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Volume 16, Number 18, Issue of September 15, 1996 pp. 5741-5748
Copyright ©1996 Society for Neuroscience

Modulation of the Inhibitory Substrate Properties of Oligodendrocytes by Platelet-Derived Growth Factor

Received May 7, 1996; revised June 21, 1996; accepted June 28, 1996.

Dirk M. Lang¹, Michael G. Hille¹, Martin E. Schwab², and Claudia A. O. Stuermer¹

¹ Faculty of Biology, University of Konstanz, D-78434 Konstanz, Germany, and ² Institute for Brain Research, University of Zürich, CH-8029 Zürich, Switzerland

Although growth cones typically collapse after encountering O1/galactocerebroside (GalC)-positive oligodendrocytes, the majority of growth cones traversed oligodendrocytes, which were raised for 8-10 d in medium containing 10 ng/ml platelet-derived growth factor (PDGF). Oligodendrocytes raised 8-10 d in control medium caused growth cone collapse as they normally do, but failed to elicit this response after being transferred to PDGF-containing medium for an additional 8-10 d. The opposite was observed when PDGF-treated oligodendrocytes were brought to control medium. Growth cones collapsed when contacting these cells. Oligodendrocytes also lost their collapse-inducing activity when raised in medium conditioned by astrocytes, known to produce PDGF. Antibody IN-1 is directed against neurite growth inhibitors (NI), proteins of 35 and 250 kDa on the surface of O1/GalC-positive oligodendrocytes, which are known to elicit growth cone collapse. IN-1 immunoreactivity was markedly reduced in PDGF-treated oligodendrocytes. However, both PDGF-treated and control oligodendrocytes exhibited myelin-associated glycoprotein, proteolipid protein, and myelin basic protein immunoreactivity. This suggests that PDGF-treatment affects NI expression but does not interfere with the expression of advanced myelin marker proteins. Because NI cause growth cone collapse, the loss of collapse-inducing activity by PDGF-treated oligodendrocytes suggests that PDGF regulates, directly or indirectly, the expression of these proteins.

Key words: oligodendrocyte; loss of inhibitory properties; PDGF-treatment; growth cone collapse; IN-1 immunoreactivity; astrocyte-conditioned medium

This article has been cited by other articles:

				C. A.O. Stuermer, D. M. Lang, F. Kirsch, M. Wiechers, S.-O. Deininger, and H. Plattner Glycosylphosphatidyl Inositol-anchored Proteins and fyn Kinase Assemble in Noncaveolar Plasma Membrane Microdomains Defined by Reggie-1 and -2 Mol. Biol. Cell, October 1, 2001; 12(10): 3031 - 3045. [Abstract] [Full Text] [PDF]

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