WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience AutoMate Scientific
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (112)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Avery, R. B.
Right arrow Articles by Johnston, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Avery, R. B.
Right arrow Articles by Johnston, D.

Next Article 

Volume 16, Number 18, Issue of September 15, 1996 pp. 5567-5582
Copyright ©1996 Society for Neuroscience

Multiple Channel Types Contribute to the Low-Voltage-Activated Calcium Current in Hippocampal CA3 Pyramidal Neurons

Received April 18, 1996; revised June 17, 1996; accepted June 18, 1996.

Robert B. Avery and Daniel Johnston

Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030

Hippocampal neurons exhibit low-voltage-activated (LVA) and high-voltage-activated (HVA) calcium currents. We characterized the LVA current by recording whole-cell Ca2+ currents from acutely isolated rat hippocampal CA3 pyramidal neurons in 2 m Ca2+.

Long depolarizing steps to -50 mV revealed two components to the LVA current: transient and sustained. The transient phase had a fast decay time constant of 59 msec. The sustained phase persisted throughout the depolarization, even for steps lasting several seconds. The transient current was inhibited by the classic T-type channel antagonists Ni2+ and amiloride. The anticonvulsant phenytoin preferentially blocked the sustained phase, but ethosuximide had no effect. Steady-state inactivation of the transient component was half-maximal at -80 mV.

Nimodipine, an L-type channel antagonist, partly inhibited the sustained current. BayK-8644, an L-type channel agonist, potentiated the sustained current. Calciseptine, another L-type channel antagonist, inhibited the sustained component. omega -Conotoxin-MVIIC, a nonselective toxin for HVA channels, had no effect on either of the LVA current components. omega -Grammotoxin-SIA, another nonselective toxin, partially inhibited the sustained component. The voltage dependence of activation of the nimodipine-sensitive current could be fit with a single Boltzmann, consistent with a homogenous population of L-type channels in CA3 neurons. Half-maximal activation of the nimodipine-sensitive current occurred at -30 mV, considerably more negative than the remaining HVA current.

These results suggest that in physiologic Ca2+ more than one type of Ca2+ channel contributes to the LVA current in CA3 neurons. The transient current is carried by T-type channels. The sustained current is carried, at least in part, by dihydropyridine-sensitive channels. Thus, the designation ``low-voltage-activated'' should not be limited to T-type channels. These findings challenge the traditional designation of L-type channels as exclusively HVA and reveal a possible role in subthreshold Ca2+ signaling.

Key words: calcium channels; hippocampus; CA3; whole-cell patch; low-voltage-activated; T-type; L-type; nimodipine; ethosuximide; phenytoin




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
S. Liu and M. T. Shipley
Multiple Conductances Cooperatively Regulate Spontaneous Bursting in Mouse Olfactory Bulb External Tufted Cells
J. Neurosci., February 13, 2008; 28(7): 1625 - 1639.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. Inoue and B. W. Strowbridge
Transient Activity Induces a Long-Lasting Increase in the Excitability of Olfactory Bulb Interneurons
J Neurophysiol, January 1, 2008; 99(1): 187 - 199.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. Nikitina, Z.-D. Zhang, A. Kawashima, B. S. Jahromi, V. A. Bouryi, M. Takahashi, A. Xie, and R. L. Macdonald
Voltage-dependent calcium channels of dog basilar artery
J. Physiol., April 15, 2007; 580(2): 523 - 541.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Diana, Y. Otsu, G. Maton, T. Collin, M. Chat, and S. Dieudonne
T-Type and L-Type Ca2+ Conductances Define and Encode the Bimodal Firing Pattern of Vestibulocerebellar Unipolar Brush Cells
J. Neurosci., April 4, 2007; 27(14): 3823 - 3838.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Tai, J. B. Kuzmiski, and B. A. MacVicar
Muscarinic enhancement of R-type calcium currents in hippocampal CA1 pyramidal neurons.
J. Neurosci., June 7, 2006; 26(23): 6249 - 6258.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. D. Helton, W. Xu, and D. Lipscombe
Neuronal L-Type Calcium Channels Open Quickly and Are Inhibited Slowly
J. Neurosci., November 2, 2005; 25(44): 10247 - 10251.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Chen, C. Yue, and Y. Yaari
A transitional period of Ca2+-dependent spike afterdepolarization and bursting in developing rat CA1 pyramidal cells
J. Physiol., August 15, 2005; 567(1): 79 - 93.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. A. Olson, T. Tkatch, S. Hernandez-Lopez, S. Ulrich, E. Ilijic, E. Mugnaini, H. Zhang, I. Bezprozvanny, and D. J. Surmeier
G-Protein-Coupled Receptor Modulation of Striatal CaV1.3 L-Type Ca2+ Channels Is Dependent on a Shank-Binding Domain
J. Neurosci., February 2, 2005; 25(5): 1050 - 1062.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. M. Power and P. Sah
Intracellular calcium store filling by an L-type calcium current in the basolateral amygdala at subthreshold membrane potentials
J. Physiol., January 15, 2005; 562(2): 439 - 453.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
D. Lipscombe, T. D. Helton, and W. Xu
L-Type Calcium Channels: The Low Down
J Neurophysiol, November 1, 2004; 92(5): 2633 - 2641.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. C. Jackson, G. L. Yao, and B. P. Bean
Mechanism of Spontaneous Firing in Dorsomedial Suprachiasmatic Nucleus Neurons
J. Neurosci., September 15, 2004; 24(37): 7985 - 7998.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Maravall, E. A. Stern, and K. Svoboda
Development of Intrinsic Properties and Excitability of Layer 2/3 Pyramidal Neurons During a Critical Period for Sensory Maps in Rat Barrel Cortex
J Neurophysiol, July 1, 2004; 92(1): 144 - 156.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Zhou, S.-A. Kim, E. A. Kirk, A. L. Tippens, H. Sun, F. Haeseleer, and A. Lee
Ca2+-Binding Protein-1 Facilitates and Forms a Postsynaptic Complex with Cav1.2 (L-Type) Ca2+ Channels
J. Neurosci., May 12, 2004; 24(19): 4698 - 4708.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
B. R. Johnson, P. Kloppenburg, and R. M. Harris-Warrick
Dopamine Modulation of Calcium Currents in Pyloric Neurons of the Lobster Stomatogastric Ganglion
J Neurophysiol, August 1, 2003; 90(2): 631 - 643.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. J. Habermann, B. J. O'Brien, H. Wassle, and D. A. Protti
AII Amacrine Cells Express L-Type Calcium Channels at Their Output Synapses
J. Neurosci., July 30, 2003; 23(17): 6904 - 6913.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Frick, J. Magee, H. J. Koester, M. Migliore, and D. Johnston
Normalization of Ca2+ Signals by Small Oblique Dendrites of CA1 Pyramidal Neurons
J. Neurosci., April 15, 2003; 23(8): 3243 - 3250.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. S. Isaacson and H. Vitten
GABAB Receptors Inhibit Dendrodendritic Transmission in the Rat Olfactory Bulb
J. Neurosci., March 15, 2003; 23(6): 2032 - 2039.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. Perez-Reyes
Molecular Physiology of Low-Voltage-Activated T-type Calcium Channels
Physiol Rev, January 1, 2003; 83(1): 117 - 161.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Sukhareva, S. V. Smith, D. Maric, and J. L. Barker
Functional Properties of Ryanodine Receptors in Hippocampal Neurons Change During Early Differentiation in Culture
J Neurophysiol, September 1, 2002; 88(3): 1077 - 1087.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
X.-F. Zhang, D. C. Cooper, and F. J. White
Repeated Cocaine Treatment Decreases Whole-Cell Calcium Current in Rat Nucleus Accumbens Neurons
J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 1119 - 1125.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Su, D. Sochivko, A. Becker, J. Chen, Y. Jiang, Y. Yaari, and H. Beck
Upregulation of a T-Type Ca2+ Channel Causes a Long-Lasting Modification of Neuronal Firing Mode after Status Epilepticus
J. Neurosci., May 1, 2002; 22(9): 3645 - 3655.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y. Isomura, Y. Fujiwara-Tsukamoto, M. Imanishi, A. Nambu, and M. Takada
Distance-Dependent Ni2+-Sensitivity of Synaptic Plasticity in Apical Dendrites of Hippocampal CA1 Pyramidal Cells
J Neurophysiol, February 1, 2002; 87(2): 1169 - 1174.
[Abstract] [Full Text] [PDF]

Home page
 Canadian J. AnesthesiaHome page
M. Yamakage and A. Namiki
Calcium channels - basic aspects of their structure, function and gene encoding; anesthetic action on the channels - a review: [Revue : notions de base sur la structure, la fonction et l'encodage genetique des canaux calciques et action des anesthesiques sur ces canaux]
Can J Anesth, February 1, 2002; 49(2): 151 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. C. Gomora, A. N. Daud, M. Weiergraber, and E. Perez-Reyes
Block of Cloned Human T-Type Calcium Channels by Succinimide Antiepileptic Drugs
Mol. Pharmacol., November 1, 2001; 60(5): 1121 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. Xu and D. Lipscombe
Neuronal CaV1.3{alpha}1 L-Type Channels Activate at Relatively Hyperpolarized Membrane Potentials and Are Incompletely Inhibited by Dihydropyridines
J. Neurosci., August 15, 2001; 21(16): 5944 - 5951.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y.-P. Fan, E. M. Horn, and T. G. Waldrop
Biophysical Characterization of Rat Caudal Hypothalamic Neurons: Calcium Channel Contribution to Excitability
J Neurophysiol, December 1, 2000; 84(6): 2896 - 2903.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Shah and D. G. Haylett
Ca2+ Channels Involved in the Generation of the Slow Afterhyperpolarization in Cultured Rat Hippocampal Pyramidal Neurons
J Neurophysiol, May 1, 2000; 83(5): 2554 - 2561.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. G. Mermelstein, H. Bito, K. Deisseroth, and R. W. Tsien
Critical Dependence of cAMP Response Element-Binding Protein Phosphorylation on L-Type Calcium Channels Supports a Selective Response to EPSPs in Preference to Action Potentials
J. Neurosci., January 1, 2000; 20(1): 266 - 273.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. E. Larkum, K. M. M. Kaiser, and B. Sakmann
Calcium electrogenesis in distal apical dendrites of layer 5 pyramidal cells at a critical frequency of back-propagating action potentials
PNAS, December 7, 1999; 96(25): 14600 - 14604.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. C. Magee and M. Carruth
Dendritic Voltage-Gated Ion Channels Regulate the Action Potential Firing Mode of Hippocampal CA1 Pyramidal Neurons
J Neurophysiol, October 1, 1999; 82(4): 1895 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. Morisset and F. Nagy
Ionic Basis for Plateau Potentials in Deep Dorsal Horn Neurons of the Rat Spinal Cord
J. Neurosci., September 1, 1999; 19(17): 7309 - 7316.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. Bianchi, S. R. Young, and R. K. S. Wong
Group I mGluR Activation Causes Voltage-Dependent and -Independent Ca2+ Rises in Hippocampal Pyramidal Cells
J Neurophysiol, June 1, 1999; 81(6): 2903 - 2913.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
H. M. DAVILA
Molecular and Functional Diversity of Voltage-Gated Calcium Channels
Ann. N.Y. Acad. Sci., April 30, 1999; 868(1): 102 - 117.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
E. PEREZ-REYES, J.-H. LEE, and L. L. CRIBBS
Molecular Characterization of Two Members of the T-Type Calcium Channel Family
Ann. N.Y. Acad. Sci., April 30, 1999; 868(1): 131 - 143.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. N. Coogan, D. M. O'Leary, and J. J. O'Connor
P42/44 MAP Kinase Inhibitor PD98059 Attenuates Multiple Forms of Synaptic Plasticity in Rat Dentate Gyrus In Vitro
J Neurophysiol, January 1, 1999; 81(1): 103 - 110.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
T. S. McDowell, J. J. Pancrazio, P. Q. Barrett, and C. Lynch III
Volatile Anesthetic Sensitivity of T-Type Calcium Currents in Various Cell Types
Anesth. Analg., January 1, 1999; 88(1): 168 - 173.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
N. N. Urban, D. A. Henze, and G. Barrionuevo
Amplification of Perforant-Path EPSPs in CA3 Pyramidal Cells by LVA Calcium and Sodium Channels
J Neurophysiol, September 1, 1998; 80(3): 1558 - 1561.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. H. Lee and C. J. Heckman
Bistability in Spinal Motoneurons In Vivo: Systematic Variations in Persistent Inward Currents
J Neurophysiol, August 1, 1998; 80(2): 583 - 593.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Kapur, M. F. Yeckel, R. Gray, and D. Johnston
L-Type Calcium Channels Are Required for One Form of Hippocampal Mossy Fiber LTP
J Neurophysiol, April 1, 1998; 79(4): 2181 - 2190.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. M. Todorovic and C. J. Lingle
Pharmacological Properties of T-Type Ca2+ Current in Adult Rat Sensory Neurons: Effects of Anticonvulsant and Anesthetic Agents
J Neurophysiol, January 1, 1998; 79(1): 240 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Kollmar, L. G. Montgomery, J. Fak, L. J. Henry, and A. J. Hudspeth
Predominance of the alpha 1D subunit in L-type voltage-gated Ca2+ channels of hair cells in the chicken's cochlea
PNAS, December 23, 1997; 94(26): 14883 - 14888.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. S. Piedras-Renteria, C.-C. Chen, and P. M. Best
Antisense oligonucleotides against rat brain alpha 1E DNA and its atrial homologue decrease T-type calcium current in atrial myocytes
PNAS, December 23, 1997; 94(26): 14936 - 14941.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y. Wang, M. J. Rowan, and R. Anwyl
LTP Induction Dependent on Activation of Ni2+-Sensitive Voltage-Gated Calcium Channels, but not NMDA Receptors, in the Rat Dentate Gyrus In Vitro
J Neurophysiol, November 1, 1997; 78(5): 2574 - 2581.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Margeta-Mitrovic, J. J. Grigg, K. Koyano, Y. Nakajima, and S. Nakajima
Neurotensin and Substance P Inhibit Low- and High-Voltage-Activated Ca2+ Channels in Cultured Newborn Rat Nucleus Basalis Neurons
J Neurophysiol, September 1, 1997; 78(3): 1341 - 1352.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. Svirskis and J. Hounsgaard
Depolarization-Induced Facilitation of a Plateau-Generating Current in Ventral Horn Neurons in the Turtle Spinal Cord
J Neurophysiol, September 1, 1997; 78(3): 1740 - 1742.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. T. Kavalali, K. S. Hwang, and M. R. Plummer
cAMP-Dependent Enhancement of Dihydropyridine-Sensitive Calcium Channel Availability in Hippocampal Neurons
J. Neurosci., July 15, 1997; 17(14): 5334 - 5348.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Hernandez-Lopez, J. Bargas, D. J. Surmeier, A. Reyes, and E. Galarraga
D1 Receptor Activation Enhances Evoked Discharge in Neostriatal Medium Spiny Neurons by Modulating an L-Type Ca2+ Conductance
J. Neurosci., May 1, 1997; 17(9): 3334 - 3342.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. Gillessen and C. Alzheimer
Amplification of EPSPs by Low Ni2+- and Amiloride-Sensitive Ca2+ Channels in Apical Dendrites of Rat CA1 Pyramidal Neurons
J Neurophysiol, March 1, 1997; 77(3): 1639 - 1643.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
B. R. Christie, L. K. Schexnayder, and D. Johnston
Contribution of Voltage-Gated Ca2+ Channels to Homosynaptic Long-Term Depression in the CA1 Region In Vitro
J Neurophysiol, March 1, 1997; 77(3): 1651 - 1655.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. B. Avery and D. Johnston
Ca2+ Channel Antagonist U-92032 Inhibits Both T-Type Ca2+ Channels and Na+ Channels in Hippocampal CA1 Pyramidal Neurons
J Neurophysiol, February 1, 1997; 77(2): 1023 - 1028.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Koschak, D. Reimer, I. Huber, M. Grabner, H. Glossmann, J. Engel, and J. Striessnig
alpha 1D (Cav1.3) Subunits Can Form L-type Ca2+ Channels Activating at Negative Voltages
J. Biol. Chem., June 15, 2001; 276(25): 22100 - 22106.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-