 |
 Previous Article | Next Article 
Volume 16, Number 19,
Issue of October 1, 1996
pp. 6000-6011
Copyright ©1996 Society for Neuroscience
Modulation of High Voltage-Activated Calcium Channels by
Somatostatin in Acutely Isolated Rat Amygdaloid Neurons
Received June 3, 1996; revised July 11, 1996; accepted July 16, 1996.
Félix Viana and
Bertil Hille
Department of Physiology and Biophysics, University of Washington
School of Medicine, Seattle, Washington 98195-7290
We investigated actions of somatostatin (Som) on voltage-gated
calcium channels in acutely isolated rat amygdaloid neurons.
Somatostatin caused a dose-dependent inhibition of the high
voltage-activated (HVA) Ca2+ current, with little or no
effect on the low voltage-activated (LVA) current. Nifedipine (2-10
µM) reduced the peak current by ~15% without reducing
inhibition of current by Som significantly, ruling out L-type channels
as the target of modulation. The modulation appears to involve N- and
P/Q-type calcium channels. After pretreatment with  -conotoxin-GVIA
(-CgTx) or -agatoxin-IVA, the inhibition was reduced but not
abolished, whereas the combined application of both toxins nearly
abolished the modulation. The Som analog BIM-23060 mimicked the effects
of Som, whereas BIM-23058 had no effect, implicating Som type-2
receptors (SSTR-2). The inhibition was voltage-dependent, being minimal
for small depolarizations, and was often accompanied by a slowing of
the activation time course. Strong depolarizing prepulses partially
relieved the inhibition and restored the time course of activation.
Intracellular dialysis with GTP S led to spontaneous inhibition and a
slowing of the current like that with Som and occluded the effects of
the peptide. Dialysis with GDP S also diminished the inhibition. A
short preincubation with 50 µM of the alkylating agent
N-ethylmaleimide (NEM) prevented the action of
somatostatin. These results suggest a role for NEM-sensitive G-proteins
in the Som inhibition. Application of 8-CPT-cAMP and IBMX did not mimic
or prevent the effects of Som.
Key words:
amygdala;
neuropeptide;
G-protein;
NEM;
somatostatin;
calcium channels;
limbic system
This article has been cited by other articles:
|
|
|
|
 
M.-H. Bassant, A. Simon, F. Poindessous-Jazat, Z. Csaba, J. Epelbaum, and P. Dournaud
Medial Septal GABAergic Neurons Express the Somatostatin sst2A Receptor: Functional Consequences on Unit Firing and Hippocampal Theta
J. Neurosci.,
February 23, 2005;
25(8):
2032 - 2041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Wang, N. Matsuoka, S. Mutoh, and S. Kaneko
Modulation of Ca2+Channel Currents by a Novel Antidementia DrugN-(4-Acetyl-1-piperazinyl)-p-fluorobenzamide Monohydrate (FK960) in Rat Hippocampal Neurons
J. Pharmacol. Exp. Ther.,
January 1, 2004;
308(1):
120 - 126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-Y. Lin, M. G. Varma, A. Joubel, S. Madabushi, O. Lichtarge, and D. L. Barber
Conserved Motifs in Somatostatin, D2-dopamine, and alpha 2B-Adrenergic Receptors for Inhibiting the Na-H Exchanger, NHE1
J. Biol. Chem.,
April 18, 2003;
278(17):
15128 - 15135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Oz and L. P. Renaud
Angiotensin AT1-Receptors Depolarize Neonatal Spinal Motoneurons and Other Ventral Horn Neurons Via Two Different Conductances
J Neurophysiol,
November 1, 2002;
88(5):
2857 - 2863.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-Q. Sun, J. R. Huguenard, and D. A. Prince
Somatostatin Inhibits Thalamic Network Oscillations In Vitro: Actions on the GABAergic Neurons of the Reticular Nucleus
J. Neurosci.,
July 1, 2002;
22(13):
5374 - 5386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-B. Gao and A. N van den Pol
Melanin-concentrating hormone depresses L-, N-, and P/Q-type voltage-dependent calcium channels in rat lateral hypothalamic neurons
J. Physiol.,
July 1, 2002;
542(1):
273 - 286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Meis and H.-C. Pape
Control of glutamate and GABA release by nociceptin/orphanin FQ in the rat lateral amygdala
J. Physiol.,
May 1, 2001;
532(3):
701 - 712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-N. Cui, E. Coderre, and L. P. Renaud
GABAB presynaptically modulates suprachiasmatic input to hypothalamic paraventricular magnocellular neurons
Am J Physiol Regulatory Integrative Comp Physiol,
May 1, 2000;
278(5):
R1210 - R1216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Shapiro, J. P. Roche, E. J. Kaftan, H. Cruzblanca, K. Mackie, and B. Hille
Reconstitution of Muscarinic Modulation of the KCNQ2/KCNQ3 K+ Channels That Underlie the Neuronal M Current
J. Neurosci.,
March 1, 2000;
20(5):
1710 - 1721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Acosta and H. S. Lopez
delta Opioid Receptor Modulation of Several Voltage-Dependent Ca2+ Currents in Rat Sensory Neurons
J. Neurosci.,
October 1, 1999;
19(19):
8337 - 8348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. V. Kuzhikandathil and G. S. Oxford
Activation of Human D3 Dopamine Receptor Inhibits P/Q-Type Calcium Channels and Secretory Activity in AtT-20 Cells
J. Neurosci.,
March 1, 1999;
19(5):
1698 - 1707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Overholt and N. R. Prabhakar
Norepinephrine Inhibits a Toxin Resistant Ca2+ Current in Carotid Body Glomus Cells: Evidence for a Direct G Protein Mechanism
J Neurophysiol,
January 1, 1999;
81(1):
225 - 233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Beaumont, M. B. Hepworth, J. S. Luty, E. Kelly, and G. Henderson
Somatostatin Receptor Desensitization in NG108-15 Cells. A CONSEQUENCE OF RECEPTOR SEQUESTRATION
J. Biol. Chem.,
December 11, 1998;
273(50):
33174 - 33183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kolaj and L. P. Renaud
Vasopressin-Induced Currents in Rat Neonatal Spinal Lateral Horn Neurons Are G-Protein Mediated and Involve Two Conductances
J Neurophysiol,
October 1, 1998;
80(4):
1900 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. M. Nakashima, S. M. Todorovic, D. F. Covey, and C. J. Lingle
The Anesthetic Steroid (+)-3alpha -Hydroxy-5alpha -androstane-17beta -carbonitrile Blocks N-, Q-, and R-Type, but Not L- and P-Type, High Voltage-Activated Ca2+ Current in Hippocampal and Dorsal Root Ganglion Neurons of the Rat
Mol. Pharmacol.,
September 1, 1998;
54(3):
559 - 568.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C.-C. Huang, S.-J. Wang, and P.-W. Gean
Selective Enhancement of P-Type Calcium Currents by Isoproterenol in the Rat Amygdala
J. Neurosci.,
March 15, 1998;
18(6):
2276 - 2282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Schweitzer, S. G. Madamba, and G. R. Siggins
Somatostatin Increases a Voltage-Insensitive K+ Conductance in Rat CA1 Hippocampal Neurons
J Neurophysiol,
March 1, 1998;
79(3):
1230 - 1238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-W. Li, P. G. Guyenet, and D. A. Bayliss
Voltage-Dependent Calcium Currents in Bulbospinal Neurons of Neonatal Rat Rostral Ventrolateral Medulla: Modulation by alpha 2-Adrenergic Receptors
J Neurophysiol,
February 1, 1998;
79(2):
583 - 594.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Meis and H.-C. Pape
Properties of a Ca2+-Activated K+ Conductance in Acutely Isolated Pyramidal-Like Neurons From the Rat Basolateral Amygdaloid Complex
J Neurophysiol,
September 1, 1997;
78(3):
1256 - 1262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. White, M. A. Crumling, and S. D. Meriney
Developmental Changes in Calcium Current Pharmacology and Somatostatin Inhibition in Chick Parasympathetic Neurons
J. Neurosci.,
August 15, 1997;
17(16):
6302 - 6313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
