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Next Article 
Volume 16, Number 20,
Issue of October 15, 1996
pp. 6331-6341
Copyright ©1996 Society for Neuroscience
Immunohistochemical Distribution and Electron Microscopic
Subcellular Localization of the Proteasome in the Rat CNS
Received June 14, 1996; revised July 18, 1996; accepted July 22, 1996.
Elisa Mengual1,
Paz Arizti2,
José Rodrigo3,
José Manuel Giménez-Amaya1, and
José
G. Castaño2
Departamentos de 1 Morfología and
2 Bioquímica e Instituto de Investigaciones
Biomédicas del CSIC, Facultad de Medicina, Universidad
Autónoma de Madrid, 28029 Madrid, Spain, and
3 Instituto Cajal, CSIC, 28029 Madrid, Spain
The proteasome multicatalytic proteinase (MCP) is a 20S complex
that plays a major role in nonlysosomal pathways of intracellular
protein degradation. A polyclonal antibody against rat liver MCP was
used to investigate the distribution of MCP in the CNS of the rat and
its subcellular localization within the neurons. As expected, MCP
immunoreactivity (MCP-IR) was distributed ubiquitously in the rat CNS
but not homogeneously. The most intensely stained neurons were the
pyramidal cortical neurons of layer 5 and the motor neurons of the
ventral horn in the spinal cord, which show an intense nuclear and
cytoplasmatic MCP-IR and clearly stained processes. Additionally, some
populations of large neurons in the mesencephalon and brainstem also
displayed a moderate MCP-IR in their perikarya. The vast majority of
neurons in the remaining structures did not show a strong cytoplasmatic
MCP-IR, but their nuclei displayed an intense MCP-IR. The subcellular
localization also was studied by immunoelectron microscopy. MCP-IR was
intense in the neuronal nuclei, and significant staining also was found
in the cytoplasm, dendritic, and axonic processes (including some
myelinated axons) and in synaptic boutons, as illustrated in the
cerebellar cortex. The distribution of MCP in the rat CNS and its
subcellular localization are discussed in relation to (1) the
distribution of calpain, the other major nonlysosomal cellular
protease, and (2) the possible role of MCP in the degradation of
regulatory proteins and key transcription factors that are essential in
many neuronal responses.
Key words:
multicatalytic proteinase;
proteasome;
prosome;
immunohistochemistry;
immunoelectron microscopy;
CNS;
rat
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