QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (152) Citing Articles via Google Scholar Google Scholar Articles by Sodickson, D. L. Articles by Bean, B. P. Search for Related Content PubMed PubMed Citation Articles by Sodickson, D. L. Articles by Bean, B. P.

 Previous Article  |  Next Article 

Volume 16, Number 20, Issue of October 15, 1996 pp. 6374-6385
Copyright ©1996 Society for Neuroscience

GABA_B Receptor-Activated Inwardly Rectifying Potassium Current in Dissociated Hippocampal CA3 Neurons

Received May 31, 1996; revised July 26, 1996; accepted July 30, 1996.

Deborah L. Sodickson and Bruce P. Bean

Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201, and Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115

GABA and the GABA_B receptor agonist baclofen activated a potassium conductance in acutely dissociated hippocampal CA3 neurons. Baclofen-activated current required internal GTP, was purely potassium selective, and showed strong inward rectification. As with acetylcholine-activated current in atrial myocytes, external Cs⁺ blocked inward but not outward current in a highly voltage-dependent manner, whereas Ba²⁺ blocked with no voltage dependence. Unlike the cardiac current, however, the baclofen-activated current showed no intrinsic voltage-dependent relaxation. With fast solution exchange, current was activated by baclofen or GABA with a lag of ~50 msec followed by an exponential phase (time constant ~225 msec at saturating agonist concentrations); deactivation was preceded by a lag of ~150 msec and occurred with a time constant of ~1 sec. GABA activated the potassium conductance with a half maximally effective concentration (EC₅₀) of 1.6 µM, much lower than that for activation of GABA_A receptor-activated chloride current in the same cells (EC₅₀ ~25 µM). At low GABA concentrations, activation of the GABA_B current had a Hill coefficient of 1.4-2.1, suggesting cooperativity in the receptor-to-channel pathway. Although the maximal conductance activated by GABA_B receptors is much smaller than that activated by GABA_A receptors, its higher sensitivity to GABA and slower time course make it well suited to respond to low concentrations of extra-synaptic GABA.

Key words: baclofen; G-protein; GIRK; GABA; GABA_A; chloride current; cesium; barium

This article has been cited by other articles:

				Y. Kamikubo, T. Tabata, S. Kakizawa, D. Kawakami, M. Watanabe, A. Ogura, M. Iino, and M. Kano Postsynaptic GABAB receptor signalling enhances LTD in mouse cerebellar Purkinje cells J. Physiol., December 1, 2007; 585(2): 549 - 563. [Abstract] [Full Text] [PDF]

				U. Kim and L.-y. Chung Dual GABAergic Synaptic Response of Fast Excitation and Slow Inhibition in the Medial Habenula of Rat Epithalamus J Neurophysiol, September 1, 2007; 98(3): 1323 - 1332. [Abstract] [Full Text] [PDF]

				A. M. Pooler and R. A. J. McIlhinney Lateral Diffusion of the GABAB Receptor Is Regulated by the GABAB2 C Terminus J. Biol. Chem., August 31, 2007; 282(35): 25349 - 25356. [Abstract] [Full Text] [PDF]

				J.-W. Sohn, D. Lee, H. Cho, W. Lim, H.-S. Shin, S.-H. Lee, and W.-K. Ho Receptor-specific inhibition of GABAB-activated K+ currents by muscarinic and metabotropic glutamate receptors in immature rat hippocampus J. Physiol., April 15, 2007; 580(2): 411 - 422. [Abstract] [Full Text] [PDF]

				T. K. Best, R. J. Siarey, and Z. Galdzicki Ts65Dn, a Mouse Model of Down Syndrome, Exhibits Increased GABAB-Induced Potassium Current J Neurophysiol, January 1, 2007; 97(1): 892 - 900. [Abstract] [Full Text] [PDF]

				A. Kulik, I. Vida, Y. Fukazawa, N. Guetg, Y. Kasugai, C. L. Marker, F. Rigato, B. Bettler, K. Wickman, M. Frotscher, et al. Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells. J. Neurosci., April 19, 2006; 26(16): 4289 - 4297. [Abstract] [Full Text] [PDF]

				S. Chevallier, F. Nagy, and J.-M. Cabelguen Cholinergic control of excitability of spinal motoneurones in the salamander J. Physiol., February 1, 2006; 570(3): 525 - 540. [Abstract] [Full Text] [PDF]

				M. Nobles, A. Benians, and A. Tinker Heterotrimeric G proteins precouple with G protein-coupled receptors in living cells PNAS, December 20, 2005; 102(51): 18706 - 18711. [Abstract] [Full Text] [PDF]

				C. Acuna-Goycolea and A. N. van den Pol Peptide YY3-36 Inhibits Both Anorexigenic Proopiomelanocortin and Orexigenic Neuropeptide Y Neurons: Implications for Hypothalamic Regulation of Energy Homeostasis J. Neurosci., November 9, 2005; 25(45): 10510 - 10519. [Abstract] [Full Text] [PDF]

				R. I. Wilson and G. Laurent Role of GABAergic Inhibition in Shaping Odor-Evoked Spatiotemporal Patterns in the Drosophila Antennal Lobe J. Neurosci., October 5, 2005; 25(40): 9069 - 9079. [Abstract] [Full Text] [PDF]

				C. Acuna-Goycolea, N. Tamamaki, Y. Yanagawa, K. Obata, and A. N. van den Pol Mechanisms of Neuropeptide Y, Peptide YY, and Pancreatic Polypeptide Inhibition of Identified Green Fluorescent Protein-Expressing GABA Neurons in the Hypothalamic Neuroendocrine Arcuate Nucleus J. Neurosci., August 10, 2005; 25(32): 7406 - 7419. [Abstract] [Full Text] [PDF]

				M. T. Ghorbel, K. G. Becker, and J. M. Henley Profile of changes in gene expression in cultured hippocampal neurones evoked by the GABAB receptor agonist baclofen Physiol Genomics, June 16, 2005; 22(1): 93 - 98. [Abstract] [Full Text] [PDF]

				J. R. Pugh and I. M. Raman GABAA Receptor Kinetics in the Cerebellar Nuclei: Evidence for Detection of Transmitter from Distant Release Sites Biophys. J., March 1, 2005; 88(3): 1740 - 1754. [Abstract] [Full Text] [PDF]

				Y. Li and A. N. van den Pol Direct and Indirect Inhibition by Catecholamines of Hypocretin/Orexin Neurons J. Neurosci., January 5, 2005; 25(1): 173 - 183. [Abstract] [Full Text] [PDF]

				O. J. Ariwodola and J. L. Weiner Ethanol Potentiation of GABAergic Synaptic Transmission May Be Self-Limiting: Role of Presynaptic GABAB Receptors J. Neurosci., November 24, 2004; 24(47): 10679 - 10686. [Abstract] [Full Text] [PDF]

				J. T. Porter and D. Nieves Presynaptic GABAB Receptors Modulate Thalamic Excitation of Inhibitory and Excitatory Neurons in the Mouse Barrel Cortex J Neurophysiol, November 1, 2004; 92(5): 2762 - 2770. [Abstract] [Full Text] [PDF]

				L.-Y. Fu, C. Acuna-Goycolea, and A. N. van den Pol Neuropeptide Y Inhibits Hypocretin/Orexin Neurons by Multiple Presynaptic and Postsynaptic Mechanisms: Tonic Depression of the Hypothalamic Arousal System J. Neurosci., October 6, 2004; 24(40): 8741 - 8751. [Abstract] [Full Text] [PDF]

				W. W. Wu, C. S. Chan, and J. F. Disterhoft Slow Afterhyperpolarization Governs the Development of NMDA Receptor-Dependent Afterdepolarization in CA1 Pyramidal Neurons During Synaptic Stimulation J Neurophysiol, October 1, 2004; 92(4): 2346 - 2356. [Abstract] [Full Text] [PDF]

				P. Schweitzer, M. Roberto, S. G. Madamba, and G. R. Siggins {gamma}-Hydroxybutyrate Increases a Potassium Current and Decreases the H-Current in Hippocampal Neurons via GABAB Receptors J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 172 - 179. [Abstract] [Full Text] [PDF]

				C. Acuna-Goycolea and A. van den Pol Glucagon-Like Peptide 1 Excites Hypocretin/Orexin Neurons by Direct and Indirect Mechanisms: Implications for Viscera-Mediated Arousal J. Neurosci., September 15, 2004; 24(37): 8141 - 8152. [Abstract] [Full Text] [PDF]

				S. G. A. Frere and A. Luthi Pacemaker channels in mouse thalamocortical neurones are regulated by distinct pathways of cAMP synthesis J. Physiol., January 1, 2004; 554(1): 111 - 125. [Abstract] [Full Text] [PDF]

				K. Caesar, K. Thomsen, and M. Lauritzen Dissociation of spikes, synaptic activity, and activity-dependent increments in rat cerebellar blood flow by tonic synaptic inhibition PNAS, December 23, 2003; 100(26): 16000 - 16005. [Abstract] [Full Text] [PDF]

				A. Kulik, I. Vida, R. Lujan, C. A. Haas, G. Lopez-Bendito, R. Shigemoto, and M. Frotscher Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus J. Neurosci., December 3, 2003; 23(35): 11026 - 11035. [Abstract] [Full Text] [PDF]

				Q.-Q. Sun, S. C. Baraban, D. A. Prince, and J. R. Huguenard Target-Specific Neuropeptide Y-Ergic Synaptic Inhibition and Its Network Consequences within the Mammalian Thalamus J. Neurosci., October 22, 2003; 23(29): 9639 - 9649. [Abstract] [Full Text] [PDF]

				T. Sickmann and C. Alzheimer Short-Term Desensitization of G-Protein-Activated, Inwardly Rectifying K+ (GIRK) Currents in Pyramidal Neurons of Rat Neocortex J Neurophysiol, October 1, 2003; 90(4): 2494 - 2503. [Abstract] [Full Text] [PDF]

				S. D. Brenowitz and W. G. Regehr Calcium Dependence of Retrograde Inhibition by Endocannabinoids at Synapses onto Purkinje Cells J. Neurosci., July 16, 2003; 23(15): 6373 - 6384. [Abstract] [Full Text] [PDF]

				A. Benians, J. L. Leaney, and A. Tinker Agonist unbinding from receptor dictates the nature of deactivation kinetics of G protein-gated K+ channels PNAS, May 13, 2003; 100(10): 6239 - 6244. [Abstract] [Full Text] [PDF]

				J. S. Isaacson and H. Vitten GABAB Receptors Inhibit Dendrodendritic Transmission in the Rat Olfactory Bulb J. Neurosci., March 15, 2003; 23(6): 2032 - 2039. [Abstract] [Full Text] [PDF]

				A. Benians, J. L. Leaney, G. Milligan, and A. Tinker The Dynamics of Formation and Action of the Ternary Complex Revealed in Living Cells Using a G-protein-gated K+ Channel as a Biosensor J. Biol. Chem., March 14, 2003; 278(12): 10851 - 10858. [Abstract] [Full Text] [PDF]

				Y. A. Blednov, M. Stoffel, H. Alva, and R. A. Harris A pervasive mechanism for analgesia: Activation of GIRK2 channels PNAS, January 7, 2003; 100(1): 277 - 282. [Abstract] [Full Text] [PDF]

				T. A. Simeone, S. D. Donevan, and J. M. Rho Molecular Biology and Ontogeny of {gamma}-Aminobutyric Acid (GABA) Receptors in the Mammalian Central Nervous System J Child Neurol, January 1, 2003; 18(1): 39 - 48. [Abstract] [PDF]

				J. Mitterdorfer and B. P. Bean Potassium Currents during the Action Potential of Hippocampal CA3 Neurons J. Neurosci., December 1, 2002; 22(23): 10106 - 10115. [Abstract] [Full Text] [PDF]

				R. Sadja, N. Alagem, and E. Reuveny Graded contribution of the Gbeta gamma binding domains to GIRK channel activation PNAS, August 6, 2002; 99(16): 10783 - 10788. [Abstract] [Full Text] [PDF]

				M. Havlickova, L. Prezeau, B. Duthey, B. Bettler, J.-P. Pin, and J. Blahos The Intracellular Loops of the GB2 Subunit Are Crucial for G-Protein Coupling of the Heteromeric gamma -Aminobutyrate B Receptor Mol. Pharmacol., August 1, 2002; 62(2): 343 - 350. [Abstract] [Full Text] [PDF]

				Q.-Q. Sun, J. R. Huguenard, and D. A. Prince Somatostatin Inhibits Thalamic Network Oscillations In Vitro: Actions on the GABAergic Neurons of the Reticular Nucleus J. Neurosci., July 1, 2002; 22(13): 5374 - 5386. [Abstract] [Full Text] [PDF]

				V. Lopantsev and P. A. Schwartzkroin GABAA-Dependent Chloride Influx Modulates Reversal Potential of GABAB-Mediated IPSPs in Hippocampal Pyramidal Cells J Neurophysiol, June 1, 2001; 85(6): 2381 - 2387. [Abstract] [Full Text] [PDF]

				M. Catsicas and P. Mobbs GABAB Receptors Regulate Chick Retinal Calcium Waves J. Neurosci., February 1, 2001; 21(3): 897 - 910. [Abstract] [Full Text] [PDF]

				J. T. Williams, M. J. Christie, and O. Manzoni Cellular and Synaptic Adaptations Mediating Opioid Dependence Physiol Rev, January 1, 2001; 81(1): 299 - 343. [Abstract] [Full Text] [PDF]

				L. S. Overstreet, M. V. Jones, and G. L. Westbrook Slow Desensitization Regulates the Availability of Synaptic GABAA Receptors J. Neurosci., November 1, 2000; 20(21): 7914 - 7921. [Abstract] [Full Text] [PDF]

				V. Aroniadou-Anderjaska, F.-M. Zhou, C. A. Priest, M. Ennis, and M. T. Shipley Tonic and Synaptically Evoked Presynaptic Inhibition of Sensory Input to the Rat Olfactory Bulb Via GABAB Heteroreceptors J Neurophysiol, September 1, 2000; 84(3): 1194 - 1203. [Abstract] [Full Text] [PDF]

				P. Lorente, A. Lacampagne, Y. Pouzeratte, S. Richards, B. Malitschek, R. Kuhn, B. Bettler, and G. Vassort gamma -Aminobutyric acid type B receptors are expressed and functional in mammalian cardiomyocytes PNAS, July 18, 2000; 97(15): 8664 - 8669. [Abstract] [Full Text] [PDF]

				H. Blumenfeld and D. A. McCormick Corticothalamic Inputs Control the Pattern of Activity Generated in Thalamocortical Networks J. Neurosci., July 1, 2000; 20(13): 5153 - 5162. [Abstract] [Full Text] [PDF]

				A. Kovoor, C.-K. Chen, W. He, T. G. Wensel, M. I. Simon, and H. A. Lester Co-expression of Gbeta 5 Enhances the Function of Two Ggamma Subunit-like Domain-containing Regulators of G Protein Signaling Proteins J. Biol. Chem., February 4, 2000; 275(5): 3397 - 3402. [Abstract] [Full Text] [PDF]

				G. J. Greif, D. L. Sodickson, B. P. Bean, E. J. Neer, and U. Mende Altered Regulation of Potassium and Calcium Channels by GABAB and Adenosine Receptors in Hippocampal Neurons From Mice Lacking Galpha o J Neurophysiol, February 1, 2000; 83(2): 1010 - 1018. [Abstract] [Full Text] [PDF]

				V. Lopantsev and P. A. Schwartzkroin GABAA-Dependent Chloride Influx Modulates GABAB-Mediated IPSPs in Hippocampal Pyramidal Cells J Neurophysiol, September 1, 1999; 82(3): 1218 - 1223. [Abstract] [Full Text] [PDF]

				D. D. Mott, Q. Li, M. M. Okazaki, D. A. Turner, and D. V. Lewis GABAB-Receptor-Mediated Currents in Interneurons of the Dentate-Hilus Border J Neurophysiol, September 1, 1999; 82(3): 1438 - 1450. [Abstract] [Full Text] [PDF]

				E. B. Stevens, B. S. Shah, R. D. Pinnock, and K. Lee Bombesin Receptors Inhibit G Protein-Coupled Inwardly Rectifying K+ Channels Expressed in Xenopus Oocytes through a Protein Kinase C-Dependent Pathway Mol. Pharmacol., June 1, 1999; 55(6): 1020 - 1027. [Abstract] [Full Text]

				P. Sah and J. D. Clements Photolytic Manipulation of [Ca2+]i Reveals Slow Kinetics of Potassium Channels Underlying the Afterhyperpolarization in Hipppocampal Pyramidal Neurons J. Neurosci., May 15, 1999; 19(10): 3657 - 3664. [Abstract] [Full Text] [PDF]

				Z. Shao and A. Burkhalter Role of GABAB Receptor-Mediated Inhibition in Reciprocal Interareal Pathways of Rat Visual Cortex J Neurophysiol, March 1, 1999; 81(3): 1014 - 1024. [Abstract] [Full Text] [PDF]

				L. Sun and R. J. Miller Multiple Neuropeptide Y Receptors Regulate K+ and Ca2+ Channels in Acutely Isolated Neurons From the Rat Arcuate Nucleus J Neurophysiol, March 1, 1999; 81(3): 1391 - 1403. [Abstract] [Full Text] [PDF]

				K. Kaupmann, V. Schuler, J. Mosbacher, S. Bischoff, H. Bittiger, J. Heid, W. Froestl, S. Leonhard, T. Pfaff, A. Karschin, et al. Human gamma -aminobutyric acid type B receptors are differentially expressed and regulate inwardly rectifying K+ channels PNAS, December 8, 1998; 95(25): 14991 - 14996. [Abstract] [Full Text] [PDF]

				N. J. Berman and L. Maler Inhibition Evoked From Primary Afferents in the Electrosensory Lateral Line Lobe of the Weakly Electric Fish (Apteronotus leptorhynchus) J Neurophysiol, December 1, 1998; 80(6): 3173 - 3196. [Abstract] [Full Text] [PDF]

				N. J. Berman and L. Maler Interaction of GABAB-Mediated Inhibition With Voltage-Gated Currents of Pyramidal Cells: Computational Mechanism of a Sensory Searchlight J Neurophysiol, December 1, 1998; 80(6): 3197 - 3213. [Abstract] [Full Text] [PDF]

				M. Yamada, A. Inanobe, and Y. Kurachi G Protein Regulation of Potassium Ion Channels Pharmacol. Rev., December 1, 1998; 50(4): 723 - 757. [Abstract] [Full Text] [PDF]

				U. Kim and D. A. McCormick The Functional Influence of Burst and Tonic Firing Mode on Synaptic Interactions in the Thalamus J. Neurosci., November 15, 1998; 18(22): 9500 - 9516. [Abstract] [Full Text] [PDF]

				A. Destexhe Spike-and-Wave Oscillations Based on the Properties of GABAB Receptors J. Neurosci., November 1, 1998; 18(21): 9099 - 9111. [Abstract] [Full Text] [PDF]

				D. L. Sodickson and B. P. Bean Neurotransmitter Activation of Inwardly Rectifying Potassium Current in Dissociated Hippocampal CA3 Neurons: Interactions among Multiple Receptors J. Neurosci., October 15, 1998; 18(20): 8153 - 8162. [Abstract] [Full Text] [PDF]

				K. R. Svoboda and C. R. Lupica Opioid Inhibition of Hippocampal Interneurons via Modulation of Potassium and Hyperpolarization-Activated Cation (Ih) Currents J. Neurosci., September 15, 1998; 18(18): 7084 - 7098. [Abstract] [Full Text] [PDF]

				J. S. Isaacson GABAB Receptor-Mediated Modulation of Presynaptic Currents and Excitatory Transmission at a Fast Central Synapse J Neurophysiol, September 1, 1998; 80(3): 1571 - 1576. [Abstract] [Full Text] [PDF]

W. Jarolimek, J. Baurle,