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Volume 16, Number 18, Issue of September 15, 1996 pp. 5749-5761
Copyright ©1996 Society for Neuroscience

Development of an Identified Spinal Commissural Interneuron Population in an Amniote: Neurons of the Avian Hofmann Nuclei

Received March 21, 1996; revised June 27, 1996; accepted July 2, 1996.

Anne Lill Eide¹ and Joel C. Glover²

¹ Department of Physiology, and ² Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway

The commissural interneurons of the Hofmann nuclei (HN) of the avian spinal cord (The axonal projections of the Hofmann nuclei in the spinal cord of the late stage chicken embryo, Anat Embryol (Berl), A. L. , Vol 193, pp 543-557) provide a unique opportunity to describe the development of an identified spinal commissural axon projection and its terminal collaterals in an amniote vertebrate. Here, we use the lipophilic tracer DiI to label these and other commissural projections anterogradely and retrogradely from the time the HN neurons are born. [³H]thymidine birthdating shows that the final mitoses of HN neurons occur at stages 21-24 [developmental day (d) 4]. By direct comparison, this follows the generation of motoneurons and of large, dorsally located commissural interneurons. The first HN neurons reach the ventrolateral margin of the spinal cord by d6 by a radial migration through the ventral horn. Radial migration occurs after the extension of HN axons across the midline. Thus, HN neurons are determined to be commissural interneurons before attaining their definitive locations. The HN neurons subsequently aggregate into segmentally iterated clusters at the ventrolateral margin of the spinal cord by d8. Also by d8 their longitudinal axons attain mature extent in the ventral funiculus of the contralateral side and begin to sprout collaterals. The collaterals are directed predominantly toward the medial aspect of the ventral horn at all stages, forming by d12 a dense thicket of terminals that thins out over several segments to each side of the HN of origin. The initial direction of collateral outgrowth is largely appropriate for the mature termination pattern of the HN. Terminal arbors, however, are less focused at early developmental stages than at later stages.

Key words: birthdating; spinal interneuron; neuronal migration; axon outgrowth; collateral sprouting; spinal cord; chicken embryo

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