QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (56) Citing Articles via Google Scholar Google Scholar Articles by Warchol, M. E. Articles by Corwin, J. T. Search for Related Content PubMed PubMed Citation Articles by Warchol, M. E. Articles by Corwin, J. T.

 Previous Article  |  Next Article 

Volume 16, Number 17, Issue of September 1, 1996 pp. 5466-5477
Copyright ©1996 Society for Neuroscience

Regenerative Proliferation in Organ Cultures of the Avian Cochlea: Identification of the Initial Progenitors and Determination of the Latency of the Proliferative Response

Received Jan. 18, 1996; revised May 31, 1996; accepted June 5, 1996.

Mark E. Warchol¹ and Jeffrey T. Corwin^{1, 2}

¹ Departments of Otolaryngology-Head and Neck Surgery and ² Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908

Sensory hair cells in the cochleae of birds are regenerated after the death of preexisting hair cells caused by acoustic overstimulation or administration of ototoxic drugs. Regeneration involves renewed proliferation of cells in an epithelium that is otherwise mitotically quiescent. To determine the identity of the first cells that proliferate in response to the death of hair cells and to measure the latency of this proliferative response, we have studied hair-cell regeneration in organ culture. Cochleae from hatchling chicks were placed in culture, and hair cells were killed individually by a laser microbeam. The culture medium was then replaced with a medium that contained a labeled DNA precursor. The treated cochleae were incubated in the labeling media for different time periods before being fixed and processed for the visualization of proliferating cells. The first cells to initiate DNA replication in response to the death of hair cells were supporting cells within the cochlear sensory epithelium. All of the labeled supporting cells were located within 200 µm of the hair-cell lesions. These cells first entered S-phase ~16 hr after the death of hair cells. The results indicate that supporting cells are the precursors of regenerated hair cells and suggest that regenerative proliferation of supporting cells is triggered by signals that act locally within the damaged epithelium.

Key words: hair cell; hearing; ear; regeneration; cochlea; auditory system; precursor

This article has been cited by other articles:

				R. D. Hawkins and M. Lovett The developmental genetics of auditory hair cells Hum. Mol. Genet., October 1, 2004; 13(suppl_2): R289 - R296. [Abstract] [Full Text] [PDF]

				R. D. Hawkins, S. Bashiardes, C. A. Helms, L. Hu, N. L. Saccone, M. E. Warchol, and M. Lovett Gene expression differences in quiescent versus regenerating hair cells of avian sensory epithelia: implications for human hearing and balance disorders Hum. Mol. Genet., June 1, 2003; 12(11): 1261 - 1272. [Abstract] [Full Text] [PDF]

				M. E. Warchol Cell Density and N-Cadherin Interactions Regulate Cell Proliferation in the Sensory Epithelia of the Inner Ear J. Neurosci., April 1, 2002; 22(7): 2607 - 2616. [Abstract] [Full Text] [PDF]

				J. I. Matsui, J. M. Ogilvie, and M. E. Warchol Inhibition of Caspases Prevents Ototoxic and Ongoing Hair Cell Death J. Neurosci., February 15, 2002; 22(4): 1218 - 1227. [Abstract] [Full Text] [PDF]

				M. Montcouquiol and J. T. Corwin Brief Treatments with Forskolin Enhance S-Phase Entry in Balance Epithelia from the Ears of Rats J. Neurosci., February 1, 2001; 21(3): 974 - 982. [Abstract] [Full Text] [PDF]

				J. S. Stone and E. W Rubel Cellular studies of auditory hair cell regeneration in birds PNAS, October 24, 2000; 97(22): 11714 - 11721. [Abstract] [Full Text] [PDF]

				R. A. Baird, M. D. Burton, D. S. Fashena, and R. A. Naeger Hair cell recovery in mitotically blocked cultures of the bullfrog saccule PNAS, October 24, 2000; 97(22): 11722 - 11729. [Abstract] [Full Text] [PDF]

				J. L. Zheng, G. Keller, and W.-Q. Gao Immunocytochemical and Morphological Evidence for Intracellular Self-Repair as an Important Contributor to Mammalian Hair Cell Recovery J. Neurosci., March 15, 1999; 19(6): 2161 - 2170. [Abstract] [Full Text] [PDF]

				D. M. Fekete, S. Muthukumar, and D. Karagogeos Hair Cells and Supporting Cells Share a Common Progenitor in the Avian Inner Ear J. Neurosci., October 1, 1998; 18(19): 7811 - 7821. [Abstract] [Full Text] [PDF]

				J. L. Zheng and W.-Q. Gao Analysis of Rat Vestibular Hair Cell Development and Regeneration Using Calretinin as an Early Marker J. Neurosci., November 1, 1997; 17(21): 8270 - 8282. [Abstract] [Full Text] [PDF]

				J. L. Cyr, A. M. Bell, and A. J. Hudspeth Identification with a recombinant antibody of an inner-ear cytokeratin, a marker for hair-cell differentiation PNAS, April 25, 2000; 97(9): 4908 - 4913. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help