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Journal of Neuroscience, Vol 16, 563-571, Copyright © 1996 by Society for Neuroscience
Recovery phase of the murine rod photoresponse reconstructed from electroretinographic recordings
AL Lyubarsky and EN Pugh Jr
Department of Psychology, University of Pennsylvania, Philadelphia 19104-6196, USA.
The activation and recovery phases of the murine rod photo-response were
determined from corneal electroretinograms (ERGs) obtained in response to
pairs of full-field flashes producing 50-10(5) photoisomerized rhodopsins
(R*) per rod. The a-wave component of the ERG in response to the initial
flash provided a well established measure of the activation phase of the
rod response. The amplitude of the a-wave response to an intense second
flash (45,000 R*) delivered 0.2-5 seconds (s) after the first flash was
used to reconstruct the recovery phase of the response. For 160-3000 R*
rod-1, recovery curves were isomorphic, translating on the time axis such
that each e-fold increase in R* produced an incremental recovery delay of
tau c = 210 +/- 50 ms (mean +/- SD). For initial flashes producing >
3000 R*, recovery curves lost their initial isomorphism and half-times had
intensity dependence exceeding 1 s per e-fold increase in R*. We conclude
that for flashes producing < 3000 R*, the effective lifetime of these R*
is not > 210 ms. Two extant and non-mutually exclusive hypotheses are
discussed that can account for the sharp increase in recovery times from
flashes producing > 3000 R*. They are as follows: (1) approximately
0.03% of R* have a lifetime exceeding 1 s; and (2) the gamma subunit of
phosphodiesterase (PDE gamma) serves as a GTPase- activating factor, and
3000 R* produce sufficient activated G-protein (G*) to exceed the total
quantity of PDE gamma subunits such that excess G* must wait for unoccupied
PDE gamma to inactivate via GTP hydrolysis.
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