
[Abstract] [Full Text] [PDF]


Home page
Pharmacol. Rev.Home page
E. A. Barnard, P. Skolnick, R. W. Olsen, H. Mohler, W. Sieghart, G. Biggio, C. Braestrup, A. N. Bateson, and S. Z. Langer
International Union of Pharmacology. XV. Subtypes of gamma -Aminobutyric AcidA Receptors: Classification on the Basis of Subunit Structure and Receptor Function
Pharmacol. Rev., June 1, 1998; 50(2): 291 - 314.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
J. Horenstein and M. H. Akabas
Location of a High Affinity Zn2+ Binding Site in the Channel of alpha 1beta 1 gamma -Aminobutyric AcidA Receptors
Mol. Pharmacol., May 1, 1998; 53(5): 870 - 877.
[Abstract] [Full Text]


Home page
J. Neurosci.Home page
M. Jechlinger, R. Pelz, V. Tretter, T. Klausberger, and W. Sieghart
Subunit Composition and Quantitative Importance of Hetero-oligomeric Receptors: GABAA Receptors Containing alpha 6 Subunits
J. Neurosci., April 1, 1998; 18(7): 2449 - 2457.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
M. D. Krasowski, S. E. Finn, Q. Ye, and N. L. Harrison
Trichloroethanol Modulation of Recombinant GABAA, Glycine and GABA rho 1 Receptors
J. Pharmacol. Exp. Ther., March 1, 1998; 284(3): 934 - 942.
[Abstract] [Full Text]


Home page
Mol. Pharmacol.Home page
Y. Chang and D. S. Weiss
Substitutions of the Highly Conserved M2 Leucine Create Spontaneously Opening rho 1 gamma -Aminobutyric Acid Receptors
Mol. Pharmacol., March 1, 1998; 53(3): 511 - 523.
[Abstract] [Full Text]


Home page
Mol. Pharmacol.Home page
M. D. Krasowski, V. V. Koltchine, C. E. Rick, Q. Ye, S. E. Finn, and N. L. Harrison
Propofol and Other Intravenous Anesthetics Have Sites of Action on the gamma -Aminobutyric Acid Type A Receptor Distinct from That for Isoflurane
Mol. Pharmacol., March 1, 1998; 53(3): 530 - 538.
[Abstract] [Full Text]


Home page
Mol. Pharmacol.Home page
B. Ebert, S. A. Thompson, K. Saounatsou, R. McKernan, P. Krogsgaard-Larsen, and K. A. Wafford
Differences in Agonist/Antagonist Binding Affinity and Receptor Transduction Using Recombinant Human gamma -Aminobutyric Acid Type A Receptors
Mol. Pharmacol., December 1, 1997; 52(6): 1150 - 1156.
[Abstract] [Full Text]


Home page
J. Pharmacol. Exp. Ther.Home page
P. Skolnick, R. J. Hu, C. M. Cook, S. D. Hurt, J. D. Trometer, R. Liu, Q. Huang, and J. M. Cook
[3H]RY 80: A High-Affinity, Selective Ligand for gamma -Aminobutyric AcidA Receptors Containing Alpha-5 Subunits,
J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 488 - 493.
[Abstract] [Full Text]


Home page
Mol. Pharmacol.Home page
P. B. Wingrove, S. A. Thompson, K. A. Wafford, and P. J. Whiting
Key Amino Acids in the gamma  Subunit of the gamma -Aminobutyric AcidA Receptor that Determine Ligand Binding and Modulation at the Benzodiazepine Site
Mol. Pharmacol., November 1, 1997; 52(5): 874 - 881.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. USAHome page
E. Palma, L. Maggi, F. Eusebi, and R. Miledi
Neuronal nicotinic threonine-for-leucine 247 alpha 7 mutant receptors show different gating kinetics when activated by acetylcholine or by the noncompetitive agonist 5-hydroxytryptamine
PNAS, September 2, 1997; 94(18): 9915 - 9919.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
G. H. Gorrie, Y. Vallis, A. Stephenson, J. Whitfield, B. Browning, T. G. Smart, and S. J. Moss
Assembly of GABAA Receptors Composed of alpha 1 and beta 2 Subunits in Both Cultured Neurons and Fibroblasts
J. Neurosci., September 1, 1997; 17(17): 6587 - 6596.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Li and A. L. De Blas
Coexistence of Two beta  Subunit Isoforms in the Same gamma -Aminobutyric Acid Type A Receptor
J. Biol. Chem., June 27, 1997; 272(26): 16564 - 16569.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
V. Tretter, N. Ehya, K. Fuchs, and W. Sieghart
Stoichiometry and Assembly of a Recombinant GABAA Receptor Subtype
J. Neurosci., April 15, 1997; 17(8): 2728 - 2737.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. M. O'Shea and N. L. Harrison
Arg-274 and Leu-277 of the gamma -Aminobutyric Acid Type A Receptor alpha 2 Subunit Define Agonist Efficacy and Potency
J. Biol. Chem., July 21, 2000; 275(30): 22764 - 22768.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. Buhr, C. Wagner, K. Fuchs, W. Sieghart, and E. Sigel
Two Novel Residues in M2 of the gamma -Aminobutyric Acid Type A Receptor Affecting Gating by GABA and Picrotoxin Affinity
J. Biol. Chem., March 9, 2001; 276(11): 7775 - 7781.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. Klausberger, N. Ehya, K. Fuchs, T. Fuchs, V. Ebert, I. Sarto, and W. Sieghart
Detection and Binding Properties of GABAA Receptor Assembly Intermediates
J. Biol. Chem., May 4, 2001; 276(19): 16024 - 16032.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. W. Baumann, R. Baur, and E. Sigel
Subunit Arrangement of gamma -Aminobutyric Acid Type A Receptors
J. Biol. Chem., September 21, 2001; 276(39): 36275 - 36280.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. J. Boileau, J. G. Newell, and C. Czajkowski
GABAA Receptor beta 2 Tyr97 and Leu99 Line the GABA-binding Site. INSIGHTS INTO MECHANISMS OF AGONIST AND ANTAGONIST ACTIONS
J. Biol. Chem., January 18, 2002; 277(4): 2931 - 2937.
[Abstract] [Full Text] [PDF]



-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help

-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-