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Previous Article | Next Article
Volume 16, Number 18, Issue of September 15, 1996 pp. 5583-5592
Copyright ©1996 Society for NeuroscienceTau Binds to the Distal Axon Early in Development of Polarity in a Microtubule- and Microfilament-Dependent Manner
Received March 12, 1996; revised May 28, 1996; accepted June 19, 1996.
Martina Kempf1, Albrecht Clement1, Andreas Faissner1, Gloria Lee2, and Roland Brandt1 1 Institute of Neurobiology, University of Heidelberg, 69120 Heidelberg, Germany, and 2 Center for Neurological Diseases, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts 02115
Microtubule-associated protein tau is localized to the axon in situ and has been implicated in the development of neuronal polarity. Here we report that tau is extracted differentially in cultured hippocampal neurons yielding an axon-specific localization under conditions that keep the integrity of the plasma membrane. The amount of bound tau increases toward the distal axon and is highest at the transition from the axonal shaft to the growth cone. This distribution is significantly different from the distribution of axonal microtubules that are most concentrated at the proximal axon. Distal binding of tau to one process appears early in development of polarity in culture and correlates with the onset of axon formation (day 2 in culture). Binding to the distal axon requires intact microtubules and microfilaments. Distal tau binding does not stabilize microtubules selectively against drug-induced disassembly, because colchicine-induced microtubule depolymerization is highest distally. We conclude that binding of tau to the distal axon follows a complex mechanism, is an early event in the development of polarity, and reproduces the axon-specific localization of tau in situ.
Key words: tau; microtubule-associated protein; hippocampal neuron; polarity; axon; plasma membrane
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