 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 16, 553-562, Copyright © 1996 by Society for Neuroscience
Kinetics of stimulus-coupled secretion in dialyzed bovine chromaffin cells in response to trains of depolarizing pulses
EP Seward and MC Nowycky
Department of Anatomy and Neurobiology, Medical College of Pennsylvania, Philadelphia 19129, USA.
Stimulus-secretion coupling in bovine chromaffin cells was investigated
with whole-cell patch-clamp recordings and capacitance detection techniques
to monitor exocytosis in response to trains of depolarizing pulses. Two
kinetically discrete modes of exocytotic responses were observed. In one
mode, the first depolarization of a train elicited a large increase in
membrane capacitance (Cm; mean approximately 70 fF). This secretory mode
was characterized by small Ca2+ requirements, relative insensitivity to the
pipette Ca2+ chelator concentration, and rapid depletion of the secretory
response. This mode of stimulus- secretion coupling was labile and was seen
only in response to the first and, occasionally, the second stimulus train
of whole-cell recordings. The second type of exocytotic response persisted
for the remainder of the whole-cell recordings and consisted of two
distinct phases. During the earliest pulses of a stimulus train, Ca2+ entry
did not evoke Cm increases. Instead, Cm responses were elicited by later
pulses, despite diminished Ca2+ entry per pulse caused by Ca2+ channel
inactivation. The secretory phase was initiated after a specific
"threshold" amount of Ca2+ had entered the cell, which was determined by
the concentration, but not the binding kinetics, of the Ca2+ chelator in
the pipette. In both the early and the secretory phases, the response of
the cell was proportional to cumulative Ca2+ entry, regardless of current
amplitude, pulse duration, or number of pulses. Threshold-type secretory
kinetics has been described previously in peptide-secreting
neurohypophysial (NHP) nerve terminals (Seward et al., 1995). Secretory
kinetics with minimal Ca2+ requirements has not been observed in that
preparation. Chromaffin cells appear to possess a broader repertoire of
stimulus-secretion coupling modes than NHP terminals.
This article has been cited by other articles:
|
|
|
|
 
D. Zikich, A. Mezer, F. Varoqueaux, A. Sheinin, H. J. Junge, E. Nachliel, R. Melamed, N. Brose, M. Gutman, and U. Ashery
Vesicle Priming and Recruitment by ubMunc13-2 Are Differentially Regulated by Calcium and Calmodulin
J. Neurosci.,
February 20, 2008;
28(8):
1949 - 1960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Rose, S. Efendic, and M. Rupnik
Ca2+-Secretion Coupling Is Impaired in Diabetic Goto Kakizaki rats
J. Gen. Physiol.,
June 1, 2007;
129(6):
493 - 508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. E. Wykes, C. S. Bauer, S. U. Khan, J. L. Weiss, and E. P. Seward
Differential Regulation of Endogenous N- and P/Q-Type Ca2+ Channel Inactivation by Ca2+/Calmodulin Impacts on Their Ability to Support Exocytosis in Chromaffin Cells
J. Neurosci.,
May 9, 2007;
27(19):
5236 - 5248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Bauer, R. J. Woolley, A. G. Teschemacher, and E. P. Seward
Potentiation of Exocytosis by Phospholipase C-Coupled G-Protein-Coupled Receptors Requires the Priming Protein Munc13-1
J. Neurosci.,
January 3, 2007;
27(1):
212 - 219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Garcia, A. M. Garcia-De-Diego, L. Gandia, R. Borges, and J. Garcia-Sancho
Calcium signaling and exocytosis in adrenal chromaffin cells.
Physiol Rev,
October 1, 2006;
86(4):
1093 - 1131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Thiagarajan, T. Tewolde, Y. Li, P. L. Becker, M. M. Rich, and K. L. Engisch
Rab3A negatively regulates activity-dependent modulation of exocytosis in bovine adrenal chromaffin cells
J. Physiol.,
March 1, 2004;
555(2):
439 - 457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.-M. Hur, Y.-S. Park, B. D. Lee, I. H. Jang, H. S. Kim, T.-D. Kim, P.-G. Suh, S. H. Ryu, and K.-T. Kim
Sensitization of Epidermal Growth Factor-induced Signaling by Bradykinin Is Mediated by c-Src: IMPLICATIONS FOR A ROLE OF LIPID MICRODOMAINS
J. Biol. Chem.,
February 13, 2004;
279(7):
5852 - 5860.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Hampson, S.-y. Zhuang, J. L. Weiner, and S. A. Deadwyler
Functional Significance of Cannabinoid-Mediated, Depolarization-Induced Suppression of Inhibition (DSI) in the Hippocampus
J Neurophysiol,
July 1, 2003;
90(1):
55 - 64.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Xu, Y. Xu, G. C. R. Ellis-Davies, G. J. Augustine, and F. W. Tse
Differential Regulation of Exocytosis by alpha - and beta -SNAPs
J. Neurosci.,
January 1, 2002;
22(1):
53 - 61.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Brezina, P. J. Church, and K. R. Weiss
Temporal Pattern Dependence of Neuronal Peptide Transmitter Release: Models and Experiments
J. Neurosci.,
September 15, 2000;
20(18):
6760 - 6772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Teschemacher and E. P. Seward
Bidirectional Modulation of Exocytosis by Angiotensin II Involves Multiple G-Protein-Regulated Transduction Pathways in Chromaffin Cells
J. Neurosci.,
July 1, 2000;
20(13):
4776 - 4785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Powell, A. G. Teschemacher, and E. P. Seward
P2Y Purinoceptors Inhibit Exocytosis in Adrenal Chromaffin Cells via Modulation of Voltage-Operated Calcium Channels
J. Neurosci.,
January 15, 2000;
20(2):
606 - 616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Meir, S. Ginsburg, A. Butkevich, S. G. Kachalsky, I. Kaiserman, R. Ahdut, S. Demirgoren, and R. Rahamimoff
Ion Channels in Presynaptic Nerve Terminals and Control of Transmitter Release
Physiol Rev,
July 1, 1999;
79(3):
1019 - 1088.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Engisch, M. M. Rich, N. Cook, and M. C. Nowycky
Lambert-Eaton Antibodies Inhibit Ca2+ Currents But Paradoxically Increase Exocytosis during Stimulus Trains in Bovine Adrenal Chromaffin Cells
J. Neurosci.,
May 1, 1999;
19(9):
3384 - 3395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Smith
A Persistent Activity-Dependent Facilitation in Chromaffin Cells Is Caused by Ca2+ Activation of Protein Kinase C
J. Neurosci.,
January 15, 1999;
19(2):
589 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. D. Mansvelder and K. S. Kits
The Relation of Exocytosis and Rapid Endocytosis to Calcium Entry Evoked by Short Repetitive Depolarizing Pulses in Rat Melanotropic Cells
J. Neurosci.,
January 1, 1998;
18(1):
81 - 92.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Engisch, N. I. Chernevskaya, and M. C. Nowycky
Short-Term Changes in the Ca2+-Exocytosis Relationship during Repetitive Pulse Protocols in Bovine Adrenal Chromaffin Cells
J. Neurosci.,
December 1, 1997;
17(23):
9010 - 9025.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Smith and E. Neher
Multiple Forms of Endocytosis In Bovine Adrenal Chromaffin Cells
J. Cell Biol.,
November 17, 1997;
139(4):
885 - 894.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Moser and E. Neher
Rapid Exocytosis in Single Chromaffin Cells Recorded from Mouse Adrenal Slices
J. Neurosci.,
April 1, 1997;
17(7):
2314 - 2323.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. von Gersdorff and G. Matthews
Depletion and Replenishment of Vesicle Pools at a Ribbon-Type Synaptic Terminal
J. Neurosci.,
March 15, 1997;
17(6):
1919 - 1927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
