 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 16, 497-505, Copyright © 1996 by Society for Neuroscience
Increased antioxidant enzyme activity in amyloid beta protein-resistant cells
Y Sagara, R Dargusch, FG Klier, D Schubert and C Behl
Salk Institute for Biological Studies, San Diego, California 92186, USA.
Clones of the rat pheochromocytoma cell line PC12 were selected for their
resistance to amyloid beta protein (A beta). These A beta- resistant cells
also survive higher concentrations of exogenously applied peroxides than
the parent cells. A beta triggers intracellular H2O2 accumulation in the
parent PC12 cells but not in the A beta- resistant cells. The absence of
H2O2 accumulation in A beta-resistant cells is not attributable to
differences in A beta binding to the cell surface. However, the mRNA and
protein levels of catalase and glutathione peroxidase, as well as the
corresponding enzyme activities, are highly elevated in A beta-resistant
clones. These activities correlate well with the increased resistance of
cells to A beta or peroxides. Finally, cells transfected with catalase and
glutathione peroxidase are also more resistant to A beta toxicity. These
results indicate that increased antioxidant enzyme activities in A beta-
resistant cells account for at least part of their resistance to A beta and
substantiate further the role of H2O2 in A beta toxicity.
This article has been cited by other articles:
|
|
|
|
 
F. Grodstein, J. H. Kang, R. J. Glynn, N. R. Cook, and J. M. Gaziano
A Randomized Trial of Beta Carotene Supplementation and Cognitive Function in Men: The Physicians' Health Study II
Arch Intern Med,
November 12, 2007;
167(20):
2184 - 2190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Cumming, R. Dargusch, W. H. Fischer, and D. Schubert
Increase in Expression Levels and Resistance to Sulfhydryl Oxidation of Peroxiredoxin Isoforms in Amyloid beta-Resistant Nerve Cells
J. Biol. Chem.,
October 19, 2007;
282(42):
30523 - 30534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Luchsinger, M.-X. Tang, S. Shea, and R. Mayeux
Antioxidant Vitamin Intake and Risk of Alzheimer Disease
Arch Neurol,
February 1, 2003;
60(2):
203 - 208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Opazo, X. Huang, R. A. Cherny, R. D. Moir, A. E. Roher, A. R. White, R. Cappai, C. L. Masters, R. E. Tanzi, N. C. Inestrosa, et al.
Metalloenzyme-like Activity of Alzheimer's Disease beta -Amyloid. Cu-DEPENDENT CATALYTIC CONVERSION OF DOPAMINE, CHOLESTEROL, AND BIOLOGICAL REDUCING AGENTS TO NEUROTOXIC H2O2
J. Biol. Chem.,
October 18, 2002;
277(43):
40302 - 40308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sagara, K. Ishige, C. Tsai, and P. Maher
Tyrphostins Protect Neuronal Cells from Oxidative Stress
J. Biol. Chem.,
September 20, 2002;
277(39):
36204 - 36215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Luchsinger, M.-X. Tang, S. Shea, and R. Mayeux
Caloric Intake and the Risk of Alzheimer Disease
Arch Neurol,
August 1, 2002;
59(8):
1258 - 1263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F-E de Leeuw, J C de Groot, E Achten, M Oudkerk, L M P Ramos, R Heijboer, A Hofman, J Jolles, J van Gijn, and M M B Breteler
Prevalence of cerebral white matter lesions in elderly people: a population based magnetic resonance imaging study. The Rotterdam Scan Study
J. Neurol. Neurosurg. Psychiatry,
January 1, 2001;
70(1):
9 - 14.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Greeve, I. Hermans-Borgmeyer, C. Brellinger, D. Kasper, T. Gomez-Isla, C. Behl, B. Levkau, and R. M. Nitsch
The Human DIMINUTO/DWARF1 Homolog Seladin-1 Confers Resistance to Alzheimer's Disease-Associated Neurodegeneration and Oxidative Stress
J. Neurosci.,
October 1, 2000;
20(19):
7345 - 7352.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Christen
Oxidative stress and Alzheimer disease1
Am. J. Clinical Nutrition,
February 1, 2000;
71(2):
621s - 629s.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Heck, F. Lezoualc'h, S. Engert, and C. Behl
Insulin-like Growth Factor-1-mediated Neuroprotection against Oxidative Stress Is Associated with Activation of Nuclear Factor kappa B
J. Biol. Chem.,
April 2, 1999;
274(14):
9828 - 9835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Gridley, P. S. Green, and J. W. Simpkins
A Novel, Synergistic Interaction Between 17 beta -Estradiol and Glutathione in the Protection of Neurons against beta -Amyloid 25-35-Induced Toxicity In Vitro
Mol. Pharmacol.,
November 1, 1998;
54(5):
874 - 880.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. B. de Haan, C. Bladier, P. Griffiths, M. Kelner, R. D. O'Shea, N. S. Cheung, R. T. Bronson, M. J. Silvestro, S. Wild, S. S. Zheng, et al.
Mice with a Homozygous Null Mutation for the Most Abundant Glutathione Peroxidase, Gpx1, Show Increased Susceptibility to the Oxidative Stress-inducing Agents Paraquat and Hydrogen Peroxide
J. Biol. Chem.,
August 28, 1998;
273(35):
22528 - 22536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. White, H. Zheng, D. Galatis, F. Maher, L. Hesse, G. Multhaup, K. Beyreuther, C. L. Masters, and R. Cappai
Survival of Cultured Neurons from Amyloid Precursor Protein Knock-Out Mice against Alzheimer's Amyloid-beta Toxicity and Oxidative Stress
J. Neurosci.,
August 15, 1998;
18(16):
6207 - 6217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Lezoualc'h, Y. Sagara, F. Holsboer, and C. Behl
High Constitutive NF-kappa B Activity Mediates Resistance to Oxidative Stress in Neuronal Cells
J. Neurosci.,
May 1, 1998;
18(9):
3224 - 3232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Behl, F. Lezoualc'h, T. Trapp, M. Widmann, T. Skutella, and F. Holsboer
Glucocorticoids Enhance Oxidative Stress-Induced Cell Death in Hippocampal Neurons in Vitro
Endocrinology,
January 1, 1997;
138(1):
101 - 106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
