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Volume 16, Number 17,
Issue of September 1, 1996
pp. 5443-5456
Copyright ©1996 Society for Neuroscience
Traffic of Dynamin within Individual Drosophila
Synaptic Boutons Relative to Compartment-Specific Markers
Received April 30, 1996; revised June 11, 1996; accepted June 12, 1996.
Patricia S. Estes1,
Jack Roos2,
Alexander van der Bliek3,
Regis B. Kelly2,
K. S. Krishnan4, and
Mani Ramaswami1
1 Department of Molecular and Cellular Biology and
Arizona Research Labs, Division of Neurobiology, University of Arizona,
Tucson, Arizona 85721, 2 Department of Biochemistry and
Biophysics and Hormone Research Institute, University of California at
San Francisco, San Francisco, California 94143-0534, 3 Department of Biological Chemistry, University of
California at Los Angeles, Los Angeles, California 90024, and
4 Molecular Biology Unit, Tata Institute for Fundamental
Research, Colaba, Bombay 400005, India
Presynaptic terminals contain several specialized compartments,
which have been described by electron microscopy. We show in an
identified Drosophila neuromuscular synapse that several
of these compartments synaptic vesicle clusters, presynaptic plasma
membrane, presynaptic cytosol, and axonal cytoskeletonlabeled by
specific reagents may be resolved from one another by laser scanning
confocal microscopy. Using a panel of compartment-specific markers and
Drosophila shibirets1 mutants to trap an
intermediate stage in synaptic vesicle recycling, we have examined the
localization and redistribution of dynamin within single synaptic
varicosities at the larval neuromuscular junction. Our results suggest
that dynamin is not a freely diffusible molecule in resting nerve
terminals; rather, it appears localized to synaptic sites by
association with yet uncharacterized presynaptic components. In
shits1 nerve terminals depleted of synaptic
vesicles, dynamin is quantitatively redistributed to the plasma
membrane. It is not, however, distributed uniformly over presynaptic
plasmalemma; instead, fluorescence images show ``hot spots'' of
dynamin on the plasma membrane of vesicle-depleted nerve terminals. We
suggest that these dynamin-rich domains may mark the active zones for
synaptic vesicle endocytosis first described at the frog neuromuscular
junction.
Key words:
neurogenetics;
endocytosis;
membrane traffic;
ultrastructure;
temperature-sensitive paralysis;
synaptic vesicle
recycling;
neurotransmitter release
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