 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 16, 631-639, Copyright © 1996 by Society for Neuroscience
Neuronal activity differentially regulates NMDA receptor subunit expression in cerebellar granule cells
ML Vallano, B Lambolez, E Audinat and J Rossier
Department of Pharmacology, State University of New York Health Sciences Center, Syracuse 13210, USA.
Reverse-transcription PCR assays were used to measure levels of NMDA
receptor (NR) subunit mRNAs encoding splice variants of NR1 (NR1a, - exon
5; NR1b, +exon 5) and the major NR2 subunits (NR2A, NR2B, and NR2C) in
dissociated cerebellar granule cell cultures. Cultures chronically exposed
to 25 mM KCl or 100 microM NMDA/15 mM KCl, which promote survival by
stimulating Ca2+ influx through voltage-sensitive Ca2+ channels or NRs,
were compared with 5 mM KCl culture conditions, which results in limited
cell survival attributable to a lower level of NR stimulation by ambient
glutamate. In situ granule-cell maturation is associated with
downregulation of NR2B and increases both of NR2A and NR2C and in the ratio
of NR1b/NR1a mRNAs. In culture, 25 mM KCl or NMDA rapidly induced NR2A and
downregulated NR2B, followed by gradual induction of NR2C. In 5 mM KCl, a
similar, rapid increase in NR2A was observed, but disappearance of NR2B
occurred over a longer time course. By 9-12 d in vitro in 5 mM KCl, the
relative proportions of all three NR2 mRNAs in surviving cells were not
significantly different from cells cultured in 25 mM KCl. NR1a mRNA
predominated at every stage of culture in 25 mM KCl or NMDA, however,
whereas gradual induction of the mature-form NR1b was observed in 5 mM KCl.
Although using high potassium- or NMDA-containing media enhanced granule
cell survival, it did not reproduce the pattern of expression of NR mRNAs
observed in situ, whereas this pattern was observed in granule cells
surviving in 5 mM KCl.
This article has been cited by other articles:
|
|
|
|
 
K. Iijima, H. Abe, M. Okazawa, K. Moriyoshi, and S. Nakanishi
Dual regulation of NR2B and NR2C expression by NMDA receptor activation in mouse cerebellar granule cell cultures
PNAS,
August 19, 2008;
105(33):
12010 - 12015.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lu, Z. Fu, I. Karavanov, R. P. Yasuda, B. B. Wolfe, A. Buonanno, and S. Vicini
NMDA Receptor Subtypes at Autaptic Synapses of Cerebellar Granule Neurons
J Neurophysiol,
November 1, 2006;
96(5):
2282 - 2294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nakanishi and M. Okazawa
Membrane potential-regulated Ca2+ signalling in development and maturation of mammalian cerebellar granule cells
J. Physiol.,
September 1, 2006;
575(2):
389 - 395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Monaco III and M. L. Vallano
Roscovitine Triggers Excitotoxicity in Cultured Granule Neurons by Enhancing Glutamate Release
Mol. Pharmacol.,
November 1, 2005;
68(5):
1331 - 1342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. J. Moody and M. M. Bosma
Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells
Physiol Rev,
July 1, 2005;
85(3):
883 - 941.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-H. Chen, C.-T. Wei, Y.-R. Lin, T.-H. Chien, and M.-H. Chan
Neonatal Toluene Exposure Alters Agonist and Antagonist Sensitivity and NR2B Subunit Expression of NMDA Receptors in Cultured Cerebellar Granule Neurons
Toxicol. Sci.,
May 1, 2005;
85(1):
666 - 674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Fu, S. M Logan, and S. Vicini
Deletion of the NR2A subunit prevents developmental changes of NMDA-mEPSCs in cultured mouse cerebellar granule neurones
J. Physiol.,
March 15, 2005;
563(3):
867 - 881.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Tremper-Wells and M. L. Vallano
Nuclear Calpain Regulates Ca2+-dependent Signaling via Proteolysis of Nuclear Ca2+/Calmodulin-dependent Protein Kinase Type IV in Cultured Neurons
J. Biol. Chem.,
January 21, 2005;
280(3):
2165 - 2175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Choi, C. M. Beaman-Hall, and M. L. Vallano
Granule neurons in cerebellum express distinct splice variants of the inositol trisphosphate receptor that are modulated by calcium
Am J Physiol Cell Physiol,
October 1, 2004;
287(4):
C971 - C980.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Simeone, R. M. Sanchez, and J. M. Rho
Molecular Biology and Ontogeny of Glutamate Receptors in the Mammalian Central Nervous System
J Child Neurol,
May 1, 2004;
19(5):
343 - 360.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. Liu, Z. Zhuang, P. W. Hoffman, and G. Bai
Functional Analysis of the Rat N-Methyl-D-aspartate Receptor 2A Promoter: MULTIPLE TRANSCRIPTION START POINTS, POSITIVE REGULATION BY Sp FACTORS, AND TRANSLATIONAL REGULATION
J. Biol. Chem.,
July 11, 2003;
278(29):
26423 - 26434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Engblom, F. F. Johansen, and U. Kristiansen
Actions and Interactions of Extracellular Potassium and Kainate on Expression of 13 gamma -Aminobutyric Acid Type A Receptor Subunits in Cultured Mouse Cerebellar Granule Neurons
J. Biol. Chem.,
May 2, 2003;
278(19):
16543 - 16550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Colonnese, J. Shi, and M. Constantine-Paton
Chronic NMDA Receptor Blockade From Birth Delays the Maturation of NMDA Currents, but Does Not Affect AMPA/Kainate Currents
J Neurophysiol,
January 1, 2003;
89(1):
57 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Clayton, M. H. Mesches, E. Alvarez, P. C. Bickford, and M. D. Browning
A Hippocampal NR2B Deficit Can Mimic Age-Related Changes in Long-Term Potentiation and Spatial Learning in the Fischer 344 Rat
J. Neurosci.,
May 1, 2002;
22(9):
3628 - 3637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-H. Huh and R. J. Wenthold
Turnover Analysis of Glutamate Receptors Identifies a Rapidly Degraded Pool of the N-Methyl-D-aspartate Receptor Subunit, NR1, in Cultured Cerebellar Granule Cells
J. Biol. Chem.,
January 1, 1999;
274(1):
151 - 157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Qiu, D. D. Sweeney, J. G. Netzeband, and D. L. Gruol
Chronic Interleukin-6 Alters NMDA Receptor-Mediated Membrane Responses and Enhances Neurotoxicity in Developing CNS Neurons
J. Neurosci.,
December 15, 1998;
18(24):
10445 - 10456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Brandoli, A. Sanna, M. A. De Bernardi, P. Follesa, G. Brooker, and I. Mocchetti
Brain-Derived Neurotrophic Factor and Basic Fibroblast Growth Factor Downregulate NMDA Receptor Function in Cerebellar Granule Cells
J. Neurosci.,
October 1, 1998;
18(19):
7953 - 7961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Mellor, D. Merlo, A. Jones, W. Wisden, and A. D. Randall
Mouse Cerebellar Granule Cell Differentiation: Electrical Activity Regulates the GABAA Receptor alpha 6 Subunit Gene
J. Neurosci.,
April 15, 1998;
18(8):
2822 - 2833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-q. J. Liu and L. K. Kaczmarek
The Expression of Two Splice Variants of the Kv3.1 Potassium Channel Gene Is Regulated by Different Signaling Pathways
J. Neurosci.,
April 15, 1998;
18(8):
2881 - 2890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vicini, J. F. Wang, J. H. Li, W. J. Zhu, Y. H. Wang, J. H. Luo, B. B. Wolfe, and D. R. Grayson
Functional and Pharmacological Differences Between Recombinant N-Methyl-D-Aspartate Receptors
J Neurophysiol,
February 1, 1998;
79(2):
555 - 566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D'Angelo, G. De Filippi, P. Rossi, and V. Taglietti
Synaptic Activation of Ca2+ Action Potentials in Immature Rat Cerebellar Granule Cells In Situ
J Neurophysiol,
September 1, 1997;
78(3):
1631 - 1642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Legendre
Pharmacological Evidence for Two Types of Postsynaptic Glycinergic Receptors on the Mauthner Cell of 52-h-Old Zebrafish Larvae
J Neurophysiol,
May 1, 1997;
77(5):
2400 - 2415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Gault and R. E. Siegel
Expression of the GABAA Receptor delta Subunit Is Selectively Modulated by Depolarization in Cultured Rat Cerebellar Granule Neurons
J. Neurosci.,
April 1, 1997;
17(7):
2391 - 2399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Flint, U. S. Maisch, J. H. Weishaupt, A. R. Kriegstein, and H. Monyer
NR2A Subunit Expression Shortens NMDA Receptor Synaptic Currents in Developing Neocortex
J. Neurosci.,
April 1, 1997;
17(7):
2469 - 2476.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kadotani, T. Hirano, M. Masugi, K. Nakamura, K. Nakao, M. Katsuki, and S. Nakanishi
Motor Discoordination Results from Combined Gene Disruption of the NMDA Receptor NR2A and NR2C Subunits, But Not from Single Disruption of the NR2A or NR2C Subunit
J. Neurosci.,
December 15, 1996;
16(24):
7859 - 7867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sasner and A. Buonanno
Distinct N-Methyl--aspartate Receptor 2B Subunit Gene Sequences Confer Neural and Developmental Specific Expression
J. Biol. Chem.,
August 30, 1996;
271(35):
21316 - 21322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Daniels and D. R. Brown
Astrocytes Regulate N-Methyl-D-aspartate Receptor Subunit Composition Increasing Neuronal Sensitivity to Excitotoxicity
J. Biol. Chem.,
June 15, 2001;
276(25):
22446 - 22452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
